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Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay

Vancomycin-resistant enterococci (VREs) have become one of the most important nosocomial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral fl...

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Autores principales: Oueslati, Saoussen, Gonzalez, Camille, Volland, Hervé, Cattoir, Vincent, Bernabeu, Sandrine, Girlich, Delphine, Dulac, Duncan, Plaisance, Marc, Boutigny, Laure, Dortet, Laurent, Simon, Stéphanie, Naas, Thierry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534553/
https://www.ncbi.nlm.nih.gov/pubmed/34679500
http://dx.doi.org/10.3390/diagnostics11101805
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author Oueslati, Saoussen
Gonzalez, Camille
Volland, Hervé
Cattoir, Vincent
Bernabeu, Sandrine
Girlich, Delphine
Dulac, Duncan
Plaisance, Marc
Boutigny, Laure
Dortet, Laurent
Simon, Stéphanie
Naas, Thierry
author_facet Oueslati, Saoussen
Gonzalez, Camille
Volland, Hervé
Cattoir, Vincent
Bernabeu, Sandrine
Girlich, Delphine
Dulac, Duncan
Plaisance, Marc
Boutigny, Laure
Dortet, Laurent
Simon, Stéphanie
Naas, Thierry
author_sort Oueslati, Saoussen
collection PubMed
description Vancomycin-resistant enterococci (VREs) have become one of the most important nosocomial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VREs) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100% when bacterial cells were grown in the presence of vancomycin used as a VanB inducer. The NG-Test VanB is an efficient, rapid and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs from colonies. Together with the NG-Test VanA, they could replace the already existing tests available for the confirmation of acquired vancomycin resistance in enterococci, especially from selective media or from antibiograms, with 100% sensitivity and specificity. Rapid detection in less than 15 min will result in more efficient management of carriers and infected patients. In addition, these tests may be used for positive blood cultures, given a 3.5 h sub-culturing step on Chocolate agar PolyViteX in the presence of a 5-µg vancomycin disk, which is routinely performed in many clinical microbiology laboratories for every positive blood culture for subsequent MALDI-TOF identification of the growing bacteria.
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spelling pubmed-85345532021-10-23 Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay Oueslati, Saoussen Gonzalez, Camille Volland, Hervé Cattoir, Vincent Bernabeu, Sandrine Girlich, Delphine Dulac, Duncan Plaisance, Marc Boutigny, Laure Dortet, Laurent Simon, Stéphanie Naas, Thierry Diagnostics (Basel) Article Vancomycin-resistant enterococci (VREs) have become one of the most important nosocomial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VREs) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100% when bacterial cells were grown in the presence of vancomycin used as a VanB inducer. The NG-Test VanB is an efficient, rapid and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs from colonies. Together with the NG-Test VanA, they could replace the already existing tests available for the confirmation of acquired vancomycin resistance in enterococci, especially from selective media or from antibiograms, with 100% sensitivity and specificity. Rapid detection in less than 15 min will result in more efficient management of carriers and infected patients. In addition, these tests may be used for positive blood cultures, given a 3.5 h sub-culturing step on Chocolate agar PolyViteX in the presence of a 5-µg vancomycin disk, which is routinely performed in many clinical microbiology laboratories for every positive blood culture for subsequent MALDI-TOF identification of the growing bacteria. MDPI 2021-09-29 /pmc/articles/PMC8534553/ /pubmed/34679500 http://dx.doi.org/10.3390/diagnostics11101805 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oueslati, Saoussen
Gonzalez, Camille
Volland, Hervé
Cattoir, Vincent
Bernabeu, Sandrine
Girlich, Delphine
Dulac, Duncan
Plaisance, Marc
Boutigny, Laure
Dortet, Laurent
Simon, Stéphanie
Naas, Thierry
Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay
title Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay
title_full Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay
title_fullStr Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay
title_full_unstemmed Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay
title_short Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay
title_sort rapid detection of vana/b-producing vancomycin-resistant enterococci using lateral flow immunoassay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534553/
https://www.ncbi.nlm.nih.gov/pubmed/34679500
http://dx.doi.org/10.3390/diagnostics11101805
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