Cargando…
The Use of Saliva as a Biosample in the Light of COVID-19
Saliva is easy to collect and a biofluid that is readily available without the need for special equipment for its collection. The collection process, which is non-invasive and inexpensive, leads to obtaining a biomaterial that can serve as a source of information for molecular diagnostics of disease...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534561/ https://www.ncbi.nlm.nih.gov/pubmed/34679467 http://dx.doi.org/10.3390/diagnostics11101769 |
_version_ | 1784587582509678592 |
---|---|
author | Duś-Ilnicka, Irena Krala, Elżbieta Cholewińska, Paulina Radwan-Oczko, Małgorzata |
author_facet | Duś-Ilnicka, Irena Krala, Elżbieta Cholewińska, Paulina Radwan-Oczko, Małgorzata |
author_sort | Duś-Ilnicka, Irena |
collection | PubMed |
description | Saliva is easy to collect and a biofluid that is readily available without the need for special equipment for its collection. The collection process, which is non-invasive and inexpensive, leads to obtaining a biomaterial that can serve as a source of information for molecular diagnostics of diseases in general medicine, genetics and dentistry. Unfortunately, many of the salivary methodologies are lacking important parameters to provide for not only the safety of the operator, but also the quality and reproducibility of the research. Since the COVID-19 pandemic, salivary diagnostics demonstrate a great potential for research of SARS-CoV 2. In this review, good practice for unstimulated saliva collection and patient preparation was provided, based on the latest literature and available guidelines. Schemes for saliva collection procedures were presented following an extended literature search. Descriptions of salivary probes/cups, techniques of saliva collection, and the use of specific buffering solutions for the stability of collected samples for SARS-CoV-2 detection were also evaluated. |
format | Online Article Text |
id | pubmed-8534561 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85345612021-10-23 The Use of Saliva as a Biosample in the Light of COVID-19 Duś-Ilnicka, Irena Krala, Elżbieta Cholewińska, Paulina Radwan-Oczko, Małgorzata Diagnostics (Basel) Review Saliva is easy to collect and a biofluid that is readily available without the need for special equipment for its collection. The collection process, which is non-invasive and inexpensive, leads to obtaining a biomaterial that can serve as a source of information for molecular diagnostics of diseases in general medicine, genetics and dentistry. Unfortunately, many of the salivary methodologies are lacking important parameters to provide for not only the safety of the operator, but also the quality and reproducibility of the research. Since the COVID-19 pandemic, salivary diagnostics demonstrate a great potential for research of SARS-CoV 2. In this review, good practice for unstimulated saliva collection and patient preparation was provided, based on the latest literature and available guidelines. Schemes for saliva collection procedures were presented following an extended literature search. Descriptions of salivary probes/cups, techniques of saliva collection, and the use of specific buffering solutions for the stability of collected samples for SARS-CoV-2 detection were also evaluated. MDPI 2021-09-26 /pmc/articles/PMC8534561/ /pubmed/34679467 http://dx.doi.org/10.3390/diagnostics11101769 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Duś-Ilnicka, Irena Krala, Elżbieta Cholewińska, Paulina Radwan-Oczko, Małgorzata The Use of Saliva as a Biosample in the Light of COVID-19 |
title | The Use of Saliva as a Biosample in the Light of COVID-19 |
title_full | The Use of Saliva as a Biosample in the Light of COVID-19 |
title_fullStr | The Use of Saliva as a Biosample in the Light of COVID-19 |
title_full_unstemmed | The Use of Saliva as a Biosample in the Light of COVID-19 |
title_short | The Use of Saliva as a Biosample in the Light of COVID-19 |
title_sort | use of saliva as a biosample in the light of covid-19 |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534561/ https://www.ncbi.nlm.nih.gov/pubmed/34679467 http://dx.doi.org/10.3390/diagnostics11101769 |
work_keys_str_mv | AT dusilnickairena theuseofsalivaasabiosampleinthelightofcovid19 AT kralaelzbieta theuseofsalivaasabiosampleinthelightofcovid19 AT cholewinskapaulina theuseofsalivaasabiosampleinthelightofcovid19 AT radwanoczkomałgorzata theuseofsalivaasabiosampleinthelightofcovid19 AT dusilnickairena useofsalivaasabiosampleinthelightofcovid19 AT kralaelzbieta useofsalivaasabiosampleinthelightofcovid19 AT cholewinskapaulina useofsalivaasabiosampleinthelightofcovid19 AT radwanoczkomałgorzata useofsalivaasabiosampleinthelightofcovid19 |