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A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)

Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-S...

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Autores principales: Peng, Yu-Chi, Cheng, Chia-Hsuan, Yatsuda, Hiromi, Liu, Szu-Heng, Liu, Shih-Jen, Kogai, Takashi, Kuo, Chen-Yen, Wang, Robert Y. L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534600/
https://www.ncbi.nlm.nih.gov/pubmed/34679536
http://dx.doi.org/10.3390/diagnostics11101838
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author Peng, Yu-Chi
Cheng, Chia-Hsuan
Yatsuda, Hiromi
Liu, Szu-Heng
Liu, Shih-Jen
Kogai, Takashi
Kuo, Chen-Yen
Wang, Robert Y. L.
author_facet Peng, Yu-Chi
Cheng, Chia-Hsuan
Yatsuda, Hiromi
Liu, Szu-Heng
Liu, Shih-Jen
Kogai, Takashi
Kuo, Chen-Yen
Wang, Robert Y. L.
author_sort Peng, Yu-Chi
collection PubMed
description Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 (p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing.
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spelling pubmed-85346002021-10-23 A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW) Peng, Yu-Chi Cheng, Chia-Hsuan Yatsuda, Hiromi Liu, Szu-Heng Liu, Shih-Jen Kogai, Takashi Kuo, Chen-Yen Wang, Robert Y. L. Diagnostics (Basel) Article Since the Coronavirus disease 2019 (COVID-19) pandemic outbreak, many methods have been used to detect antigens or antibodies to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), including viral culture, nucleic acid test, and immunoassay. The shear-horizontal surface acoustic wave (SH-SAW) biosensor is a novel pathogen detection platform with the advantages of high sensitivity and short detection time. The objective of this study is to develop a SH-SAW biosensor to detect the anti-SARS-CoV-2 nucleocapsid antibody. The rabbit sera collected from rabbits on different days after SARS-CoV-2 N protein injection were evaluated by SH-SAW biosensor and enzyme-linked immunosorbent assay (ELISA). The results showed that the SH-SAW biosensor achieved a high correlation coefficient (R = 0.9997) with different concentrations (34.375–1100 ng/mL) of the “spike-in” anti-N protein antibodies. Compared to ELISA, the SH-SAW biosensor has better sensitivity and can detect anti-N protein IgG signals earlier than ELISA on day 6 (p < 0.05). Overall, in this study, we demonstrated that the SH-SAW biosensor is a promising platform for rapid in vitro diagnostic (IVD) testing, especially for antigen or antibody testing. MDPI 2021-10-05 /pmc/articles/PMC8534600/ /pubmed/34679536 http://dx.doi.org/10.3390/diagnostics11101838 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Peng, Yu-Chi
Cheng, Chia-Hsuan
Yatsuda, Hiromi
Liu, Szu-Heng
Liu, Shih-Jen
Kogai, Takashi
Kuo, Chen-Yen
Wang, Robert Y. L.
A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)
title A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)
title_full A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)
title_fullStr A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)
title_full_unstemmed A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)
title_short A Novel Rapid Test to Detect Anti-SARS-CoV-2 N Protein IgG Based on Shear Horizontal Surface Acoustic Wave (SH-SAW)
title_sort novel rapid test to detect anti-sars-cov-2 n protein igg based on shear horizontal surface acoustic wave (sh-saw)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534600/
https://www.ncbi.nlm.nih.gov/pubmed/34679536
http://dx.doi.org/10.3390/diagnostics11101838
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