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The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey

Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. By single-cell RNA-seque...

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Autores principales: Duan, Kui, Si, Chen-Yang, Zhao, Shu-Mei, Ai, Zong-Yong, Niu, Bao-Hua, Yin, Yu, Xiang, Li-Feng, Ding, Hao, Zheng, Yun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534818/
https://www.ncbi.nlm.nih.gov/pubmed/34685690
http://dx.doi.org/10.3390/cells10102710
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author Duan, Kui
Si, Chen-Yang
Zhao, Shu-Mei
Ai, Zong-Yong
Niu, Bao-Hua
Yin, Yu
Xiang, Li-Feng
Ding, Hao
Zheng, Yun
author_facet Duan, Kui
Si, Chen-Yang
Zhao, Shu-Mei
Ai, Zong-Yong
Niu, Bao-Hua
Yin, Yu
Xiang, Li-Feng
Ding, Hao
Zheng, Yun
author_sort Duan, Kui
collection PubMed
description Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. By single-cell RNA-sequencing (seq) data of cynomolgus monkeys, we found that LTRs of several ERV families, including MacERV6, MacERV3, MacERV2, MacERVK1, and MacERVK2, were highly expressed in pre-implantation embryo cells including epiblast (EPI), trophectoderm (TrB), and primitive endoderm (PrE), but were depleted in post-implantation. We knocked down MacERV6-LTR1a in cynomolgus monkeys with a short hairpin RNA (shRNA) strategy to examine the potential function of MacERV6-LTR1a in the early development of monkey embryos. The silence of MacERV6-LTR1a mainly postpones the differentiation of TrB, EPI, and PrE cells in embryos at day 7 compared to control. Moreover, we confirmed MacERV6-LTR1a could recruit Estrogen Related Receptor Beta (ESRRB), which plays an important role in the maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF and Wnt signaling pathways. In summary, these results suggest that MacERV6-LTR1a is involved in gene regulation of the pre-implantation embryo of the cynomolgus monkeys.
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spelling pubmed-85348182021-10-23 The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey Duan, Kui Si, Chen-Yang Zhao, Shu-Mei Ai, Zong-Yong Niu, Bao-Hua Yin, Yu Xiang, Li-Feng Ding, Hao Zheng, Yun Cells Article Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. By single-cell RNA-sequencing (seq) data of cynomolgus monkeys, we found that LTRs of several ERV families, including MacERV6, MacERV3, MacERV2, MacERVK1, and MacERVK2, were highly expressed in pre-implantation embryo cells including epiblast (EPI), trophectoderm (TrB), and primitive endoderm (PrE), but were depleted in post-implantation. We knocked down MacERV6-LTR1a in cynomolgus monkeys with a short hairpin RNA (shRNA) strategy to examine the potential function of MacERV6-LTR1a in the early development of monkey embryos. The silence of MacERV6-LTR1a mainly postpones the differentiation of TrB, EPI, and PrE cells in embryos at day 7 compared to control. Moreover, we confirmed MacERV6-LTR1a could recruit Estrogen Related Receptor Beta (ESRRB), which plays an important role in the maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF and Wnt signaling pathways. In summary, these results suggest that MacERV6-LTR1a is involved in gene regulation of the pre-implantation embryo of the cynomolgus monkeys. MDPI 2021-10-09 /pmc/articles/PMC8534818/ /pubmed/34685690 http://dx.doi.org/10.3390/cells10102710 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Duan, Kui
Si, Chen-Yang
Zhao, Shu-Mei
Ai, Zong-Yong
Niu, Bao-Hua
Yin, Yu
Xiang, Li-Feng
Ding, Hao
Zheng, Yun
The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
title The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
title_full The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
title_fullStr The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
title_full_unstemmed The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
title_short The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
title_sort long terminal repeats of erv6 are activated in pre-implantation embryos of cynomolgus monkey
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534818/
https://www.ncbi.nlm.nih.gov/pubmed/34685690
http://dx.doi.org/10.3390/cells10102710
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