Cargando…
The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey
Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. By single-cell RNA-seque...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534818/ https://www.ncbi.nlm.nih.gov/pubmed/34685690 http://dx.doi.org/10.3390/cells10102710 |
_version_ | 1784587635451232256 |
---|---|
author | Duan, Kui Si, Chen-Yang Zhao, Shu-Mei Ai, Zong-Yong Niu, Bao-Hua Yin, Yu Xiang, Li-Feng Ding, Hao Zheng, Yun |
author_facet | Duan, Kui Si, Chen-Yang Zhao, Shu-Mei Ai, Zong-Yong Niu, Bao-Hua Yin, Yu Xiang, Li-Feng Ding, Hao Zheng, Yun |
author_sort | Duan, Kui |
collection | PubMed |
description | Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. By single-cell RNA-sequencing (seq) data of cynomolgus monkeys, we found that LTRs of several ERV families, including MacERV6, MacERV3, MacERV2, MacERVK1, and MacERVK2, were highly expressed in pre-implantation embryo cells including epiblast (EPI), trophectoderm (TrB), and primitive endoderm (PrE), but were depleted in post-implantation. We knocked down MacERV6-LTR1a in cynomolgus monkeys with a short hairpin RNA (shRNA) strategy to examine the potential function of MacERV6-LTR1a in the early development of monkey embryos. The silence of MacERV6-LTR1a mainly postpones the differentiation of TrB, EPI, and PrE cells in embryos at day 7 compared to control. Moreover, we confirmed MacERV6-LTR1a could recruit Estrogen Related Receptor Beta (ESRRB), which plays an important role in the maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF and Wnt signaling pathways. In summary, these results suggest that MacERV6-LTR1a is involved in gene regulation of the pre-implantation embryo of the cynomolgus monkeys. |
format | Online Article Text |
id | pubmed-8534818 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85348182021-10-23 The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey Duan, Kui Si, Chen-Yang Zhao, Shu-Mei Ai, Zong-Yong Niu, Bao-Hua Yin, Yu Xiang, Li-Feng Ding, Hao Zheng, Yun Cells Article Precise gene regulation is critical during embryo development. Long terminal repeat elements (LTRs) of endogenous retroviruses (ERVs) are dynamically expressed in blastocysts of mammalian embryos. However, the expression pattern of LTRs in monkey blastocyst is still unknown. By single-cell RNA-sequencing (seq) data of cynomolgus monkeys, we found that LTRs of several ERV families, including MacERV6, MacERV3, MacERV2, MacERVK1, and MacERVK2, were highly expressed in pre-implantation embryo cells including epiblast (EPI), trophectoderm (TrB), and primitive endoderm (PrE), but were depleted in post-implantation. We knocked down MacERV6-LTR1a in cynomolgus monkeys with a short hairpin RNA (shRNA) strategy to examine the potential function of MacERV6-LTR1a in the early development of monkey embryos. The silence of MacERV6-LTR1a mainly postpones the differentiation of TrB, EPI, and PrE cells in embryos at day 7 compared to control. Moreover, we confirmed MacERV6-LTR1a could recruit Estrogen Related Receptor Beta (ESRRB), which plays an important role in the maintenance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF and Wnt signaling pathways. In summary, these results suggest that MacERV6-LTR1a is involved in gene regulation of the pre-implantation embryo of the cynomolgus monkeys. MDPI 2021-10-09 /pmc/articles/PMC8534818/ /pubmed/34685690 http://dx.doi.org/10.3390/cells10102710 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Duan, Kui Si, Chen-Yang Zhao, Shu-Mei Ai, Zong-Yong Niu, Bao-Hua Yin, Yu Xiang, Li-Feng Ding, Hao Zheng, Yun The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey |
title | The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey |
title_full | The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey |
title_fullStr | The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey |
title_full_unstemmed | The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey |
title_short | The Long Terminal Repeats of ERV6 Are Activated in Pre-Implantation Embryos of Cynomolgus Monkey |
title_sort | long terminal repeats of erv6 are activated in pre-implantation embryos of cynomolgus monkey |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534818/ https://www.ncbi.nlm.nih.gov/pubmed/34685690 http://dx.doi.org/10.3390/cells10102710 |
work_keys_str_mv | AT duankui thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT sichenyang thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT zhaoshumei thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT aizongyong thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT niubaohua thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT yinyu thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT xianglifeng thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT dinghao thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT zhengyun thelongterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT duankui longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT sichenyang longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT zhaoshumei longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT aizongyong longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT niubaohua longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT yinyu longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT xianglifeng longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT dinghao longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey AT zhengyun longterminalrepeatsoferv6areactivatedinpreimplantationembryosofcynomolgusmonkey |