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Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells

Tebuconazole (TEB), a triazole fungicide, is frequently applied to agriculture for the increase of food production. Although TEB causes liver toxicity, its effects on cellular lipid accumulation are rarely investigated. Therefore, this study aimed to study the effects of TEB on lipid metabolism and...

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Autores principales: Kwon, Hyuk-Cheol, Kim, Do-Hyun, Jeong, Chang-Hee, Kim, Yea-Ji, Han, Jong-Hyun, Lim, Su-Jin, Shin, Dong-Min, Kim, Dong-Wook, Han, Sung-Gu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534864/
https://www.ncbi.nlm.nih.gov/pubmed/34681291
http://dx.doi.org/10.3390/foods10102242
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author Kwon, Hyuk-Cheol
Kim, Do-Hyun
Jeong, Chang-Hee
Kim, Yea-Ji
Han, Jong-Hyun
Lim, Su-Jin
Shin, Dong-Min
Kim, Dong-Wook
Han, Sung-Gu
author_facet Kwon, Hyuk-Cheol
Kim, Do-Hyun
Jeong, Chang-Hee
Kim, Yea-Ji
Han, Jong-Hyun
Lim, Su-Jin
Shin, Dong-Min
Kim, Dong-Wook
Han, Sung-Gu
author_sort Kwon, Hyuk-Cheol
collection PubMed
description Tebuconazole (TEB), a triazole fungicide, is frequently applied to agriculture for the increase of food production. Although TEB causes liver toxicity, its effects on cellular lipid accumulation are rarely investigated. Therefore, this study aimed to study the effects of TEB on lipid metabolism and accumulation in HepG2 cells. HepG2 cells were exposed to 0–320 µM TEB for 1–24 h. TEB (20–80 µM, 24 h)-treated cells showed lipid accumulation. Further, TEB (20–80 µM, 1–12 h) increased the nuclear translocation of peroxisome proliferator-activated receptors and the expression of lipid uptake and oxidation-related markers such as cluster of differentiation 36, fatty acid transport protein (FATP) 2, FATP5, and carnitine palmitoyltransferase 1. Oxidative stress levels in TEB-treated cells (20–80 µM, 24 h) were higher, compared to those in the control. TEB (20–80 µM, 24 h) also induced the loss of mitochondrial membrane potential and lower levels of microsomal triglyceride transfer protein in the cells. Thus, TEB can induce lipid accumulation by altering the expression of lipid-metabolizing molecules and can therefore impair lipid metabolism. Our data suggest that human exposure to TEB may be a risk factor for non-alcoholic fatty liver disease.
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spelling pubmed-85348642021-10-23 Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells Kwon, Hyuk-Cheol Kim, Do-Hyun Jeong, Chang-Hee Kim, Yea-Ji Han, Jong-Hyun Lim, Su-Jin Shin, Dong-Min Kim, Dong-Wook Han, Sung-Gu Foods Article Tebuconazole (TEB), a triazole fungicide, is frequently applied to agriculture for the increase of food production. Although TEB causes liver toxicity, its effects on cellular lipid accumulation are rarely investigated. Therefore, this study aimed to study the effects of TEB on lipid metabolism and accumulation in HepG2 cells. HepG2 cells were exposed to 0–320 µM TEB for 1–24 h. TEB (20–80 µM, 24 h)-treated cells showed lipid accumulation. Further, TEB (20–80 µM, 1–12 h) increased the nuclear translocation of peroxisome proliferator-activated receptors and the expression of lipid uptake and oxidation-related markers such as cluster of differentiation 36, fatty acid transport protein (FATP) 2, FATP5, and carnitine palmitoyltransferase 1. Oxidative stress levels in TEB-treated cells (20–80 µM, 24 h) were higher, compared to those in the control. TEB (20–80 µM, 24 h) also induced the loss of mitochondrial membrane potential and lower levels of microsomal triglyceride transfer protein in the cells. Thus, TEB can induce lipid accumulation by altering the expression of lipid-metabolizing molecules and can therefore impair lipid metabolism. Our data suggest that human exposure to TEB may be a risk factor for non-alcoholic fatty liver disease. MDPI 2021-09-22 /pmc/articles/PMC8534864/ /pubmed/34681291 http://dx.doi.org/10.3390/foods10102242 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kwon, Hyuk-Cheol
Kim, Do-Hyun
Jeong, Chang-Hee
Kim, Yea-Ji
Han, Jong-Hyun
Lim, Su-Jin
Shin, Dong-Min
Kim, Dong-Wook
Han, Sung-Gu
Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells
title Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells
title_full Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells
title_fullStr Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells
title_full_unstemmed Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells
title_short Tebuconazole Fungicide Induces Lipid Accumulation and Oxidative Stress in HepG2 Cells
title_sort tebuconazole fungicide induces lipid accumulation and oxidative stress in hepg2 cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8534864/
https://www.ncbi.nlm.nih.gov/pubmed/34681291
http://dx.doi.org/10.3390/foods10102242
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