Cargando…

Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study

Defined as an index of platelet size heterogeneity, the platelet distribution width (PDW) is still a poorly characterized marker of platelet function in (sub)clinical disease. We presently validated PDW as a marker of P-selectin dependent platelet activation in the Moli-family cohort. Platelet-bound...

Descripción completa

Detalles Bibliográficos
Autores principales: Izzi, Benedetta, Gialluisi, Alessandro, Gianfagna, Francesco, Orlandi, Sabatino, De Curtis, Amalia, Magnacca, Sara, Costanzo, Simona, Di Castelnuovo, Augusto, Donati, Maria Benedetta, de Gaetano, Giovanni, Hoylaerts, Marc F., Cerletti, Chiara, Iacoviello, Licia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535046/
https://www.ncbi.nlm.nih.gov/pubmed/34685717
http://dx.doi.org/10.3390/cells10102737
_version_ 1784587682992619520
author Izzi, Benedetta
Gialluisi, Alessandro
Gianfagna, Francesco
Orlandi, Sabatino
De Curtis, Amalia
Magnacca, Sara
Costanzo, Simona
Di Castelnuovo, Augusto
Donati, Maria Benedetta
de Gaetano, Giovanni
Hoylaerts, Marc F.
Cerletti, Chiara
Iacoviello, Licia
author_facet Izzi, Benedetta
Gialluisi, Alessandro
Gianfagna, Francesco
Orlandi, Sabatino
De Curtis, Amalia
Magnacca, Sara
Costanzo, Simona
Di Castelnuovo, Augusto
Donati, Maria Benedetta
de Gaetano, Giovanni
Hoylaerts, Marc F.
Cerletti, Chiara
Iacoviello, Licia
author_sort Izzi, Benedetta
collection PubMed
description Defined as an index of platelet size heterogeneity, the platelet distribution width (PDW) is still a poorly characterized marker of platelet function in (sub)clinical disease. We presently validated PDW as a marker of P-selectin dependent platelet activation in the Moli-family cohort. Platelet-bound P-selectin and platelet/leukocyte mixed aggregates were measured by flow cytometry in freshly collected venous blood, both before and after in vitro platelet activation, and coagulation time was assessed in unstimulated and LPS- or TNFα-stimulated whole blood. Closure Times (CT) were measured in a Platelet Function Analyzer (PFA)-100. Multivariable linear mixed effect regression models (with age, sex and platelet count as fixed and family structure as random effect) revealed PDW to be negatively associated with platelet P-selectin, platelet/leukocyte aggregates and von Willebrand factor (VWF), and positively with PFA-100 CT, and LPS- and TNF-α-stimulated coagulation times. With the exception of VWF, all relationships were sex-independent. In contrast, no association was found between mean platelet volume (MPV) and these variables. PDW seems a simple, useful marker of ex vivo and in vitro P-selectin dependent platelet activation. Investigations of larger cohorts will define the usefulness of PDW as a risk predictor of thrombo-inflammatory conditions where activated platelets play a contributing role.
format Online
Article
Text
id pubmed-8535046
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85350462021-10-23 Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study Izzi, Benedetta Gialluisi, Alessandro Gianfagna, Francesco Orlandi, Sabatino De Curtis, Amalia Magnacca, Sara Costanzo, Simona Di Castelnuovo, Augusto Donati, Maria Benedetta de Gaetano, Giovanni Hoylaerts, Marc F. Cerletti, Chiara Iacoviello, Licia Cells Article Defined as an index of platelet size heterogeneity, the platelet distribution width (PDW) is still a poorly characterized marker of platelet function in (sub)clinical disease. We presently validated PDW as a marker of P-selectin dependent platelet activation in the Moli-family cohort. Platelet-bound P-selectin and platelet/leukocyte mixed aggregates were measured by flow cytometry in freshly collected venous blood, both before and after in vitro platelet activation, and coagulation time was assessed in unstimulated and LPS- or TNFα-stimulated whole blood. Closure Times (CT) were measured in a Platelet Function Analyzer (PFA)-100. Multivariable linear mixed effect regression models (with age, sex and platelet count as fixed and family structure as random effect) revealed PDW to be negatively associated with platelet P-selectin, platelet/leukocyte aggregates and von Willebrand factor (VWF), and positively with PFA-100 CT, and LPS- and TNF-α-stimulated coagulation times. With the exception of VWF, all relationships were sex-independent. In contrast, no association was found between mean platelet volume (MPV) and these variables. PDW seems a simple, useful marker of ex vivo and in vitro P-selectin dependent platelet activation. Investigations of larger cohorts will define the usefulness of PDW as a risk predictor of thrombo-inflammatory conditions where activated platelets play a contributing role. MDPI 2021-10-13 /pmc/articles/PMC8535046/ /pubmed/34685717 http://dx.doi.org/10.3390/cells10102737 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Izzi, Benedetta
Gialluisi, Alessandro
Gianfagna, Francesco
Orlandi, Sabatino
De Curtis, Amalia
Magnacca, Sara
Costanzo, Simona
Di Castelnuovo, Augusto
Donati, Maria Benedetta
de Gaetano, Giovanni
Hoylaerts, Marc F.
Cerletti, Chiara
Iacoviello, Licia
Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study
title Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study
title_full Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study
title_fullStr Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study
title_full_unstemmed Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study
title_short Platelet Distribution Width Is Associated with P-Selectin Dependent Platelet Function: Results from the Moli-Family Cohort Study
title_sort platelet distribution width is associated with p-selectin dependent platelet function: results from the moli-family cohort study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535046/
https://www.ncbi.nlm.nih.gov/pubmed/34685717
http://dx.doi.org/10.3390/cells10102737
work_keys_str_mv AT izzibenedetta plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT gialluisialessandro plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT gianfagnafrancesco plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT orlandisabatino plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT decurtisamalia plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT magnaccasara plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT costanzosimona plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT dicastelnuovoaugusto plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT donatimariabenedetta plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT degaetanogiovanni plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT hoylaertsmarcf plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT cerlettichiara plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT iacoviellolicia plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy
AT plateletdistributionwidthisassociatedwithpselectindependentplateletfunctionresultsfromthemolifamilycohortstudy