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Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
OBJECTIVE: The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated th...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535375/ https://www.ncbi.nlm.nih.gov/pubmed/34679081 http://dx.doi.org/10.1371/journal.pone.0254966 |
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author | Shinohara, Yuta Washio, Jumpei Kobayashi, Yuri Abiko, Yuki Sasaki, Keiichi Takahashi, Nobuhiro |
author_facet | Shinohara, Yuta Washio, Jumpei Kobayashi, Yuri Abiko, Yuki Sasaki, Keiichi Takahashi, Nobuhiro |
author_sort | Shinohara, Yuta |
collection | PubMed |
description | OBJECTIVE: The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. METHODS: The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. RESULTS: Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. CONCLUSIONS: Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation. |
format | Online Article Text |
id | pubmed-8535375 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-85353752021-10-23 Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions Shinohara, Yuta Washio, Jumpei Kobayashi, Yuri Abiko, Yuki Sasaki, Keiichi Takahashi, Nobuhiro PLoS One Research Article OBJECTIVE: The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. METHODS: The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. RESULTS: Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. CONCLUSIONS: Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation. Public Library of Science 2021-10-22 /pmc/articles/PMC8535375/ /pubmed/34679081 http://dx.doi.org/10.1371/journal.pone.0254966 Text en © 2021 Shinohara et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shinohara, Yuta Washio, Jumpei Kobayashi, Yuri Abiko, Yuki Sasaki, Keiichi Takahashi, Nobuhiro Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
title | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
title_full | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
title_fullStr | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
title_full_unstemmed | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
title_short | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
title_sort | hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535375/ https://www.ncbi.nlm.nih.gov/pubmed/34679081 http://dx.doi.org/10.1371/journal.pone.0254966 |
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