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Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia
Acute Lymphoblastic Leukemia (ALL) is a neoplasm of the hematopoietic system defined as a clonal expansion of an abnormal lymphoid precursor cell. It mostly affects children under five years of age and is the most common tumor to afflict pediatric patients. The expression of the human telomerase gen...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535500/ https://www.ncbi.nlm.nih.gov/pubmed/34681025 http://dx.doi.org/10.3390/genes12101632 |
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author | Nogueira, Beatriz Maria Dias Pantoja, Laudreísa da Costa da Silva, Emerson Lucena Mello Júnior, Fernando Augusto Rodrigues Teixeira, Eliel Barbosa Wanderley, Alayde Vieira Maués, Jersey Heitor da Silva Filho, Manoel Odorico de Moraes de Moraes, Maria Elisabete Amaral Montenegro, Raquel Carvalho Khayat, André Salim Moreira-Nunes, Caroline Aquino |
author_facet | Nogueira, Beatriz Maria Dias Pantoja, Laudreísa da Costa da Silva, Emerson Lucena Mello Júnior, Fernando Augusto Rodrigues Teixeira, Eliel Barbosa Wanderley, Alayde Vieira Maués, Jersey Heitor da Silva Filho, Manoel Odorico de Moraes de Moraes, Maria Elisabete Amaral Montenegro, Raquel Carvalho Khayat, André Salim Moreira-Nunes, Caroline Aquino |
author_sort | Nogueira, Beatriz Maria Dias |
collection | PubMed |
description | Acute Lymphoblastic Leukemia (ALL) is a neoplasm of the hematopoietic system defined as a clonal expansion of an abnormal lymphoid precursor cell. It mostly affects children under five years of age and is the most common tumor to afflict pediatric patients. The expression of the human telomerase gene (hTERT) in patients with ALL has been studied as a biomarker and could become a new therapeutic target. We evaluate the role of hTERT gene expression in ALL pediatric patients, through quantitative real-time PCR technique, and the possible correlation between hTERT expression and clinical variables: gender, age, white blood cells (WBC), gene fusions, and immunophenotyping. The analysis between healthy controls and ALL patients (N = 244) was statistically significant (p < 0.001), demonstrating hTERT overexpression in these patients. In comparison with the usual set of clinical variables, the data were not statistically significant (p > 0.05), indicating that hTERT is equally overexpressed among patients regardless of gender, age, gene fusions, and immunophenotyping. Moreover, patients who presented a higher hTERT expression level had a significant (p < 0.0001) lower overall survival rate. In summary, hTERT expression emerges as an important molecular pathway in leukemogenesis regardless patient’s clinical variables, thus, the data here presented pointed it as a valuable biomarker in pediatric acute lymphoblastic leukemia and a promising target for new therapeutic and prognostic measures. |
format | Online Article Text |
id | pubmed-8535500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85355002021-10-23 Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia Nogueira, Beatriz Maria Dias Pantoja, Laudreísa da Costa da Silva, Emerson Lucena Mello Júnior, Fernando Augusto Rodrigues Teixeira, Eliel Barbosa Wanderley, Alayde Vieira Maués, Jersey Heitor da Silva Filho, Manoel Odorico de Moraes de Moraes, Maria Elisabete Amaral Montenegro, Raquel Carvalho Khayat, André Salim Moreira-Nunes, Caroline Aquino Genes (Basel) Article Acute Lymphoblastic Leukemia (ALL) is a neoplasm of the hematopoietic system defined as a clonal expansion of an abnormal lymphoid precursor cell. It mostly affects children under five years of age and is the most common tumor to afflict pediatric patients. The expression of the human telomerase gene (hTERT) in patients with ALL has been studied as a biomarker and could become a new therapeutic target. We evaluate the role of hTERT gene expression in ALL pediatric patients, through quantitative real-time PCR technique, and the possible correlation between hTERT expression and clinical variables: gender, age, white blood cells (WBC), gene fusions, and immunophenotyping. The analysis between healthy controls and ALL patients (N = 244) was statistically significant (p < 0.001), demonstrating hTERT overexpression in these patients. In comparison with the usual set of clinical variables, the data were not statistically significant (p > 0.05), indicating that hTERT is equally overexpressed among patients regardless of gender, age, gene fusions, and immunophenotyping. Moreover, patients who presented a higher hTERT expression level had a significant (p < 0.0001) lower overall survival rate. In summary, hTERT expression emerges as an important molecular pathway in leukemogenesis regardless patient’s clinical variables, thus, the data here presented pointed it as a valuable biomarker in pediatric acute lymphoblastic leukemia and a promising target for new therapeutic and prognostic measures. MDPI 2021-10-17 /pmc/articles/PMC8535500/ /pubmed/34681025 http://dx.doi.org/10.3390/genes12101632 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Nogueira, Beatriz Maria Dias Pantoja, Laudreísa da Costa da Silva, Emerson Lucena Mello Júnior, Fernando Augusto Rodrigues Teixeira, Eliel Barbosa Wanderley, Alayde Vieira Maués, Jersey Heitor da Silva Filho, Manoel Odorico de Moraes de Moraes, Maria Elisabete Amaral Montenegro, Raquel Carvalho Khayat, André Salim Moreira-Nunes, Caroline Aquino Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia |
title | Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia |
title_full | Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia |
title_fullStr | Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia |
title_full_unstemmed | Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia |
title_short | Telomerase (hTERT) Overexpression Reveals a Promising Prognostic Biomarker and Therapeutical Target in Different Clinical Subtypes of Pediatric Acute Lymphoblastic Leukaemia |
title_sort | telomerase (htert) overexpression reveals a promising prognostic biomarker and therapeutical target in different clinical subtypes of pediatric acute lymphoblastic leukaemia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535500/ https://www.ncbi.nlm.nih.gov/pubmed/34681025 http://dx.doi.org/10.3390/genes12101632 |
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