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The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database

Campylobacter jejuni (C. jejuni) is responsible for 80% of human campylobacteriosis and is the leading cause of gastroenteritis globally. The relevant public health risks of C. jejuni are caused by particular virulence genes encompassing its virulome. We analyzed 40,371 publicly available genomes of...

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Autores principales: Panzenhagen, Pedro, Portes, Ana Beatriz, dos Santos, Anamaria M. P., Duque, Sheila da Silva, Conte Junior, Carlos Adam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535712/
https://www.ncbi.nlm.nih.gov/pubmed/34680933
http://dx.doi.org/10.3390/genes12101538
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author Panzenhagen, Pedro
Portes, Ana Beatriz
dos Santos, Anamaria M. P.
Duque, Sheila da Silva
Conte Junior, Carlos Adam
author_facet Panzenhagen, Pedro
Portes, Ana Beatriz
dos Santos, Anamaria M. P.
Duque, Sheila da Silva
Conte Junior, Carlos Adam
author_sort Panzenhagen, Pedro
collection PubMed
description Campylobacter jejuni (C. jejuni) is responsible for 80% of human campylobacteriosis and is the leading cause of gastroenteritis globally. The relevant public health risks of C. jejuni are caused by particular virulence genes encompassing its virulome. We analyzed 40,371 publicly available genomes of C. jejuni deposited in the NCBI Pathogen Detection Database, combining their epidemiologic metadata with an in silico bioinformatics analysis to increase our current comprehension of their virulome from a global perspective. The collection presented a virulome composed of 126 identified virulence factors that were grouped in three clusters representing the accessory, the softcore, and the essential core genes according to their prevalence within the genomes. The multilocus sequence type distribution in the genomes was also investigated. An unexpected low prevalence of the full-length flagellin flaA and flaB locus of C. jejuni genomes was revealed, and an essential core virulence gene repertoire prevalent in more than 99.99% of genomes was identified. Altogether, this is a pioneer study regarding Campylobacter jejuni that has compiled a significant amount of data about the Multilocus Sequence Type and virulence factors concerning their global prevalence and distribution over this database.
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spelling pubmed-85357122021-10-23 The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database Panzenhagen, Pedro Portes, Ana Beatriz dos Santos, Anamaria M. P. Duque, Sheila da Silva Conte Junior, Carlos Adam Genes (Basel) Article Campylobacter jejuni (C. jejuni) is responsible for 80% of human campylobacteriosis and is the leading cause of gastroenteritis globally. The relevant public health risks of C. jejuni are caused by particular virulence genes encompassing its virulome. We analyzed 40,371 publicly available genomes of C. jejuni deposited in the NCBI Pathogen Detection Database, combining their epidemiologic metadata with an in silico bioinformatics analysis to increase our current comprehension of their virulome from a global perspective. The collection presented a virulome composed of 126 identified virulence factors that were grouped in three clusters representing the accessory, the softcore, and the essential core genes according to their prevalence within the genomes. The multilocus sequence type distribution in the genomes was also investigated. An unexpected low prevalence of the full-length flagellin flaA and flaB locus of C. jejuni genomes was revealed, and an essential core virulence gene repertoire prevalent in more than 99.99% of genomes was identified. Altogether, this is a pioneer study regarding Campylobacter jejuni that has compiled a significant amount of data about the Multilocus Sequence Type and virulence factors concerning their global prevalence and distribution over this database. MDPI 2021-09-28 /pmc/articles/PMC8535712/ /pubmed/34680933 http://dx.doi.org/10.3390/genes12101538 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panzenhagen, Pedro
Portes, Ana Beatriz
dos Santos, Anamaria M. P.
Duque, Sheila da Silva
Conte Junior, Carlos Adam
The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database
title The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database
title_full The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database
title_fullStr The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database
title_full_unstemmed The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database
title_short The Distribution of Campylobacter jejuni Virulence Genes in Genomes Worldwide Derived from the NCBI Pathogen Detection Database
title_sort distribution of campylobacter jejuni virulence genes in genomes worldwide derived from the ncbi pathogen detection database
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535712/
https://www.ncbi.nlm.nih.gov/pubmed/34680933
http://dx.doi.org/10.3390/genes12101538
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