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Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains
Rice is one of the most important food crops in the world and is of vital importance to many countries. Various diseases caused by fungi, bacteria and viruses constantly threaten rice plants and cause yield losses. Bacterial leaf streak disease (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc)...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535723/ https://www.ncbi.nlm.nih.gov/pubmed/34680982 http://dx.doi.org/10.3390/genes12101587 |
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author | Thianthavon, Tripop Aesomnuk, Wanchana Pitaloka, Mutiara K. Sattayachiti, Wannapa Sonsom, Yupin Nubankoh, Phakchana Malichan, Srihunsa Riangwong, Kanamon Ruanjaichon, Vinitchan Toojinda, Theerayut Wanchana, Samart Arikit, Siwaret |
author_facet | Thianthavon, Tripop Aesomnuk, Wanchana Pitaloka, Mutiara K. Sattayachiti, Wannapa Sonsom, Yupin Nubankoh, Phakchana Malichan, Srihunsa Riangwong, Kanamon Ruanjaichon, Vinitchan Toojinda, Theerayut Wanchana, Samart Arikit, Siwaret |
author_sort | Thianthavon, Tripop |
collection | PubMed |
description | Rice is one of the most important food crops in the world and is of vital importance to many countries. Various diseases caused by fungi, bacteria and viruses constantly threaten rice plants and cause yield losses. Bacterial leaf streak disease (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most devastating rice diseases. However, most modern rice varieties are susceptible to BLS. In this study, we applied the QTL-seq approach using an F(2) population derived from the cross between IR62266 and Homcholasit (HSC) to rapidly identify the quantitative trait loci (QTL) that confers resistance to BLS caused by a Thai Xoc isolate, SP7-5. The results showed that a single genomic region at the beginning of chromosome 5 was highly associated with resistance to BLS. The gene xa5 was considered a potential candidate gene in this region since most associated single nucleotide polymorphisms (SNPs) were within this gene. A Kompetitive Allele-Specific PCR (KASP) marker was developed based on two consecutive functional SNPs in xa5 and validated in six F(2) populations inoculated with another Thai Xoc isolate, 2NY2-2. The phenotypic variance explained by this marker (PVE) ranged from 59.04% to 70.84% in the six populations. These findings indicate that xa5 is a viable candidate gene for BLS resistance and may help in breeding programs for BLS resistance. |
format | Online Article Text |
id | pubmed-8535723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85357232021-10-23 Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains Thianthavon, Tripop Aesomnuk, Wanchana Pitaloka, Mutiara K. Sattayachiti, Wannapa Sonsom, Yupin Nubankoh, Phakchana Malichan, Srihunsa Riangwong, Kanamon Ruanjaichon, Vinitchan Toojinda, Theerayut Wanchana, Samart Arikit, Siwaret Genes (Basel) Article Rice is one of the most important food crops in the world and is of vital importance to many countries. Various diseases caused by fungi, bacteria and viruses constantly threaten rice plants and cause yield losses. Bacterial leaf streak disease (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc) is one of the most devastating rice diseases. However, most modern rice varieties are susceptible to BLS. In this study, we applied the QTL-seq approach using an F(2) population derived from the cross between IR62266 and Homcholasit (HSC) to rapidly identify the quantitative trait loci (QTL) that confers resistance to BLS caused by a Thai Xoc isolate, SP7-5. The results showed that a single genomic region at the beginning of chromosome 5 was highly associated with resistance to BLS. The gene xa5 was considered a potential candidate gene in this region since most associated single nucleotide polymorphisms (SNPs) were within this gene. A Kompetitive Allele-Specific PCR (KASP) marker was developed based on two consecutive functional SNPs in xa5 and validated in six F(2) populations inoculated with another Thai Xoc isolate, 2NY2-2. The phenotypic variance explained by this marker (PVE) ranged from 59.04% to 70.84% in the six populations. These findings indicate that xa5 is a viable candidate gene for BLS resistance and may help in breeding programs for BLS resistance. MDPI 2021-10-09 /pmc/articles/PMC8535723/ /pubmed/34680982 http://dx.doi.org/10.3390/genes12101587 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Thianthavon, Tripop Aesomnuk, Wanchana Pitaloka, Mutiara K. Sattayachiti, Wannapa Sonsom, Yupin Nubankoh, Phakchana Malichan, Srihunsa Riangwong, Kanamon Ruanjaichon, Vinitchan Toojinda, Theerayut Wanchana, Samart Arikit, Siwaret Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains |
title | Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains |
title_full | Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains |
title_fullStr | Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains |
title_full_unstemmed | Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains |
title_short | Identification and Validation of a QTL for Bacterial Leaf Streak Resistance in Rice (Oryza sativa L.) against Thai Xoc Strains |
title_sort | identification and validation of a qtl for bacterial leaf streak resistance in rice (oryza sativa l.) against thai xoc strains |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535723/ https://www.ncbi.nlm.nih.gov/pubmed/34680982 http://dx.doi.org/10.3390/genes12101587 |
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