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Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis)
The evolution of herbicide-resistant weed species is a serious threat for weed control. Therefore, we need an improved understanding of how gene regulation confers herbicide resistance in order to slow the evolution of resistance. The present study analyzed differentially expressed genes after glyph...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535903/ https://www.ncbi.nlm.nih.gov/pubmed/34681011 http://dx.doi.org/10.3390/genes12101616 |
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author | Yang, Yongil Gardner, Cory Gupta, Pallavi Peng, Yanhui Piasecki, Cristiano Millwood, Reginald J. Ahn, Tae-Hyuk Stewart, C. Neal |
author_facet | Yang, Yongil Gardner, Cory Gupta, Pallavi Peng, Yanhui Piasecki, Cristiano Millwood, Reginald J. Ahn, Tae-Hyuk Stewart, C. Neal |
author_sort | Yang, Yongil |
collection | PubMed |
description | The evolution of herbicide-resistant weed species is a serious threat for weed control. Therefore, we need an improved understanding of how gene regulation confers herbicide resistance in order to slow the evolution of resistance. The present study analyzed differentially expressed genes after glyphosate treatment on a glyphosate-resistant Tennessee ecotype (TNR) of horseweed (Conyza canadensis), compared to a susceptible biotype (TNS). A read size of 100.2 M was sequenced on the Illumina platform and subjected to de novo assembly, resulting in 77,072 gene-level contigs, of which 32,493 were uniquely annotated by a BlastX alignment of protein sequence similarity. The most differentially expressed genes were enriched in the gene ontology (GO) term of the transmembrane transport protein. In addition, fifteen upregulated genes were identified in TNR after glyphosate treatment but were not detected in TNS. Ten of these upregulated genes were transmembrane transporter or kinase receptor proteins. Therefore, a combination of changes in gene expression among transmembrane receptor and kinase receptor proteins may be important for endowing non-target-site glyphosate-resistant C. canadensis. |
format | Online Article Text |
id | pubmed-8535903 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85359032021-10-23 Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis) Yang, Yongil Gardner, Cory Gupta, Pallavi Peng, Yanhui Piasecki, Cristiano Millwood, Reginald J. Ahn, Tae-Hyuk Stewart, C. Neal Genes (Basel) Article The evolution of herbicide-resistant weed species is a serious threat for weed control. Therefore, we need an improved understanding of how gene regulation confers herbicide resistance in order to slow the evolution of resistance. The present study analyzed differentially expressed genes after glyphosate treatment on a glyphosate-resistant Tennessee ecotype (TNR) of horseweed (Conyza canadensis), compared to a susceptible biotype (TNS). A read size of 100.2 M was sequenced on the Illumina platform and subjected to de novo assembly, resulting in 77,072 gene-level contigs, of which 32,493 were uniquely annotated by a BlastX alignment of protein sequence similarity. The most differentially expressed genes were enriched in the gene ontology (GO) term of the transmembrane transport protein. In addition, fifteen upregulated genes were identified in TNR after glyphosate treatment but were not detected in TNS. Ten of these upregulated genes were transmembrane transporter or kinase receptor proteins. Therefore, a combination of changes in gene expression among transmembrane receptor and kinase receptor proteins may be important for endowing non-target-site glyphosate-resistant C. canadensis. MDPI 2021-10-14 /pmc/articles/PMC8535903/ /pubmed/34681011 http://dx.doi.org/10.3390/genes12101616 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Yongil Gardner, Cory Gupta, Pallavi Peng, Yanhui Piasecki, Cristiano Millwood, Reginald J. Ahn, Tae-Hyuk Stewart, C. Neal Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis) |
title | Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis) |
title_full | Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis) |
title_fullStr | Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis) |
title_full_unstemmed | Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis) |
title_short | Novel Candidate Genes Differentially Expressed in Glyphosate-Treated Horseweed (Conyza canadensis) |
title_sort | novel candidate genes differentially expressed in glyphosate-treated horseweed (conyza canadensis) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535903/ https://www.ncbi.nlm.nih.gov/pubmed/34681011 http://dx.doi.org/10.3390/genes12101616 |
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