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Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile

Inflammatory factors have been considered to contribute to keratoconus (KC) pathogenesis. This study aims to determine the immune cells subsets and soluble inflammatory factor profile on the ocular surface of KC patients. 32 KC subjects (51 eyes) across different grades of severity and 15 healthy co...

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Autores principales: D’Souza, Sharon, Nair, Archana Padmanabhan, Sahu, Ganesh Ram, Vaidya, Tanuja, Shetty, Rohit, Khamar, Pooja, Mullick, Ritika, Gupta, Sneha, Dickman, Mor M., Nuijts, Rudy M. M. A., Mohan, Rajiv R., Ghosh, Arkasubhra, Sethu, Swaminathan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536707/
https://www.ncbi.nlm.nih.gov/pubmed/34686755
http://dx.doi.org/10.1038/s41598-021-99805-9
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author D’Souza, Sharon
Nair, Archana Padmanabhan
Sahu, Ganesh Ram
Vaidya, Tanuja
Shetty, Rohit
Khamar, Pooja
Mullick, Ritika
Gupta, Sneha
Dickman, Mor M.
Nuijts, Rudy M. M. A.
Mohan, Rajiv R.
Ghosh, Arkasubhra
Sethu, Swaminathan
author_facet D’Souza, Sharon
Nair, Archana Padmanabhan
Sahu, Ganesh Ram
Vaidya, Tanuja
Shetty, Rohit
Khamar, Pooja
Mullick, Ritika
Gupta, Sneha
Dickman, Mor M.
Nuijts, Rudy M. M. A.
Mohan, Rajiv R.
Ghosh, Arkasubhra
Sethu, Swaminathan
author_sort D’Souza, Sharon
collection PubMed
description Inflammatory factors have been considered to contribute to keratoconus (KC) pathogenesis. This study aims to determine the immune cells subsets and soluble inflammatory factor profile on the ocular surface of KC patients. 32 KC subjects (51 eyes) across different grades of severity and 15 healthy controls (23 eyes) were included in the study. Keratometry and pachymetry measurements were recorded. Ocular surface immune cells (collected by ocular surface wash) immunophenotyped using flow cytometry include leukocytes, neutrophils, macrophages, natural killer (NK) cells, pan-T cells, gamma delta T (γδT) cells and NKT cells. Tear fluid collected using Schirmer’s strip was used to measure 50 soluble factors by multiplex ELISA. Proportions of activated neutrophils, NK cells and γδT cells were significantly increased in KC patients. Significantly higher levels of tear fluid IL-1β, IL-6, LIF, IL-17A, TNFα, IFNα/β/γ, EPO, TGFβ1, PDGF-BB, sVCAM, sL-selectin, granzyme-B, perforin, MMP2, sFasL and IgE, along with significantly lower levels of IL-1α and IL-9 were observed in KC patients. Alterations observed in few of the immuno-inflammatory parameters correlated with grades of disease, allergy, eye rubbing and keratometry or pachymetry measurements. The observation implies a distinct immuno-inflammatory component in KC pathogenesis and its potential as an additional therapeutic target in KC management.
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spelling pubmed-85367072021-10-25 Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile D’Souza, Sharon Nair, Archana Padmanabhan Sahu, Ganesh Ram Vaidya, Tanuja Shetty, Rohit Khamar, Pooja Mullick, Ritika Gupta, Sneha Dickman, Mor M. Nuijts, Rudy M. M. A. Mohan, Rajiv R. Ghosh, Arkasubhra Sethu, Swaminathan Sci Rep Article Inflammatory factors have been considered to contribute to keratoconus (KC) pathogenesis. This study aims to determine the immune cells subsets and soluble inflammatory factor profile on the ocular surface of KC patients. 32 KC subjects (51 eyes) across different grades of severity and 15 healthy controls (23 eyes) were included in the study. Keratometry and pachymetry measurements were recorded. Ocular surface immune cells (collected by ocular surface wash) immunophenotyped using flow cytometry include leukocytes, neutrophils, macrophages, natural killer (NK) cells, pan-T cells, gamma delta T (γδT) cells and NKT cells. Tear fluid collected using Schirmer’s strip was used to measure 50 soluble factors by multiplex ELISA. Proportions of activated neutrophils, NK cells and γδT cells were significantly increased in KC patients. Significantly higher levels of tear fluid IL-1β, IL-6, LIF, IL-17A, TNFα, IFNα/β/γ, EPO, TGFβ1, PDGF-BB, sVCAM, sL-selectin, granzyme-B, perforin, MMP2, sFasL and IgE, along with significantly lower levels of IL-1α and IL-9 were observed in KC patients. Alterations observed in few of the immuno-inflammatory parameters correlated with grades of disease, allergy, eye rubbing and keratometry or pachymetry measurements. The observation implies a distinct immuno-inflammatory component in KC pathogenesis and its potential as an additional therapeutic target in KC management. Nature Publishing Group UK 2021-10-22 /pmc/articles/PMC8536707/ /pubmed/34686755 http://dx.doi.org/10.1038/s41598-021-99805-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
D’Souza, Sharon
Nair, Archana Padmanabhan
Sahu, Ganesh Ram
Vaidya, Tanuja
Shetty, Rohit
Khamar, Pooja
Mullick, Ritika
Gupta, Sneha
Dickman, Mor M.
Nuijts, Rudy M. M. A.
Mohan, Rajiv R.
Ghosh, Arkasubhra
Sethu, Swaminathan
Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
title Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
title_full Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
title_fullStr Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
title_full_unstemmed Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
title_short Keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
title_sort keratoconus patients exhibit a distinct ocular surface immune cell and inflammatory profile
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536707/
https://www.ncbi.nlm.nih.gov/pubmed/34686755
http://dx.doi.org/10.1038/s41598-021-99805-9
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