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Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive
Phenotypic diversity in bacterial flagella-induced motility leads to complex collective swimming patterns, appearing as traveling bands with transient locally enhanced cell densities. Traveling bands are known to be a bacterial chemotactic response to self-generated nutrient gradients during growth...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536744/ https://www.ncbi.nlm.nih.gov/pubmed/34745645 http://dx.doi.org/10.1038/s41378-021-00309-3 |
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author | Liu, Yang Lehnert, Thomas Gijs, Martin A. M. |
author_facet | Liu, Yang Lehnert, Thomas Gijs, Martin A. M. |
author_sort | Liu, Yang |
collection | PubMed |
description | Phenotypic diversity in bacterial flagella-induced motility leads to complex collective swimming patterns, appearing as traveling bands with transient locally enhanced cell densities. Traveling bands are known to be a bacterial chemotactic response to self-generated nutrient gradients during growth in resource-limited microenvironments. In this work, we studied different parameters of Escherichia coli (E. coli) collective migration, in particular the quantity of bacteria introduced initially in a microfluidic chip (inoculum size) and their exposure to antibiotics (ampicillin, ciprofloxacin, and gentamicin). We developed a hybrid polymer-glass chip with an intermediate optical adhesive layer featuring the microfluidic channel, enabling high-content imaging of the migration dynamics in a single bacterial layer, i.e., bacteria are confined in a quasi-2D space that is fully observable with a high-magnification microscope objective. On-chip bacterial motility and traveling band analysis was performed based on individual bacterial trajectories by means of custom-developed algorithms. Quantifications of swimming speed, tumble bias and effective diffusion properties allowed the assessment of phenotypic heterogeneity, resulting in variations in transient cell density distributions and swimming performance. We found that incubation of isogeneic E. coli with different inoculum sizes eventually generated different swimming phenotype distributions. Interestingly, incubation with antimicrobials promoted bacterial chemotaxis in specific cases, despite growth inhibition. Moreover, E. coli filamentation in the presence of antibiotics was assessed, and the impact on motility was evaluated. We propose that the observation of traveling bands can be explored as an alternative for fast antimicrobial susceptibility testing. |
format | Online Article Text |
id | pubmed-8536744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-85367442021-11-04 Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive Liu, Yang Lehnert, Thomas Gijs, Martin A. M. Microsyst Nanoeng Article Phenotypic diversity in bacterial flagella-induced motility leads to complex collective swimming patterns, appearing as traveling bands with transient locally enhanced cell densities. Traveling bands are known to be a bacterial chemotactic response to self-generated nutrient gradients during growth in resource-limited microenvironments. In this work, we studied different parameters of Escherichia coli (E. coli) collective migration, in particular the quantity of bacteria introduced initially in a microfluidic chip (inoculum size) and their exposure to antibiotics (ampicillin, ciprofloxacin, and gentamicin). We developed a hybrid polymer-glass chip with an intermediate optical adhesive layer featuring the microfluidic channel, enabling high-content imaging of the migration dynamics in a single bacterial layer, i.e., bacteria are confined in a quasi-2D space that is fully observable with a high-magnification microscope objective. On-chip bacterial motility and traveling band analysis was performed based on individual bacterial trajectories by means of custom-developed algorithms. Quantifications of swimming speed, tumble bias and effective diffusion properties allowed the assessment of phenotypic heterogeneity, resulting in variations in transient cell density distributions and swimming performance. We found that incubation of isogeneic E. coli with different inoculum sizes eventually generated different swimming phenotype distributions. Interestingly, incubation with antimicrobials promoted bacterial chemotaxis in specific cases, despite growth inhibition. Moreover, E. coli filamentation in the presence of antibiotics was assessed, and the impact on motility was evaluated. We propose that the observation of traveling bands can be explored as an alternative for fast antimicrobial susceptibility testing. Nature Publishing Group UK 2021-10-22 /pmc/articles/PMC8536744/ /pubmed/34745645 http://dx.doi.org/10.1038/s41378-021-00309-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Liu, Yang Lehnert, Thomas Gijs, Martin A. M. Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive |
title | Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive |
title_full | Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive |
title_fullStr | Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive |
title_full_unstemmed | Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive |
title_short | Effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive |
title_sort | effect of inoculum size and antibiotics on bacterial traveling bands in a thin microchannel defined by optical adhesive |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536744/ https://www.ncbi.nlm.nih.gov/pubmed/34745645 http://dx.doi.org/10.1038/s41378-021-00309-3 |
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