Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages

NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon Salmon...

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Autores principales: Dong, Xingchen, Chen, Lin-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536780/
https://www.ncbi.nlm.nih.gov/pubmed/34723211
http://dx.doi.org/10.1016/j.xpro.2021.100894
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author Dong, Xingchen
Chen, Lin-Feng
author_facet Dong, Xingchen
Chen, Lin-Feng
author_sort Dong, Xingchen
collection PubMed
description NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon Salmonella Typhimurium infection. The techniques can be adapted to monitoring the activation of other types of inflammasomes and pathogenic stimuli. For comprehensive details on the use and execution of this protocol, please refer to Dong et al. (2021).
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spelling pubmed-85367802021-10-29 Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages Dong, Xingchen Chen, Lin-Feng STAR Protoc Protocol NLR family CARD domain containing protein 4 (NLRC4) inflammasome activation and the associated pyroptosis are critical for protection against infection by bacterial pathogens. This protocol presents a detailed procedure to activate and measure NLRC4 inflammasome activation and pyroptosis upon Salmonella Typhimurium infection. The techniques can be adapted to monitoring the activation of other types of inflammasomes and pathogenic stimuli. For comprehensive details on the use and execution of this protocol, please refer to Dong et al. (2021). Elsevier 2021-10-19 /pmc/articles/PMC8536780/ /pubmed/34723211 http://dx.doi.org/10.1016/j.xpro.2021.100894 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Dong, Xingchen
Chen, Lin-Feng
Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
title Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
title_full Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
title_fullStr Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
title_full_unstemmed Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
title_short Protocol for measuring NLRC4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
title_sort protocol for measuring nlrc4 inflammasome activation and pyroptosis in murine bone-marrow-derived macrophages
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536780/
https://www.ncbi.nlm.nih.gov/pubmed/34723211
http://dx.doi.org/10.1016/j.xpro.2021.100894
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