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Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP

Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament retrograde...

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Detalles Bibliográficos
Autores principales: Kastian, Ria Fajarwati, Minegishi, Takunori, Inagaki, Naoyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536781/
https://www.ncbi.nlm.nih.gov/pubmed/34723214
http://dx.doi.org/10.1016/j.xpro.2021.100904
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author Kastian, Ria Fajarwati
Minegishi, Takunori
Inagaki, Naoyuki
author_facet Kastian, Ria Fajarwati
Minegishi, Takunori
Inagaki, Naoyuki
author_sort Kastian, Ria Fajarwati
collection PubMed
description Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament retrograde flow and actin polymerization within spines using a standard epi-fluorescence microscope. In combination with chemical long-term potentiation, this protocol allows us to quantify clutch coupling efficiency and actin polymerization rate, which are essential variables for generating forces for activity-dependent spine enlargement. For complete details on the use and execution of this protocol, please refer to Kastian et al. (2021).
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spelling pubmed-85367812021-10-29 Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP Kastian, Ria Fajarwati Minegishi, Takunori Inagaki, Naoyuki STAR Protoc Protocol Dendritic spine enlargement by synaptic activation is thought to increase synaptic efficacy underlying learning and memory. This process requires forces generated by actin polymerization and actin-adhesion coupling (clutch coupling). Here, we describe a protocol to monitor actin filament retrograde flow and actin polymerization within spines using a standard epi-fluorescence microscope. In combination with chemical long-term potentiation, this protocol allows us to quantify clutch coupling efficiency and actin polymerization rate, which are essential variables for generating forces for activity-dependent spine enlargement. For complete details on the use and execution of this protocol, please refer to Kastian et al. (2021). Elsevier 2021-10-19 /pmc/articles/PMC8536781/ /pubmed/34723214 http://dx.doi.org/10.1016/j.xpro.2021.100904 Text en © 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kastian, Ria Fajarwati
Minegishi, Takunori
Inagaki, Naoyuki
Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
title Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
title_full Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
title_fullStr Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
title_full_unstemmed Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
title_short Simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical LTP
title_sort simultaneous analyses of clutch coupling and actin polymerization in dendritic spines of rodent hippocampal neurons during chemical ltp
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536781/
https://www.ncbi.nlm.nih.gov/pubmed/34723214
http://dx.doi.org/10.1016/j.xpro.2021.100904
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