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Isolation of high-quality total RNA and RNA sequencing of single bovine oocytes

Studying individual mammalian oocytes has been extremely valuable for the understanding of the molecular composition of oocytes including RNA storage. Here, a detailed protocol for isolation of oocytes, extraction of total RNA from single oocytes followed by full-length cDNA amplification, and libra...

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Detalles Bibliográficos
Autor principal:	Biase, Fernando H.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8536786/ https://www.ncbi.nlm.nih.gov/pubmed/34723212 http://dx.doi.org/10.1016/j.xpro.2021.100895

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