Synaptic Interactions in Scorpion Peg Sensilla Appear to Maintain Chemosensory Neurons within Dynamic Firing Range

SIMPLE SUMMARY: Scorpions have unusual taste organs called pectines that they drag over the ground as they walk. Minute, peg-shaped sensilla adorn the ground-facing surfaces of the pectines, and each of these “pegs” contains several chemosensitive neurons and at least one mechanosensitive neuron. Of particular interest is that some of these neurons interact synaptically at the level of the peg sensillum prior to relay to the scorpion brain. Here we use a technique called “conditional cross-interval correlation analysis” to show that heightened activity of two of the neurons appears to induce a third neuron, which in turn inhibits the previous two. We suggest that the dynamics of this simple feedback circuit might serve to maintain the sensory neurons in a sensitive range so that substrate information can be accurately detected and processed, such as during tracking sexual pheromone trails and/or recapitulating home-directed training paths. ABSTRACT: Scorpions have elaborate chemo-tactile organs called pectines on their ventral mesosoma. The teeth of the comb-like pectines support thousands of minute projections called peg sensilla (a.k.a. “pegs”), each containing approximately 10 chemosensory neurons. Males use pectines to detect pheromones released by females, and both sexes apparently use pectines to find prey and navigate to home retreats. Electrophysiological recordings from pegs of Paruroctonus utahensis reveal three spontaneously active cells (A(1), A(2), and B), which appear to interact synaptically. We made long-term extracellular recordings from the bases of peg sensilla and used a combination of conditional cross-interval and conditional interspike-interval analyses to assess the temporal dynamics of the A and B spike trains. Like previous studies, we found that A cells are inhibited by B cells for tens of milliseconds. However, after normalizing our records, we also found clear evidence that the A cells excite the B cells. This simple local circuit appears to maintain the A cells in a dynamic firing range and may have important implications for tracking pheromonal trails and sensing substrate chemistry for navigation.