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A Portable Device for Simple Exosome Separation from Biological Samples

Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological...

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Autores principales: Chen, Wenwen, Xie, Yingying, Chang, Yuang, Xu, Yuhai, Zhao, Mengqian, Deng, Pengwei, Qin, Jianhua, Li, Hongjing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537198/
https://www.ncbi.nlm.nih.gov/pubmed/34683233
http://dx.doi.org/10.3390/mi12101182
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author Chen, Wenwen
Xie, Yingying
Chang, Yuang
Xu, Yuhai
Zhao, Mengqian
Deng, Pengwei
Qin, Jianhua
Li, Hongjing
author_facet Chen, Wenwen
Xie, Yingying
Chang, Yuang
Xu, Yuhai
Zhao, Mengqian
Deng, Pengwei
Qin, Jianhua
Li, Hongjing
author_sort Chen, Wenwen
collection PubMed
description Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis.
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spelling pubmed-85371982021-10-24 A Portable Device for Simple Exosome Separation from Biological Samples Chen, Wenwen Xie, Yingying Chang, Yuang Xu, Yuhai Zhao, Mengqian Deng, Pengwei Qin, Jianhua Li, Hongjing Micromachines (Basel) Article Exosomes are membrane-bound nanovesicles secreted by most types of cells, which contain a series of biologically important molecules, such as miRNAs, proteins, and lipids, etc. Emerging evidence show that exosomes can affect the physiological status of cells and are involved in various pathological processes. However, due to their small size and density close to body fluids, it is challenging to separate exosomes from a small volume of biological samples in a simple manner. Herein, we propose a new strategy for isolating circulating exosomes from biological samples in a portable device. This method synergistically integrates chitosan electrostatic-adsorption, scaffold substrates, and shuttle flow to enable the highly effective capture of circulating exosomes with a recovery rate of over 80% within 20 min, which is much better than the performance of traditional ultracentrifugation (5–25%, 3 h). Besides, the isolated exosomes from samples could be lysed in situ and further subjected to RNA concentration detection and protein analysis. In particular, all the necessary procedures for exosome separation could be integrated into a single device without the need for bulky equipment. This established device is portable and easy to operate, which provides a promising platform for the study of exosome biology and clinical diagnosis. MDPI 2021-09-29 /pmc/articles/PMC8537198/ /pubmed/34683233 http://dx.doi.org/10.3390/mi12101182 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Wenwen
Xie, Yingying
Chang, Yuang
Xu, Yuhai
Zhao, Mengqian
Deng, Pengwei
Qin, Jianhua
Li, Hongjing
A Portable Device for Simple Exosome Separation from Biological Samples
title A Portable Device for Simple Exosome Separation from Biological Samples
title_full A Portable Device for Simple Exosome Separation from Biological Samples
title_fullStr A Portable Device for Simple Exosome Separation from Biological Samples
title_full_unstemmed A Portable Device for Simple Exosome Separation from Biological Samples
title_short A Portable Device for Simple Exosome Separation from Biological Samples
title_sort portable device for simple exosome separation from biological samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537198/
https://www.ncbi.nlm.nih.gov/pubmed/34683233
http://dx.doi.org/10.3390/mi12101182
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