Cargando…

Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts

This study explored and investigated how zearalenone (ZEA) affects the morphology of small intestine and the distribution and expression of ghrelin and proliferating cell nuclear antigen (PCNA) in the small intestine of weaned gilts. A total of 20 weaned gilts (42-day-old, D × L × Y, weighing 12.84...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Quanwei, Huang, Libo, Leng, Bo, Li, Yang, Jiao, Ning, Jiang, Shuzhen, Yang, Weiren, Yuan, Xuejun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537219/
https://www.ncbi.nlm.nih.gov/pubmed/34679029
http://dx.doi.org/10.3390/toxins13100736
_version_ 1784588197620088832
author Zhang, Quanwei
Huang, Libo
Leng, Bo
Li, Yang
Jiao, Ning
Jiang, Shuzhen
Yang, Weiren
Yuan, Xuejun
author_facet Zhang, Quanwei
Huang, Libo
Leng, Bo
Li, Yang
Jiao, Ning
Jiang, Shuzhen
Yang, Weiren
Yuan, Xuejun
author_sort Zhang, Quanwei
collection PubMed
description This study explored and investigated how zearalenone (ZEA) affects the morphology of small intestine and the distribution and expression of ghrelin and proliferating cell nuclear antigen (PCNA) in the small intestine of weaned gilts. A total of 20 weaned gilts (42-day-old, D × L × Y, weighing 12.84 ± 0.26 kg) were divided into the control and ZEA groups (ZEA at 1.04 mg/kg in diet) in a 35-d study. Histological observations of the small intestines revealed that villus injuries of the duodenum, jejunum and ileum, such as atrophy, retardation and branching dysfunction, were observed in the ZEA treatment. The villi branch of the ileum in the ZEA group was obviously decreased compared to that of the ileum, jejunum and duodenum, and the number of lymphoid nodules of the ileum was increased. Additionally, the effect of ZEA (1.04 mg/kg) was decreased by the immunoreactivity and distribution of ghrelin and PCNA in the duodenal and jejunal mucosal epithelial cells. Interestingly, ZEA increased the immunoreactivity of ghrelin in the ileal mucosal epithelial cells and decreased the immunoreactivity expression of PCNA in the gland epithelium of the small intestine. In conclusion, ZEA (1.04 mg/kg) had adverse effects on the development and the absorptive capacity of the villi of the intestines; yet, the small intestine could resist or ameliorate the adverse effects of ZEA by changing the autocrine of ghrelin in intestinal epithelial cells.
format Online
Article
Text
id pubmed-8537219
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85372192021-10-24 Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts Zhang, Quanwei Huang, Libo Leng, Bo Li, Yang Jiao, Ning Jiang, Shuzhen Yang, Weiren Yuan, Xuejun Toxins (Basel) Article This study explored and investigated how zearalenone (ZEA) affects the morphology of small intestine and the distribution and expression of ghrelin and proliferating cell nuclear antigen (PCNA) in the small intestine of weaned gilts. A total of 20 weaned gilts (42-day-old, D × L × Y, weighing 12.84 ± 0.26 kg) were divided into the control and ZEA groups (ZEA at 1.04 mg/kg in diet) in a 35-d study. Histological observations of the small intestines revealed that villus injuries of the duodenum, jejunum and ileum, such as atrophy, retardation and branching dysfunction, were observed in the ZEA treatment. The villi branch of the ileum in the ZEA group was obviously decreased compared to that of the ileum, jejunum and duodenum, and the number of lymphoid nodules of the ileum was increased. Additionally, the effect of ZEA (1.04 mg/kg) was decreased by the immunoreactivity and distribution of ghrelin and PCNA in the duodenal and jejunal mucosal epithelial cells. Interestingly, ZEA increased the immunoreactivity of ghrelin in the ileal mucosal epithelial cells and decreased the immunoreactivity expression of PCNA in the gland epithelium of the small intestine. In conclusion, ZEA (1.04 mg/kg) had adverse effects on the development and the absorptive capacity of the villi of the intestines; yet, the small intestine could resist or ameliorate the adverse effects of ZEA by changing the autocrine of ghrelin in intestinal epithelial cells. MDPI 2021-10-19 /pmc/articles/PMC8537219/ /pubmed/34679029 http://dx.doi.org/10.3390/toxins13100736 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Quanwei
Huang, Libo
Leng, Bo
Li, Yang
Jiao, Ning
Jiang, Shuzhen
Yang, Weiren
Yuan, Xuejun
Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts
title Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts
title_full Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts
title_fullStr Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts
title_full_unstemmed Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts
title_short Zearalenone Affect the Intestinal Villi Associated with the Distribution and the Expression of Ghrelin and Proliferating Cell Nuclear Antigen in Weaned Gilts
title_sort zearalenone affect the intestinal villi associated with the distribution and the expression of ghrelin and proliferating cell nuclear antigen in weaned gilts
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537219/
https://www.ncbi.nlm.nih.gov/pubmed/34679029
http://dx.doi.org/10.3390/toxins13100736
work_keys_str_mv AT zhangquanwei zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts
AT huanglibo zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts
AT lengbo zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts
AT liyang zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts
AT jiaoning zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts
AT jiangshuzhen zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts
AT yangweiren zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts
AT yuanxuejun zearalenoneaffecttheintestinalvilliassociatedwiththedistributionandtheexpressionofghrelinandproliferatingcellnuclearantigeninweanedgilts