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Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms

Bacterial biofilm constitutes a strong barrier against the penetration of drugs and against the action of the host immune system causing persistent infections hardly treatable by antibiotic therapy. Helicobacter pylori (Hp), the main causative agent for gastritis, peptic ulcer and gastric adenocarci...

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Autores principales: Battisti, Antonella, Morici, Paola, Sgarbossa, Antonella
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537233/
https://www.ncbi.nlm.nih.gov/pubmed/34683966
http://dx.doi.org/10.3390/pharmaceutics13101674
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author Battisti, Antonella
Morici, Paola
Sgarbossa, Antonella
author_facet Battisti, Antonella
Morici, Paola
Sgarbossa, Antonella
author_sort Battisti, Antonella
collection PubMed
description Bacterial biofilm constitutes a strong barrier against the penetration of drugs and against the action of the host immune system causing persistent infections hardly treatable by antibiotic therapy. Helicobacter pylori (Hp), the main causative agent for gastritis, peptic ulcer and gastric adenocarcinoma, can form a biofilm composed by an exopolysaccharide matrix layer covering the gastric surface where the bacterial cells become resistant and tolerant to the commonly used antibiotics clarithromycin, amoxicillin and metronidazole. Antimicrobial PhotoDynamic Therapy (aPDT) was proposed as an alternative treatment strategy for eradicating bacterial infections, particularly effective for Hp since this microorganism produces and stores up photosensitizing porphyrins. The knowledge of the photophysical characteristics of Hp porphyrins in their physiological biofilm microenvironment is crucial to implement and optimize the photodynamic treatment. Fluorescence lifetime imaging microscopy (FLIM) of intrinsic bacterial porphyrins was performed and data were analyzed by the ‘fit-free’ phasor approach in order to map the distribution of the different fluorescent species within Hp biofilm. Porphyrins inside bacteria were easily distinguished from those dispersed in the matrix suggesting FLIM-phasor technique as a sensitive and rapid tool to monitor the photosensitizer distribution inside bacterial biofilms and to better orientate the phototherapeutic strategy.
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spelling pubmed-85372332021-10-24 Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms Battisti, Antonella Morici, Paola Sgarbossa, Antonella Pharmaceutics Communication Bacterial biofilm constitutes a strong barrier against the penetration of drugs and against the action of the host immune system causing persistent infections hardly treatable by antibiotic therapy. Helicobacter pylori (Hp), the main causative agent for gastritis, peptic ulcer and gastric adenocarcinoma, can form a biofilm composed by an exopolysaccharide matrix layer covering the gastric surface where the bacterial cells become resistant and tolerant to the commonly used antibiotics clarithromycin, amoxicillin and metronidazole. Antimicrobial PhotoDynamic Therapy (aPDT) was proposed as an alternative treatment strategy for eradicating bacterial infections, particularly effective for Hp since this microorganism produces and stores up photosensitizing porphyrins. The knowledge of the photophysical characteristics of Hp porphyrins in their physiological biofilm microenvironment is crucial to implement and optimize the photodynamic treatment. Fluorescence lifetime imaging microscopy (FLIM) of intrinsic bacterial porphyrins was performed and data were analyzed by the ‘fit-free’ phasor approach in order to map the distribution of the different fluorescent species within Hp biofilm. Porphyrins inside bacteria were easily distinguished from those dispersed in the matrix suggesting FLIM-phasor technique as a sensitive and rapid tool to monitor the photosensitizer distribution inside bacterial biofilms and to better orientate the phototherapeutic strategy. MDPI 2021-10-13 /pmc/articles/PMC8537233/ /pubmed/34683966 http://dx.doi.org/10.3390/pharmaceutics13101674 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Battisti, Antonella
Morici, Paola
Sgarbossa, Antonella
Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
title Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
title_full Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
title_fullStr Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
title_full_unstemmed Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
title_short Fluorescence Lifetime Imaging Microscopy of Porphyrins in Helicobacter pylori Biofilms
title_sort fluorescence lifetime imaging microscopy of porphyrins in helicobacter pylori biofilms
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537233/
https://www.ncbi.nlm.nih.gov/pubmed/34683966
http://dx.doi.org/10.3390/pharmaceutics13101674
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