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Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity

Limosilactobacillus fermentum (ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against Candida species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potentia...

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Autores principales: dos Santos, Camilla I., Campos, Carmem D. L., Nunes-Neto, Wallace R., do Carmo, Monique S., Nogueira, Flávio A. B., Ferreira, Rômulo M., Costa, Ennio P. S., Gonzaga, Laoane F., Araújo, Jéssica M. M., Monteiro, Joveliane M., Monteiro, Cinara Regina A. V., Platner, Fernanda S., Figueiredo, Isabella F. S., Holanda, Rodrigo A., Monteiro, Silvio G., Fernandes, Elizabeth S., Monteiro, Andrea S., Monteiro-Neto, Valério
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537286/
https://www.ncbi.nlm.nih.gov/pubmed/34682216
http://dx.doi.org/10.3390/jof7100794
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author dos Santos, Camilla I.
Campos, Carmem D. L.
Nunes-Neto, Wallace R.
do Carmo, Monique S.
Nogueira, Flávio A. B.
Ferreira, Rômulo M.
Costa, Ennio P. S.
Gonzaga, Laoane F.
Araújo, Jéssica M. M.
Monteiro, Joveliane M.
Monteiro, Cinara Regina A. V.
Platner, Fernanda S.
Figueiredo, Isabella F. S.
Holanda, Rodrigo A.
Monteiro, Silvio G.
Fernandes, Elizabeth S.
Monteiro, Andrea S.
Monteiro-Neto, Valério
author_facet dos Santos, Camilla I.
Campos, Carmem D. L.
Nunes-Neto, Wallace R.
do Carmo, Monique S.
Nogueira, Flávio A. B.
Ferreira, Rômulo M.
Costa, Ennio P. S.
Gonzaga, Laoane F.
Araújo, Jéssica M. M.
Monteiro, Joveliane M.
Monteiro, Cinara Regina A. V.
Platner, Fernanda S.
Figueiredo, Isabella F. S.
Holanda, Rodrigo A.
Monteiro, Silvio G.
Fernandes, Elizabeth S.
Monteiro, Andrea S.
Monteiro-Neto, Valério
author_sort dos Santos, Camilla I.
collection PubMed
description Limosilactobacillus fermentum (ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against Candida species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potential. The ATCC 23271 genome was found to contain 2,193,335 bp, with 2123 protein-coding sequences. Phylogenetic analysis revealed that the ATCC 23271 strain shares 941 gene clusters with six other probiotic strains of L. fermentum. Putative genes known to confer probiotic properties have been identified in the genome, including genes related to adhesion, tolerance to acidic pH and bile salts, tolerance to oxidative stress, and metabolism and transport of sugars and other compounds. A search for bacteriocin genes revealed a sequence 48% similar to that of enterolysin A, a protein from Enterococcus faecalis. However, in vitro assays confirmed that the strain has inhibitory activity on the growth of Candida species and also interferes with their adhesion to HeLa cells. In silico analyses demonstrated a high probability of the protein with antimicrobial activity. Our data reveal the genome features of L. fermentum ATCC 23271, which may provide insight into its future use given the functional benefits, especially against Candida infections.
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spelling pubmed-85372862021-10-24 Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity dos Santos, Camilla I. Campos, Carmem D. L. Nunes-Neto, Wallace R. do Carmo, Monique S. Nogueira, Flávio A. B. Ferreira, Rômulo M. Costa, Ennio P. S. Gonzaga, Laoane F. Araújo, Jéssica M. M. Monteiro, Joveliane M. Monteiro, Cinara Regina A. V. Platner, Fernanda S. Figueiredo, Isabella F. S. Holanda, Rodrigo A. Monteiro, Silvio G. Fernandes, Elizabeth S. Monteiro, Andrea S. Monteiro-Neto, Valério J Fungi (Basel) Article Limosilactobacillus fermentum (ATCC 23271) was originally isolated from the human intestine and has displayed antimicrobial activity, primarily against Candida species. Complete genome sequencing and comparative analyses were performed to elucidate the genetic basis underlying its probiotic potential. The ATCC 23271 genome was found to contain 2,193,335 bp, with 2123 protein-coding sequences. Phylogenetic analysis revealed that the ATCC 23271 strain shares 941 gene clusters with six other probiotic strains of L. fermentum. Putative genes known to confer probiotic properties have been identified in the genome, including genes related to adhesion, tolerance to acidic pH and bile salts, tolerance to oxidative stress, and metabolism and transport of sugars and other compounds. A search for bacteriocin genes revealed a sequence 48% similar to that of enterolysin A, a protein from Enterococcus faecalis. However, in vitro assays confirmed that the strain has inhibitory activity on the growth of Candida species and also interferes with their adhesion to HeLa cells. In silico analyses demonstrated a high probability of the protein with antimicrobial activity. Our data reveal the genome features of L. fermentum ATCC 23271, which may provide insight into its future use given the functional benefits, especially against Candida infections. MDPI 2021-09-24 /pmc/articles/PMC8537286/ /pubmed/34682216 http://dx.doi.org/10.3390/jof7100794 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
dos Santos, Camilla I.
Campos, Carmem D. L.
Nunes-Neto, Wallace R.
do Carmo, Monique S.
Nogueira, Flávio A. B.
Ferreira, Rômulo M.
Costa, Ennio P. S.
Gonzaga, Laoane F.
Araújo, Jéssica M. M.
Monteiro, Joveliane M.
Monteiro, Cinara Regina A. V.
Platner, Fernanda S.
Figueiredo, Isabella F. S.
Holanda, Rodrigo A.
Monteiro, Silvio G.
Fernandes, Elizabeth S.
Monteiro, Andrea S.
Monteiro-Neto, Valério
Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity
title Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity
title_full Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity
title_fullStr Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity
title_full_unstemmed Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity
title_short Genomic Analysis of Limosilactobacillus fermentum ATCC 23271, a Potential Probiotic Strain with Anti-Candida Activity
title_sort genomic analysis of limosilactobacillus fermentum atcc 23271, a potential probiotic strain with anti-candida activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537286/
https://www.ncbi.nlm.nih.gov/pubmed/34682216
http://dx.doi.org/10.3390/jof7100794
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