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Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination

Myelin sheaths are essential in maintaining the integrity of axons. Development of the platform for in vitro myelination would be especially useful for demyelinating disease modeling and drug screening. In this study, a fiber scaffold with a core–shell structure was prepared in one step by the coaxi...

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Autores principales: Liu, Zhepeng, Wang, Jing, Chen, Haini, Zhang, Guanyu, Lv, Zhuman, Li, Yijun, Zhao, Shoujin, Li, Wenlin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537353/
https://www.ncbi.nlm.nih.gov/pubmed/34685353
http://dx.doi.org/10.3390/polym13203595
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author Liu, Zhepeng
Wang, Jing
Chen, Haini
Zhang, Guanyu
Lv, Zhuman
Li, Yijun
Zhao, Shoujin
Li, Wenlin
author_facet Liu, Zhepeng
Wang, Jing
Chen, Haini
Zhang, Guanyu
Lv, Zhuman
Li, Yijun
Zhao, Shoujin
Li, Wenlin
author_sort Liu, Zhepeng
collection PubMed
description Myelin sheaths are essential in maintaining the integrity of axons. Development of the platform for in vitro myelination would be especially useful for demyelinating disease modeling and drug screening. In this study, a fiber scaffold with a core–shell structure was prepared in one step by the coaxial electrospinning method. A high-molecular-weight polymer poly-L-lactic acid (PLLA) was used as the core, while the shell was a natural polymer material such as hyaluronic acid (HA), sodium alginate (SA), or chitosan (CS). The morphology, differential scanning calorimetry (DSC), Fourier transform infrared spectra (FTIR), contact angle, viability assay, and in vitro myelination by oligodendrocytes were characterized. The results showed that such fibers are bead-free and continuous, with an average size from 294 ± 53 to 390 ± 54 nm. The DSC and FTIR curves indicated no changes in the phase state of coaxial brackets. Hyaluronic acid/PLLA coaxial fibers had the minimum contact angle (53.1° ± 0.24°). Myelin sheaths were wrapped around a coaxial electrospun scaffold modified with water-soluble materials after a 14-day incubation. All results suggest that such a scaffold prepared by coaxial electrospinning potentially provides a novel platform for oligodendrocyte myelination.
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spelling pubmed-85373532021-10-24 Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination Liu, Zhepeng Wang, Jing Chen, Haini Zhang, Guanyu Lv, Zhuman Li, Yijun Zhao, Shoujin Li, Wenlin Polymers (Basel) Article Myelin sheaths are essential in maintaining the integrity of axons. Development of the platform for in vitro myelination would be especially useful for demyelinating disease modeling and drug screening. In this study, a fiber scaffold with a core–shell structure was prepared in one step by the coaxial electrospinning method. A high-molecular-weight polymer poly-L-lactic acid (PLLA) was used as the core, while the shell was a natural polymer material such as hyaluronic acid (HA), sodium alginate (SA), or chitosan (CS). The morphology, differential scanning calorimetry (DSC), Fourier transform infrared spectra (FTIR), contact angle, viability assay, and in vitro myelination by oligodendrocytes were characterized. The results showed that such fibers are bead-free and continuous, with an average size from 294 ± 53 to 390 ± 54 nm. The DSC and FTIR curves indicated no changes in the phase state of coaxial brackets. Hyaluronic acid/PLLA coaxial fibers had the minimum contact angle (53.1° ± 0.24°). Myelin sheaths were wrapped around a coaxial electrospun scaffold modified with water-soluble materials after a 14-day incubation. All results suggest that such a scaffold prepared by coaxial electrospinning potentially provides a novel platform for oligodendrocyte myelination. MDPI 2021-10-19 /pmc/articles/PMC8537353/ /pubmed/34685353 http://dx.doi.org/10.3390/polym13203595 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhepeng
Wang, Jing
Chen, Haini
Zhang, Guanyu
Lv, Zhuman
Li, Yijun
Zhao, Shoujin
Li, Wenlin
Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination
title Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination
title_full Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination
title_fullStr Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination
title_full_unstemmed Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination
title_short Coaxial Electrospun PLLA Fibers Modified with Water-Soluble Materials for Oligodendrocyte Myelination
title_sort coaxial electrospun plla fibers modified with water-soluble materials for oligodendrocyte myelination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537353/
https://www.ncbi.nlm.nih.gov/pubmed/34685353
http://dx.doi.org/10.3390/polym13203595
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