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Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses

Antibodies targeting the spike (S) and nucleocapsid (N) proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential tools. In addition to important roles in the treatment and diagnosis of infection, the availability of high-quality specific antibodies for the S and N prote...

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Autores principales: Kumar, Rinki, Christensen, Neil D., Kaddis Maldonado, Rebecca J., Bewley, Maria C., Ostman, Alexandria, Sudol, Malgorzata, Chen, Eunice C., Buchkovich, Natalie W., Gontu, Abhinay, Surendran Nair, Meera, Nissly, Ruth H., Minns, Allen M., Kapur, Vivek, Rossi, Randall, Kuchipudi, Suresh V., Lindner, Scott E., Parent, Leslie J., Flanagan, John M., Buchkovich, Nicholas J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537396/
https://www.ncbi.nlm.nih.gov/pubmed/34696329
http://dx.doi.org/10.3390/v13101899
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author Kumar, Rinki
Christensen, Neil D.
Kaddis Maldonado, Rebecca J.
Bewley, Maria C.
Ostman, Alexandria
Sudol, Malgorzata
Chen, Eunice C.
Buchkovich, Natalie W.
Gontu, Abhinay
Surendran Nair, Meera
Nissly, Ruth H.
Minns, Allen M.
Kapur, Vivek
Rossi, Randall
Kuchipudi, Suresh V.
Lindner, Scott E.
Parent, Leslie J.
Flanagan, John M.
Buchkovich, Nicholas J.
author_facet Kumar, Rinki
Christensen, Neil D.
Kaddis Maldonado, Rebecca J.
Bewley, Maria C.
Ostman, Alexandria
Sudol, Malgorzata
Chen, Eunice C.
Buchkovich, Natalie W.
Gontu, Abhinay
Surendran Nair, Meera
Nissly, Ruth H.
Minns, Allen M.
Kapur, Vivek
Rossi, Randall
Kuchipudi, Suresh V.
Lindner, Scott E.
Parent, Leslie J.
Flanagan, John M.
Buchkovich, Nicholas J.
author_sort Kumar, Rinki
collection PubMed
description Antibodies targeting the spike (S) and nucleocapsid (N) proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential tools. In addition to important roles in the treatment and diagnosis of infection, the availability of high-quality specific antibodies for the S and N proteins is essential to facilitate basic research of virus replication and in the characterization of mutations responsible for variants of concern. We have developed panels of mouse and rabbit monoclonal antibodies (mAbs) to the SARS-CoV-2 spike receptor-binding domain (S-RBD) and N protein for functional and antigenic analyses. The mAbs to the S-RBD were tested for neutralization of native SARS-CoV-2, with several exhibiting neutralizing activity. The panels of mAbs to the N protein were assessed for cross-reactivity with the SARS-CoV and Middle East respiratory syndrome (MERS)-CoV N proteins and could be subdivided into sets that showed unique specificity for SARS-CoV-2 N protein, cross-reactivity between SARS-CoV-2 and SARS-CoV N proteins only, or cross-reactivity to all three coronavirus N proteins tested. Partial mapping of N-reactive mAbs were conducted using truncated fragments of the SARS-CoV-2 N protein and revealed near complete coverage of the N protein. Collectively, these sets of mouse and rabbit monoclonal antibodies can be used to examine structure/function studies for N proteins and to define the surface location of virus neutralizing epitopes on the RBD of the S protein.
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spelling pubmed-85373962021-10-24 Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses Kumar, Rinki Christensen, Neil D. Kaddis Maldonado, Rebecca J. Bewley, Maria C. Ostman, Alexandria Sudol, Malgorzata Chen, Eunice C. Buchkovich, Natalie W. Gontu, Abhinay Surendran Nair, Meera Nissly, Ruth H. Minns, Allen M. Kapur, Vivek Rossi, Randall Kuchipudi, Suresh V. Lindner, Scott E. Parent, Leslie J. Flanagan, John M. Buchkovich, Nicholas J. Viruses Article Antibodies targeting the spike (S) and nucleocapsid (N) proteins of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are essential tools. In addition to important roles in the treatment and diagnosis of infection, the availability of high-quality specific antibodies for the S and N proteins is essential to facilitate basic research of virus replication and in the characterization of mutations responsible for variants of concern. We have developed panels of mouse and rabbit monoclonal antibodies (mAbs) to the SARS-CoV-2 spike receptor-binding domain (S-RBD) and N protein for functional and antigenic analyses. The mAbs to the S-RBD were tested for neutralization of native SARS-CoV-2, with several exhibiting neutralizing activity. The panels of mAbs to the N protein were assessed for cross-reactivity with the SARS-CoV and Middle East respiratory syndrome (MERS)-CoV N proteins and could be subdivided into sets that showed unique specificity for SARS-CoV-2 N protein, cross-reactivity between SARS-CoV-2 and SARS-CoV N proteins only, or cross-reactivity to all three coronavirus N proteins tested. Partial mapping of N-reactive mAbs were conducted using truncated fragments of the SARS-CoV-2 N protein and revealed near complete coverage of the N protein. Collectively, these sets of mouse and rabbit monoclonal antibodies can be used to examine structure/function studies for N proteins and to define the surface location of virus neutralizing epitopes on the RBD of the S protein. MDPI 2021-09-22 /pmc/articles/PMC8537396/ /pubmed/34696329 http://dx.doi.org/10.3390/v13101899 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kumar, Rinki
Christensen, Neil D.
Kaddis Maldonado, Rebecca J.
Bewley, Maria C.
Ostman, Alexandria
Sudol, Malgorzata
Chen, Eunice C.
Buchkovich, Natalie W.
Gontu, Abhinay
Surendran Nair, Meera
Nissly, Ruth H.
Minns, Allen M.
Kapur, Vivek
Rossi, Randall
Kuchipudi, Suresh V.
Lindner, Scott E.
Parent, Leslie J.
Flanagan, John M.
Buchkovich, Nicholas J.
Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses
title Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses
title_full Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses
title_fullStr Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses
title_full_unstemmed Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses
title_short Monoclonal Antibodies to S and N SARS-CoV-2 Proteins as Probes to Assess Structural and Antigenic Properties of Coronaviruses
title_sort monoclonal antibodies to s and n sars-cov-2 proteins as probes to assess structural and antigenic properties of coronaviruses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537396/
https://www.ncbi.nlm.nih.gov/pubmed/34696329
http://dx.doi.org/10.3390/v13101899
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