Cargando…
Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537554/ https://www.ncbi.nlm.nih.gov/pubmed/34677528 http://dx.doi.org/10.3390/membranes11100762 |
_version_ | 1784588288507510784 |
---|---|
author | Akhiyarova, Guzel R. Ivanov, Ruslan S. Ivanov, Igor I. Finkina, Ekaterina I. Melnikova, Daria N. Bogdanov, Ivan V. Nuzhnaya, Tatyana Ovchinnikova, Tatiana V. Veselov, Dmitriy S. Kudoyarova, Guzel R. |
author_facet | Akhiyarova, Guzel R. Ivanov, Ruslan S. Ivanov, Igor I. Finkina, Ekaterina I. Melnikova, Daria N. Bogdanov, Ivan V. Nuzhnaya, Tatyana Ovchinnikova, Tatiana V. Veselov, Dmitriy S. Kudoyarova, Guzel R. |
author_sort | Akhiyarova, Guzel R. |
collection | PubMed |
description | Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and their mutual participation in suberin deposition in root endodermis of salt-stressed pea plants. Using immunohistochemistry we show for the first time NaCl induced accumulation of LTPs and ABA in the cell walls of phloem paralleled by suberin deposition in the endoderm region of pea roots. Unlike LTPs which were found localized around phloem cells, ABA was also present within phloem cells. In addition, ABA treatment resulted in both LTP and ABA accumulation in phloem cells and promoted root suberization. These results suggested the importance of NaCl-induced accumulation of ABA in increasing the abundance of LTPs and of suberin. Using molecular modeling and fluorescence spectroscopy we confirmed the ability of different plant LTPs, including pea Ps-LTP1, to bind ABA. We therefore hypothesize an involvement of plant LTPs in ABA transport (unloading from phloem) as part of the salinity adaptation mechanism. |
format | Online Article Text |
id | pubmed-8537554 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85375542021-10-24 Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots Akhiyarova, Guzel R. Ivanov, Ruslan S. Ivanov, Igor I. Finkina, Ekaterina I. Melnikova, Daria N. Bogdanov, Ivan V. Nuzhnaya, Tatyana Ovchinnikova, Tatiana V. Veselov, Dmitriy S. Kudoyarova, Guzel R. Membranes (Basel) Article Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and their mutual participation in suberin deposition in root endodermis of salt-stressed pea plants. Using immunohistochemistry we show for the first time NaCl induced accumulation of LTPs and ABA in the cell walls of phloem paralleled by suberin deposition in the endoderm region of pea roots. Unlike LTPs which were found localized around phloem cells, ABA was also present within phloem cells. In addition, ABA treatment resulted in both LTP and ABA accumulation in phloem cells and promoted root suberization. These results suggested the importance of NaCl-induced accumulation of ABA in increasing the abundance of LTPs and of suberin. Using molecular modeling and fluorescence spectroscopy we confirmed the ability of different plant LTPs, including pea Ps-LTP1, to bind ABA. We therefore hypothesize an involvement of plant LTPs in ABA transport (unloading from phloem) as part of the salinity adaptation mechanism. MDPI 2021-10-01 /pmc/articles/PMC8537554/ /pubmed/34677528 http://dx.doi.org/10.3390/membranes11100762 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Akhiyarova, Guzel R. Ivanov, Ruslan S. Ivanov, Igor I. Finkina, Ekaterina I. Melnikova, Daria N. Bogdanov, Ivan V. Nuzhnaya, Tatyana Ovchinnikova, Tatiana V. Veselov, Dmitriy S. Kudoyarova, Guzel R. Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
title | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
title_full | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
title_fullStr | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
title_full_unstemmed | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
title_short | Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots |
title_sort | effects of salinity and abscisic acid on lipid transfer protein accumulation, suberin deposition and hydraulic conductance in pea roots |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537554/ https://www.ncbi.nlm.nih.gov/pubmed/34677528 http://dx.doi.org/10.3390/membranes11100762 |
work_keys_str_mv | AT akhiyarovaguzelr effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT ivanovruslans effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT ivanovigori effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT finkinaekaterinai effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT melnikovadarian effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT bogdanovivanv effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT nuzhnayatatyana effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT ovchinnikovatatianav effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT veselovdmitriys effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots AT kudoyarovaguzelr effectsofsalinityandabscisicacidonlipidtransferproteinaccumulationsuberindepositionandhydraulicconductanceinpearoots |