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Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots

Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and...

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Autores principales: Akhiyarova, Guzel R., Ivanov, Ruslan S., Ivanov, Igor I., Finkina, Ekaterina I., Melnikova, Daria N., Bogdanov, Ivan V., Nuzhnaya, Tatyana, Ovchinnikova, Tatiana V., Veselov, Dmitriy S., Kudoyarova, Guzel R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537554/
https://www.ncbi.nlm.nih.gov/pubmed/34677528
http://dx.doi.org/10.3390/membranes11100762
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author Akhiyarova, Guzel R.
Ivanov, Ruslan S.
Ivanov, Igor I.
Finkina, Ekaterina I.
Melnikova, Daria N.
Bogdanov, Ivan V.
Nuzhnaya, Tatyana
Ovchinnikova, Tatiana V.
Veselov, Dmitriy S.
Kudoyarova, Guzel R.
author_facet Akhiyarova, Guzel R.
Ivanov, Ruslan S.
Ivanov, Igor I.
Finkina, Ekaterina I.
Melnikova, Daria N.
Bogdanov, Ivan V.
Nuzhnaya, Tatyana
Ovchinnikova, Tatiana V.
Veselov, Dmitriy S.
Kudoyarova, Guzel R.
author_sort Akhiyarova, Guzel R.
collection PubMed
description Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and their mutual participation in suberin deposition in root endodermis of salt-stressed pea plants. Using immunohistochemistry we show for the first time NaCl induced accumulation of LTPs and ABA in the cell walls of phloem paralleled by suberin deposition in the endoderm region of pea roots. Unlike LTPs which were found localized around phloem cells, ABA was also present within phloem cells. In addition, ABA treatment resulted in both LTP and ABA accumulation in phloem cells and promoted root suberization. These results suggested the importance of NaCl-induced accumulation of ABA in increasing the abundance of LTPs and of suberin. Using molecular modeling and fluorescence spectroscopy we confirmed the ability of different plant LTPs, including pea Ps-LTP1, to bind ABA. We therefore hypothesize an involvement of plant LTPs in ABA transport (unloading from phloem) as part of the salinity adaptation mechanism.
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spelling pubmed-85375542021-10-24 Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots Akhiyarova, Guzel R. Ivanov, Ruslan S. Ivanov, Igor I. Finkina, Ekaterina I. Melnikova, Daria N. Bogdanov, Ivan V. Nuzhnaya, Tatyana Ovchinnikova, Tatiana V. Veselov, Dmitriy S. Kudoyarova, Guzel R. Membranes (Basel) Article Lipid transfer proteins (LTPs) participate in many important physiological processes in plants, including adaptation to stressors, e.g., salinity. Here we address the mechanism of this protective action of LTPs by studying the interaction between LTPs and abscisic acid (ABA, a “stress” hormone) and their mutual participation in suberin deposition in root endodermis of salt-stressed pea plants. Using immunohistochemistry we show for the first time NaCl induced accumulation of LTPs and ABA in the cell walls of phloem paralleled by suberin deposition in the endoderm region of pea roots. Unlike LTPs which were found localized around phloem cells, ABA was also present within phloem cells. In addition, ABA treatment resulted in both LTP and ABA accumulation in phloem cells and promoted root suberization. These results suggested the importance of NaCl-induced accumulation of ABA in increasing the abundance of LTPs and of suberin. Using molecular modeling and fluorescence spectroscopy we confirmed the ability of different plant LTPs, including pea Ps-LTP1, to bind ABA. We therefore hypothesize an involvement of plant LTPs in ABA transport (unloading from phloem) as part of the salinity adaptation mechanism. MDPI 2021-10-01 /pmc/articles/PMC8537554/ /pubmed/34677528 http://dx.doi.org/10.3390/membranes11100762 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Akhiyarova, Guzel R.
Ivanov, Ruslan S.
Ivanov, Igor I.
Finkina, Ekaterina I.
Melnikova, Daria N.
Bogdanov, Ivan V.
Nuzhnaya, Tatyana
Ovchinnikova, Tatiana V.
Veselov, Dmitriy S.
Kudoyarova, Guzel R.
Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
title Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
title_full Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
title_fullStr Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
title_full_unstemmed Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
title_short Effects of Salinity and Abscisic Acid on Lipid Transfer Protein Accumulation, Suberin Deposition and Hydraulic Conductance in Pea Roots
title_sort effects of salinity and abscisic acid on lipid transfer protein accumulation, suberin deposition and hydraulic conductance in pea roots
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537554/
https://www.ncbi.nlm.nih.gov/pubmed/34677528
http://dx.doi.org/10.3390/membranes11100762
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