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Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation

Avocado consumption is increasing year by year, and its cultivation has spread to many countries with low water availability, which threatens the sustainability and profitability of avocado orchards. However, to date, there is not much information on the behavior of commercial avocado rootstocks aga...

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Autores principales: Guillermo, Moreno-Ortega, Adela, Zumaquero, Antonio, Matas, Olivier, Nicholas A., Noëlani, van den Berg, Palomo-Ríos, Elena, Elsa, Martínez-Ferri, Clara, Pliego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537572/
https://www.ncbi.nlm.nih.gov/pubmed/34685886
http://dx.doi.org/10.3390/plants10102077
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author Guillermo, Moreno-Ortega
Adela, Zumaquero
Antonio, Matas
Olivier, Nicholas A.
Noëlani, van den Berg
Palomo-Ríos, Elena
Elsa, Martínez-Ferri
Clara, Pliego
author_facet Guillermo, Moreno-Ortega
Adela, Zumaquero
Antonio, Matas
Olivier, Nicholas A.
Noëlani, van den Berg
Palomo-Ríos, Elena
Elsa, Martínez-Ferri
Clara, Pliego
author_sort Guillermo, Moreno-Ortega
collection PubMed
description Avocado consumption is increasing year by year, and its cultivation has spread to many countries with low water availability, which threatens the sustainability and profitability of avocado orchards. However, to date, there is not much information on the behavior of commercial avocado rootstocks against drought. The aim of this research was to evaluate the physiological and molecular responses of ‘Dusa’ avocado rootstock to different levels of water stress. Plants were deficit irrigated until soil water content reached 50% (mild-WS) and 25% (severe-WS) of field capacity. Leaf water potential (Ψ(w)), net CO(2) assimilation rates (A(N)), transpiration rate (E), stomatal conductance (g(s)), and plant transpiration rates significantly decreased under both WS treatments, reaching significantly lower values in severe-WS plants. After rewatering, mild- and severe-WS plants showed a fast recovery in most physiological parameters measured. To analyze root response to different levels of drought stress, a cDNA avocado stress microarray was carried out. Plants showed a wide transcriptome response linked to the higher degree of water stress, and functional enrichment of differentially expressed genes (DEGs) revealed abundance of common sequences associated with water stress, as well as specific categories for mild-WS and severe-WS. DEGs previously linked to drought tolerance showed overexpression under both water stress levels, i.e., several transcription factors, genes related to abscisic acid (ABA) response, redox homeostasis, osmoprotection, and cell-wall organization. Taken altogether, physiological and molecular data highlight the good performance of ‘Dusa’ rootstock under low-water-availability conditions, although further water stress experiments must be carried out under field conditions.
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spelling pubmed-85375722021-10-24 Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation Guillermo, Moreno-Ortega Adela, Zumaquero Antonio, Matas Olivier, Nicholas A. Noëlani, van den Berg Palomo-Ríos, Elena Elsa, Martínez-Ferri Clara, Pliego Plants (Basel) Article Avocado consumption is increasing year by year, and its cultivation has spread to many countries with low water availability, which threatens the sustainability and profitability of avocado orchards. However, to date, there is not much information on the behavior of commercial avocado rootstocks against drought. The aim of this research was to evaluate the physiological and molecular responses of ‘Dusa’ avocado rootstock to different levels of water stress. Plants were deficit irrigated until soil water content reached 50% (mild-WS) and 25% (severe-WS) of field capacity. Leaf water potential (Ψ(w)), net CO(2) assimilation rates (A(N)), transpiration rate (E), stomatal conductance (g(s)), and plant transpiration rates significantly decreased under both WS treatments, reaching significantly lower values in severe-WS plants. After rewatering, mild- and severe-WS plants showed a fast recovery in most physiological parameters measured. To analyze root response to different levels of drought stress, a cDNA avocado stress microarray was carried out. Plants showed a wide transcriptome response linked to the higher degree of water stress, and functional enrichment of differentially expressed genes (DEGs) revealed abundance of common sequences associated with water stress, as well as specific categories for mild-WS and severe-WS. DEGs previously linked to drought tolerance showed overexpression under both water stress levels, i.e., several transcription factors, genes related to abscisic acid (ABA) response, redox homeostasis, osmoprotection, and cell-wall organization. Taken altogether, physiological and molecular data highlight the good performance of ‘Dusa’ rootstock under low-water-availability conditions, although further water stress experiments must be carried out under field conditions. MDPI 2021-09-30 /pmc/articles/PMC8537572/ /pubmed/34685886 http://dx.doi.org/10.3390/plants10102077 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guillermo, Moreno-Ortega
Adela, Zumaquero
Antonio, Matas
Olivier, Nicholas A.
Noëlani, van den Berg
Palomo-Ríos, Elena
Elsa, Martínez-Ferri
Clara, Pliego
Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation
title Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation
title_full Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation
title_fullStr Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation
title_full_unstemmed Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation
title_short Physiological and Molecular Responses of ‘Dusa’ Avocado Rootstock to Water Stress: Insights for Drought Adaptation
title_sort physiological and molecular responses of ‘dusa’ avocado rootstock to water stress: insights for drought adaptation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537572/
https://www.ncbi.nlm.nih.gov/pubmed/34685886
http://dx.doi.org/10.3390/plants10102077
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