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Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages

Advanced glycated albumin (AGE-albumin) impairs cholesterol efflux and contributes to inflammation in macrophages. The current study evaluated: (1) the persistence of the deleterious effect of AGE-albumin in cholesterol efflux and in inflammation, and (2) how metabolic control in diabetes mellitus (...

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Autores principales: Minanni, Carlos André, Machado-Lima, Adriana, Iborra, Rodrigo Tallada, Okuda, Lígia Shimabukuro, de Souza Pinto, Raphael, Santana, Monique de Fátima Mello, Lira, Aécio Lopes de Araújo, Nakandakare, Edna Regina, Côrrea-Giannella, Maria Lúcia Cardillo, Passarelli, Marisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537611/
https://www.ncbi.nlm.nih.gov/pubmed/34684632
http://dx.doi.org/10.3390/nu13103633
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author Minanni, Carlos André
Machado-Lima, Adriana
Iborra, Rodrigo Tallada
Okuda, Lígia Shimabukuro
de Souza Pinto, Raphael
Santana, Monique de Fátima Mello
Lira, Aécio Lopes de Araújo
Nakandakare, Edna Regina
Côrrea-Giannella, Maria Lúcia Cardillo
Passarelli, Marisa
author_facet Minanni, Carlos André
Machado-Lima, Adriana
Iborra, Rodrigo Tallada
Okuda, Lígia Shimabukuro
de Souza Pinto, Raphael
Santana, Monique de Fátima Mello
Lira, Aécio Lopes de Araújo
Nakandakare, Edna Regina
Côrrea-Giannella, Maria Lúcia Cardillo
Passarelli, Marisa
author_sort Minanni, Carlos André
collection PubMed
description Advanced glycated albumin (AGE-albumin) impairs cholesterol efflux and contributes to inflammation in macrophages. The current study evaluated: (1) the persistence of the deleterious effect of AGE-albumin in cholesterol efflux and in inflammation, and (2) how metabolic control in diabetes mellitus (DM) contributes to attenuate the deleterious role of AGE-albumin in macrophage cholesterol homeostasis. Methods: AGE-albumin was produced in vitro or isolated from uncontrolled DM subjects’ serum before (bGC) and after improved glycemic control (aGC). Albumin samples were incubated with bone marrow-derived macrophages and (14)C-cholesterol efflux or LPS- induced cytokine secretion were determined immediately, or after cell resting in culture media alone. The ABCA-1 degradation rate was determined after cell incubation with cycloheximide, and ABCA1 protein level by immunoblot. Oil Red O staining was used to assess intracellular lipid accumulation. Results: A persistent effect of AGE-albumin was observed in macrophages in terms of the secretion of inflammatory cytokines and reduced cholesterol efflux. HDL-mediated (14)C-cholesterol efflux was at least two times higher in macrophages treated with aCG-albumin as compared to bGC-albumin, and intracellular lipid content was significantly reduced in aGC-albumin-treated cells. As compared to bGC-albumin, the ABCA-1 protein content in whole cell bulk was 94% higher in aCG-albumin. A 20% increased ABCA-1 decay rate was observed in macrophages treated with albumin from poorly controlled DM. AGE-albumin has a persistent deleterious effect on macrophage lipid homeostasis and inflammation. The reduction of AGEs in albumin ameliorates cholesterol efflux.
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spelling pubmed-85376112021-10-24 Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages Minanni, Carlos André Machado-Lima, Adriana Iborra, Rodrigo Tallada Okuda, Lígia Shimabukuro de Souza Pinto, Raphael Santana, Monique de Fátima Mello Lira, Aécio Lopes de Araújo Nakandakare, Edna Regina Côrrea-Giannella, Maria Lúcia Cardillo Passarelli, Marisa Nutrients Article Advanced glycated albumin (AGE-albumin) impairs cholesterol efflux and contributes to inflammation in macrophages. The current study evaluated: (1) the persistence of the deleterious effect of AGE-albumin in cholesterol efflux and in inflammation, and (2) how metabolic control in diabetes mellitus (DM) contributes to attenuate the deleterious role of AGE-albumin in macrophage cholesterol homeostasis. Methods: AGE-albumin was produced in vitro or isolated from uncontrolled DM subjects’ serum before (bGC) and after improved glycemic control (aGC). Albumin samples were incubated with bone marrow-derived macrophages and (14)C-cholesterol efflux or LPS- induced cytokine secretion were determined immediately, or after cell resting in culture media alone. The ABCA-1 degradation rate was determined after cell incubation with cycloheximide, and ABCA1 protein level by immunoblot. Oil Red O staining was used to assess intracellular lipid accumulation. Results: A persistent effect of AGE-albumin was observed in macrophages in terms of the secretion of inflammatory cytokines and reduced cholesterol efflux. HDL-mediated (14)C-cholesterol efflux was at least two times higher in macrophages treated with aCG-albumin as compared to bGC-albumin, and intracellular lipid content was significantly reduced in aGC-albumin-treated cells. As compared to bGC-albumin, the ABCA-1 protein content in whole cell bulk was 94% higher in aCG-albumin. A 20% increased ABCA-1 decay rate was observed in macrophages treated with albumin from poorly controlled DM. AGE-albumin has a persistent deleterious effect on macrophage lipid homeostasis and inflammation. The reduction of AGEs in albumin ameliorates cholesterol efflux. MDPI 2021-10-17 /pmc/articles/PMC8537611/ /pubmed/34684632 http://dx.doi.org/10.3390/nu13103633 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Minanni, Carlos André
Machado-Lima, Adriana
Iborra, Rodrigo Tallada
Okuda, Lígia Shimabukuro
de Souza Pinto, Raphael
Santana, Monique de Fátima Mello
Lira, Aécio Lopes de Araújo
Nakandakare, Edna Regina
Côrrea-Giannella, Maria Lúcia Cardillo
Passarelli, Marisa
Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages
title Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages
title_full Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages
title_fullStr Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages
title_full_unstemmed Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages
title_short Persistent Effect of Advanced Glycated Albumin Driving Inflammation and Disturbances in Cholesterol Efflux in Macrophages
title_sort persistent effect of advanced glycated albumin driving inflammation and disturbances in cholesterol efflux in macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537611/
https://www.ncbi.nlm.nih.gov/pubmed/34684632
http://dx.doi.org/10.3390/nu13103633
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