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Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation

The use of cyclodextrins as drug nano-carrier systems for drug delivery is gaining importance in the pharmaceutical industry due to the interesting pharmacokinetic properties of the resulting inclusion complexes. In the present work, complexes of the anti-cancer alkaloids camptothecin and luotonin A...

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Autores principales: González-Ruiz, Víctor, Cores, Ángel, Martín-Cámara, Olmo, Orellana, Karen, Cervera-Carrascón, Víctor, Michalska, Patrycja, Olives, Ana I., León, Rafael, Martín, M. Antonia, Menéndez, J. Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537677/
https://www.ncbi.nlm.nih.gov/pubmed/34683902
http://dx.doi.org/10.3390/pharmaceutics13101609
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author González-Ruiz, Víctor
Cores, Ángel
Martín-Cámara, Olmo
Orellana, Karen
Cervera-Carrascón, Víctor
Michalska, Patrycja
Olives, Ana I.
León, Rafael
Martín, M. Antonia
Menéndez, J. Carlos
author_facet González-Ruiz, Víctor
Cores, Ángel
Martín-Cámara, Olmo
Orellana, Karen
Cervera-Carrascón, Víctor
Michalska, Patrycja
Olives, Ana I.
León, Rafael
Martín, M. Antonia
Menéndez, J. Carlos
author_sort González-Ruiz, Víctor
collection PubMed
description The use of cyclodextrins as drug nano-carrier systems for drug delivery is gaining importance in the pharmaceutical industry due to the interesting pharmacokinetic properties of the resulting inclusion complexes. In the present work, complexes of the anti-cancer alkaloids camptothecin and luotonin A have been prepared with β-cyclodextrin and hydroxypropyl-β-cyclodextrin. These cyclodextrin complexes were characterized by nuclear magnetic resonance spectroscopy (NMR). The variations in the (1)H-NMR and (13)C-NMR chemical shifts allowed to establish the inclusion modes of the compounds into the cyclodextrin cavities, which were supported by docking and molecular dynamics studies. The efficiency of the complexation was quantified by UV-Vis spectrophotometry and spectrofluorimetry, which showed that the protonation equilibria of camptothecin and luotonin A were drastically hampered upon formation of the inclusion complexes. The stabilization of camptothecin towards hydrolysis inside the cyclodextrin cavity was verified by the quantitation of the active lactone form by reverse phase liquid chromatography fluorimetric detection, both in basic conditions and in the presence of serum albumin. The antitumor activity of luotonin A and camptothecin complexes were studied in several cancer cell lines (breast, lung, hepatic carcinoma, ovarian carcinoma and human neuroblastoma) and an enhanced activity was found compared to the free alkaloids, particularly in the case of hydroxypropyl-β-cyclodextrin derivatives. This result shows that the cyclodextrin inclusion strategy has much potential towards reaching the goal of employing luotonin A or its analogues as stable analogues of camptothecin.
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spelling pubmed-85376772021-10-24 Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation González-Ruiz, Víctor Cores, Ángel Martín-Cámara, Olmo Orellana, Karen Cervera-Carrascón, Víctor Michalska, Patrycja Olives, Ana I. León, Rafael Martín, M. Antonia Menéndez, J. Carlos Pharmaceutics Article The use of cyclodextrins as drug nano-carrier systems for drug delivery is gaining importance in the pharmaceutical industry due to the interesting pharmacokinetic properties of the resulting inclusion complexes. In the present work, complexes of the anti-cancer alkaloids camptothecin and luotonin A have been prepared with β-cyclodextrin and hydroxypropyl-β-cyclodextrin. These cyclodextrin complexes were characterized by nuclear magnetic resonance spectroscopy (NMR). The variations in the (1)H-NMR and (13)C-NMR chemical shifts allowed to establish the inclusion modes of the compounds into the cyclodextrin cavities, which were supported by docking and molecular dynamics studies. The efficiency of the complexation was quantified by UV-Vis spectrophotometry and spectrofluorimetry, which showed that the protonation equilibria of camptothecin and luotonin A were drastically hampered upon formation of the inclusion complexes. The stabilization of camptothecin towards hydrolysis inside the cyclodextrin cavity was verified by the quantitation of the active lactone form by reverse phase liquid chromatography fluorimetric detection, both in basic conditions and in the presence of serum albumin. The antitumor activity of luotonin A and camptothecin complexes were studied in several cancer cell lines (breast, lung, hepatic carcinoma, ovarian carcinoma and human neuroblastoma) and an enhanced activity was found compared to the free alkaloids, particularly in the case of hydroxypropyl-β-cyclodextrin derivatives. This result shows that the cyclodextrin inclusion strategy has much potential towards reaching the goal of employing luotonin A or its analogues as stable analogues of camptothecin. MDPI 2021-10-03 /pmc/articles/PMC8537677/ /pubmed/34683902 http://dx.doi.org/10.3390/pharmaceutics13101609 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
González-Ruiz, Víctor
Cores, Ángel
Martín-Cámara, Olmo
Orellana, Karen
Cervera-Carrascón, Víctor
Michalska, Patrycja
Olives, Ana I.
León, Rafael
Martín, M. Antonia
Menéndez, J. Carlos
Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation
title Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation
title_full Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation
title_fullStr Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation
title_full_unstemmed Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation
title_short Enhanced Stability and Bioactivity of Natural Anticancer Topoisomerase I Inhibitors through Cyclodextrin Complexation
title_sort enhanced stability and bioactivity of natural anticancer topoisomerase i inhibitors through cyclodextrin complexation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537677/
https://www.ncbi.nlm.nih.gov/pubmed/34683902
http://dx.doi.org/10.3390/pharmaceutics13101609
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