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Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe

O80:H2 enterohemorrhagic Escherichia coli (EHEC) of sequence type ST301 is one of the main serotypes causing European hemolytic and uremic syndrome, but also invasive infections, due to extra-intestinal virulence factors (VFs). Here, we determined whether other such heteropathotypes exist among ST30...

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Autores principales: Cointe, Aurélie, Bizot, Etienne, Delannoy, Sabine, Fach, Patrick, Bidet, Philippe, Birgy, André, Weill, François-Xavier, Lefèvre, Sophie, Mariani-Kurkdjian, Patricia, Bonacorsi, Stéphane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537712/
https://www.ncbi.nlm.nih.gov/pubmed/34678979
http://dx.doi.org/10.3390/toxins13100686
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author Cointe, Aurélie
Bizot, Etienne
Delannoy, Sabine
Fach, Patrick
Bidet, Philippe
Birgy, André
Weill, François-Xavier
Lefèvre, Sophie
Mariani-Kurkdjian, Patricia
Bonacorsi, Stéphane
author_facet Cointe, Aurélie
Bizot, Etienne
Delannoy, Sabine
Fach, Patrick
Bidet, Philippe
Birgy, André
Weill, François-Xavier
Lefèvre, Sophie
Mariani-Kurkdjian, Patricia
Bonacorsi, Stéphane
author_sort Cointe, Aurélie
collection PubMed
description O80:H2 enterohemorrhagic Escherichia coli (EHEC) of sequence type ST301 is one of the main serotypes causing European hemolytic and uremic syndrome, but also invasive infections, due to extra-intestinal virulence factors (VFs). Here, we determined whether other such heteropathotypes exist among ST301. EnteroBase was screened for ST301 strains that were included in a general SNP-phylogeny. French strains belonging to a new heteropathotype clone were sequenced. ST, hierarchical clusters (HC), serotype, resistome, and virulome were determined using EnteroBase, the CGE website, and local BLAST. The ST301 general phylogeny shows two groups. Group A (n = 25) is mainly composed of enteropathogenic E. coli, whereas group B (n = 55) includes mostly EHEC. Three serotypes, O186:H2, O45:H2 and O55:H9, share the same virulome as one of the O80:H2 sub-clones from which they derive subsequent O-antigen switches. The O55:H9 clone, mainly present in France (n = 29), as well as in the UK (n = 5) and Germany (n = 1), has a low background of genetic diversity (four HC20), although it has three Stx subtypes, an H-antigen switch, and genes encoding the major extra-intestinal VF yersiniabactin, and extended-spectrum beta-lactamases. Diverse heteropathotype clones genetically close to the O80:H2 clone are present among the ST301, requiring close European monitoring, especially the virulent O55:H9 clone.
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spelling pubmed-85377122021-10-24 Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe Cointe, Aurélie Bizot, Etienne Delannoy, Sabine Fach, Patrick Bidet, Philippe Birgy, André Weill, François-Xavier Lefèvre, Sophie Mariani-Kurkdjian, Patricia Bonacorsi, Stéphane Toxins (Basel) Article O80:H2 enterohemorrhagic Escherichia coli (EHEC) of sequence type ST301 is one of the main serotypes causing European hemolytic and uremic syndrome, but also invasive infections, due to extra-intestinal virulence factors (VFs). Here, we determined whether other such heteropathotypes exist among ST301. EnteroBase was screened for ST301 strains that were included in a general SNP-phylogeny. French strains belonging to a new heteropathotype clone were sequenced. ST, hierarchical clusters (HC), serotype, resistome, and virulome were determined using EnteroBase, the CGE website, and local BLAST. The ST301 general phylogeny shows two groups. Group A (n = 25) is mainly composed of enteropathogenic E. coli, whereas group B (n = 55) includes mostly EHEC. Three serotypes, O186:H2, O45:H2 and O55:H9, share the same virulome as one of the O80:H2 sub-clones from which they derive subsequent O-antigen switches. The O55:H9 clone, mainly present in France (n = 29), as well as in the UK (n = 5) and Germany (n = 1), has a low background of genetic diversity (four HC20), although it has three Stx subtypes, an H-antigen switch, and genes encoding the major extra-intestinal VF yersiniabactin, and extended-spectrum beta-lactamases. Diverse heteropathotype clones genetically close to the O80:H2 clone are present among the ST301, requiring close European monitoring, especially the virulent O55:H9 clone. MDPI 2021-09-26 /pmc/articles/PMC8537712/ /pubmed/34678979 http://dx.doi.org/10.3390/toxins13100686 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cointe, Aurélie
Bizot, Etienne
Delannoy, Sabine
Fach, Patrick
Bidet, Philippe
Birgy, André
Weill, François-Xavier
Lefèvre, Sophie
Mariani-Kurkdjian, Patricia
Bonacorsi, Stéphane
Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe
title Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe
title_full Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe
title_fullStr Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe
title_full_unstemmed Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe
title_short Emergence of New ST301 Shiga Toxin-Producing Escherichia coli Clones Harboring Extra-Intestinal Virulence Traits in Europe
title_sort emergence of new st301 shiga toxin-producing escherichia coli clones harboring extra-intestinal virulence traits in europe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537712/
https://www.ncbi.nlm.nih.gov/pubmed/34678979
http://dx.doi.org/10.3390/toxins13100686
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