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Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus
Mycobacterium abscessus (M. abscessus) causes chronic pulmonary infections and is the most difficult non-tuberculous mycobacteria (NTM) to treat due to its resistance to current antimicrobial drugs, with a treatment success rate of 45.6%. Thus, novel treatment drugs are needed, of which we identifie...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537717/ https://www.ncbi.nlm.nih.gov/pubmed/34681686 http://dx.doi.org/10.3390/ijms222011029 |
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author | Lee, Da-Gyum Hwang, Yoo-Hyun Park, Eun-Jin Kim, Jung-Hyun Ryoo, Sung-Weon |
author_facet | Lee, Da-Gyum Hwang, Yoo-Hyun Park, Eun-Jin Kim, Jung-Hyun Ryoo, Sung-Weon |
author_sort | Lee, Da-Gyum |
collection | PubMed |
description | Mycobacterium abscessus (M. abscessus) causes chronic pulmonary infections and is the most difficult non-tuberculous mycobacteria (NTM) to treat due to its resistance to current antimicrobial drugs, with a treatment success rate of 45.6%. Thus, novel treatment drugs are needed, of which we identified the drug clomiphene citrate (CC), known to treat infertility in women, to exhibit inhibitory activity against M. abscessus. To assess the potential of CC as a treatment for M. abscessus pulmonary diseases, we measured its efficacy in vitro and established the intracellular activity of CC against M. abscessus in human macrophages. CC significantly inhibited the growth of not only wild-type M. abscessus strains but also clinical isolate strains and clarithromycin (CLR)-resistant strains of M. abscessus. CC’s drug efficacy did not have cytotoxicity in the infected macrophages. Furthermore, CC worked in anaerobic non-replicating conditions as well as in the presence of biofilm. The results of this in vitro study on M. abscessus activity suggest the possibility of using CC to develop new drug hypotheses for the treatment of M. abscessus infections. |
format | Online Article Text |
id | pubmed-8537717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85377172021-10-24 Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus Lee, Da-Gyum Hwang, Yoo-Hyun Park, Eun-Jin Kim, Jung-Hyun Ryoo, Sung-Weon Int J Mol Sci Article Mycobacterium abscessus (M. abscessus) causes chronic pulmonary infections and is the most difficult non-tuberculous mycobacteria (NTM) to treat due to its resistance to current antimicrobial drugs, with a treatment success rate of 45.6%. Thus, novel treatment drugs are needed, of which we identified the drug clomiphene citrate (CC), known to treat infertility in women, to exhibit inhibitory activity against M. abscessus. To assess the potential of CC as a treatment for M. abscessus pulmonary diseases, we measured its efficacy in vitro and established the intracellular activity of CC against M. abscessus in human macrophages. CC significantly inhibited the growth of not only wild-type M. abscessus strains but also clinical isolate strains and clarithromycin (CLR)-resistant strains of M. abscessus. CC’s drug efficacy did not have cytotoxicity in the infected macrophages. Furthermore, CC worked in anaerobic non-replicating conditions as well as in the presence of biofilm. The results of this in vitro study on M. abscessus activity suggest the possibility of using CC to develop new drug hypotheses for the treatment of M. abscessus infections. MDPI 2021-10-13 /pmc/articles/PMC8537717/ /pubmed/34681686 http://dx.doi.org/10.3390/ijms222011029 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Da-Gyum Hwang, Yoo-Hyun Park, Eun-Jin Kim, Jung-Hyun Ryoo, Sung-Weon Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus |
title | Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus |
title_full | Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus |
title_fullStr | Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus |
title_full_unstemmed | Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus |
title_short | Clomiphene Citrate Shows Effective and Sustained Antimicrobial Activity against Mycobacterium abscessus |
title_sort | clomiphene citrate shows effective and sustained antimicrobial activity against mycobacterium abscessus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537717/ https://www.ncbi.nlm.nih.gov/pubmed/34681686 http://dx.doi.org/10.3390/ijms222011029 |
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