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Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus

Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Bra...

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Autores principales: Liu, Zhiquan, Li, Bing, Yang, Yong, Gao, Changbin, Yi, Bin, Wen, Jing, Shen, Jinxiong, Tu, Jinxing, Fu, Tingdong, Dai, Cheng, Ma, Chaozhi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537745/
https://www.ncbi.nlm.nih.gov/pubmed/34685996
http://dx.doi.org/10.3390/plants10102186
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author Liu, Zhiquan
Li, Bing
Yang, Yong
Gao, Changbin
Yi, Bin
Wen, Jing
Shen, Jinxiong
Tu, Jinxing
Fu, Tingdong
Dai, Cheng
Ma, Chaozhi
author_facet Liu, Zhiquan
Li, Bing
Yang, Yong
Gao, Changbin
Yi, Bin
Wen, Jing
Shen, Jinxiong
Tu, Jinxing
Fu, Tingdong
Dai, Cheng
Ma, Chaozhi
author_sort Liu, Zhiquan
collection PubMed
description Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus.
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spelling pubmed-85377452021-10-24 Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus Liu, Zhiquan Li, Bing Yang, Yong Gao, Changbin Yi, Bin Wen, Jing Shen, Jinxiong Tu, Jinxing Fu, Tingdong Dai, Cheng Ma, Chaozhi Plants (Basel) Article Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus. MDPI 2021-10-15 /pmc/articles/PMC8537745/ /pubmed/34685996 http://dx.doi.org/10.3390/plants10102186 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Zhiquan
Li, Bing
Yang, Yong
Gao, Changbin
Yi, Bin
Wen, Jing
Shen, Jinxiong
Tu, Jinxing
Fu, Tingdong
Dai, Cheng
Ma, Chaozhi
Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus
title Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus
title_full Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus
title_fullStr Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus
title_full_unstemmed Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus
title_short Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus
title_sort characterization of a common s haplotype bns-6 in the self-incompatibility of brassica napus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537745/
https://www.ncbi.nlm.nih.gov/pubmed/34685996
http://dx.doi.org/10.3390/plants10102186
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