Cargando…
Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus
Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Bra...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537745/ https://www.ncbi.nlm.nih.gov/pubmed/34685996 http://dx.doi.org/10.3390/plants10102186 |
_version_ | 1784588335248834560 |
---|---|
author | Liu, Zhiquan Li, Bing Yang, Yong Gao, Changbin Yi, Bin Wen, Jing Shen, Jinxiong Tu, Jinxing Fu, Tingdong Dai, Cheng Ma, Chaozhi |
author_facet | Liu, Zhiquan Li, Bing Yang, Yong Gao, Changbin Yi, Bin Wen, Jing Shen, Jinxiong Tu, Jinxing Fu, Tingdong Dai, Cheng Ma, Chaozhi |
author_sort | Liu, Zhiquan |
collection | PubMed |
description | Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus. |
format | Online Article Text |
id | pubmed-8537745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85377452021-10-24 Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus Liu, Zhiquan Li, Bing Yang, Yong Gao, Changbin Yi, Bin Wen, Jing Shen, Jinxiong Tu, Jinxing Fu, Tingdong Dai, Cheng Ma, Chaozhi Plants (Basel) Article Self-incompatibility (SI) is a pollen-stigma recognition system controlled by a single and highly polymorphic genetic locus known as the S-locus. The S-locus exists in all Brassica napus (B. napus, AACC), but natural B. napus accessions are self-compatible. About 100 and 50 S haplotypes exist in Brassica rapa (AA) and Brassica oleracea (CC), respectively. However, S haplotypes have not been detected in B. napus populations. In this study, we detected the S haplotype distribution in B. napus and ascertained the function of a common S haplotype BnS-6 through genetic transformation. BnS-1/BnS-6 and BnS-7/BnS-6 were the main S haplotypes in 523 B. napus cultivars and inbred lines. The expression of SRK in different S haplotypes was normal (the expression of SCR in the A subgenome affected the SI phenotype) while the expression of BnSCR-6 in the C subgenome had no correlation with the SI phenotype in B. napus. The BnSCR-6 protein in BnSCR-6 overexpressed lines was functional, but the self-compatibility of overexpressed lines did not change. The low expression of BnSCR-6 could be a reason for the inactivation of BnS-6 in the SI response of B. napus. This study lays a foundation for research on the self-compatibility mechanism and the SI-related breeding in B. napus. MDPI 2021-10-15 /pmc/articles/PMC8537745/ /pubmed/34685996 http://dx.doi.org/10.3390/plants10102186 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Zhiquan Li, Bing Yang, Yong Gao, Changbin Yi, Bin Wen, Jing Shen, Jinxiong Tu, Jinxing Fu, Tingdong Dai, Cheng Ma, Chaozhi Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus |
title | Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus |
title_full | Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus |
title_fullStr | Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus |
title_full_unstemmed | Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus |
title_short | Characterization of a Common S Haplotype BnS-6 in the Self-Incompatibility of Brassica napus |
title_sort | characterization of a common s haplotype bns-6 in the self-incompatibility of brassica napus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537745/ https://www.ncbi.nlm.nih.gov/pubmed/34685996 http://dx.doi.org/10.3390/plants10102186 |
work_keys_str_mv | AT liuzhiquan characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT libing characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT yangyong characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT gaochangbin characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT yibin characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT wenjing characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT shenjinxiong characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT tujinxing characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT futingdong characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT daicheng characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus AT machaozhi characterizationofacommonshaplotypebns6intheselfincompatibilityofbrassicanapus |