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Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations

Hepatitis E virus (HEV) infection can lead to a variety of neurological disorders. While HEV RNA is known to be present in the central nervous system, HEV quasispecies in serum and cerebrospinal fluid (CSF) have rarely been explored. We studied the virus’ quasispecies in the blood and the CSF of fiv...

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Autores principales: Abravanel, Florence, Nicot, Florence, Lhomme, Sébastien, Cazabat, Michele, Drumel, Thomas, Velay, Aurélie, Latour, Justine, Belliere, Julie, Cintas, Pascal, Kamar, Nassim, Izopet, Jacques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537826/
https://www.ncbi.nlm.nih.gov/pubmed/34696313
http://dx.doi.org/10.3390/vaccines9101205
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author Abravanel, Florence
Nicot, Florence
Lhomme, Sébastien
Cazabat, Michele
Drumel, Thomas
Velay, Aurélie
Latour, Justine
Belliere, Julie
Cintas, Pascal
Kamar, Nassim
Izopet, Jacques
author_facet Abravanel, Florence
Nicot, Florence
Lhomme, Sébastien
Cazabat, Michele
Drumel, Thomas
Velay, Aurélie
Latour, Justine
Belliere, Julie
Cintas, Pascal
Kamar, Nassim
Izopet, Jacques
author_sort Abravanel, Florence
collection PubMed
description Hepatitis E virus (HEV) infection can lead to a variety of neurological disorders. While HEV RNA is known to be present in the central nervous system, HEV quasispecies in serum and cerebrospinal fluid (CSF) have rarely been explored. We studied the virus’ quasispecies in the blood and the CSF of five patients at the onset of their neurological symptoms. The samples of three patients suffering from meningitis, neuralgic amyotrophy and acute inflammatory polyradiculoneuropathy were taken at the acute phase of the HEV infection. The samples from the other two patients were taken during the chronic phase (5 years after HEV diagnosis) when they presented with clinical signs of encephalitis. We sequenced at least 20 randomly polyproline regions of the selected virus clones. Phylogenetic analysis of the virus variants in the blood and the CSF revealed no virus compartmentalization for the three acute-phase patients but there was clear evidence of HEV quasispecies compartmentalization in the CSF of the two patients during chronic infection. In conclusion, prolonged infection in the immunocompromised condition can lead to independent virus replication in the liver and the tissues, producing viruses in CSF.
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spelling pubmed-85378262021-10-24 Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations Abravanel, Florence Nicot, Florence Lhomme, Sébastien Cazabat, Michele Drumel, Thomas Velay, Aurélie Latour, Justine Belliere, Julie Cintas, Pascal Kamar, Nassim Izopet, Jacques Vaccines (Basel) Article Hepatitis E virus (HEV) infection can lead to a variety of neurological disorders. While HEV RNA is known to be present in the central nervous system, HEV quasispecies in serum and cerebrospinal fluid (CSF) have rarely been explored. We studied the virus’ quasispecies in the blood and the CSF of five patients at the onset of their neurological symptoms. The samples of three patients suffering from meningitis, neuralgic amyotrophy and acute inflammatory polyradiculoneuropathy were taken at the acute phase of the HEV infection. The samples from the other two patients were taken during the chronic phase (5 years after HEV diagnosis) when they presented with clinical signs of encephalitis. We sequenced at least 20 randomly polyproline regions of the selected virus clones. Phylogenetic analysis of the virus variants in the blood and the CSF revealed no virus compartmentalization for the three acute-phase patients but there was clear evidence of HEV quasispecies compartmentalization in the CSF of the two patients during chronic infection. In conclusion, prolonged infection in the immunocompromised condition can lead to independent virus replication in the liver and the tissues, producing viruses in CSF. MDPI 2021-10-19 /pmc/articles/PMC8537826/ /pubmed/34696313 http://dx.doi.org/10.3390/vaccines9101205 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Abravanel, Florence
Nicot, Florence
Lhomme, Sébastien
Cazabat, Michele
Drumel, Thomas
Velay, Aurélie
Latour, Justine
Belliere, Julie
Cintas, Pascal
Kamar, Nassim
Izopet, Jacques
Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations
title Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations
title_full Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations
title_fullStr Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations
title_full_unstemmed Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations
title_short Hepatitis E Virus Quasispecies in Cerebrospinal Fluid with Neurological Manifestations
title_sort hepatitis e virus quasispecies in cerebrospinal fluid with neurological manifestations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537826/
https://www.ncbi.nlm.nih.gov/pubmed/34696313
http://dx.doi.org/10.3390/vaccines9101205
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