Cargando…

Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station

The establishment of steady-state continuous crop production during long-term deep space missions is critical for providing consistent nutritional and psychological benefits for the crew, potentially improving their health and performance. Three technology demonstrations were completed achieving sim...

Descripción completa

Detalles Bibliográficos
Autores principales: Hummerick, Mary E., Khodadad, Christina L. M., Dixit, Anirudha R., Spencer, Lashelle E., Maldonado-Vasquez, Gretchen J., Gooden, Jennifer L., Spern, Cory J., Fischer, Jason A., Dufour, Nicole, Wheeler, Raymond M., Romeyn, Matthew W., Smith, Trent M., Massa, Gioia D., Zhang, Ye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537831/
https://www.ncbi.nlm.nih.gov/pubmed/34685431
http://dx.doi.org/10.3390/life11101060
_version_ 1784588356464672768
author Hummerick, Mary E.
Khodadad, Christina L. M.
Dixit, Anirudha R.
Spencer, Lashelle E.
Maldonado-Vasquez, Gretchen J.
Gooden, Jennifer L.
Spern, Cory J.
Fischer, Jason A.
Dufour, Nicole
Wheeler, Raymond M.
Romeyn, Matthew W.
Smith, Trent M.
Massa, Gioia D.
Zhang, Ye
author_facet Hummerick, Mary E.
Khodadad, Christina L. M.
Dixit, Anirudha R.
Spencer, Lashelle E.
Maldonado-Vasquez, Gretchen J.
Gooden, Jennifer L.
Spern, Cory J.
Fischer, Jason A.
Dufour, Nicole
Wheeler, Raymond M.
Romeyn, Matthew W.
Smith, Trent M.
Massa, Gioia D.
Zhang, Ye
author_sort Hummerick, Mary E.
collection PubMed
description The establishment of steady-state continuous crop production during long-term deep space missions is critical for providing consistent nutritional and psychological benefits for the crew, potentially improving their health and performance. Three technology demonstrations were completed achieving simultaneous multi-species plant growth and the concurrent use of two Veggie units on the International Space Station (ISS). Microbiological characterization using molecular and culture-based methods was performed on leaves and roots from two harvests of three leafy greens, red romaine lettuce (Lactuca sativa cv. ‘Outredgeous’); mizuna mustard, (Brassica rapa var japonica); and green leaf lettuce, (Lactuca sativa cv. Waldmann’s) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption. Surface samples of the Veggie facility and plant pillows revealed low counts of bacteria and fungi and are commonly isolated on ISS. Community analysis was completed with 16S rRNA amplicon sequencing. Comparisons between pillow components, and plant tissue types from VEG-03D, E, and F revealed higher diversity in roots and rooting substrate than the leaves and wick. This work provides valuable information for food production-related research on the ISS and the impact of the plant microbiome on this unique closed environment.
format Online
Article
Text
id pubmed-8537831
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85378312021-10-24 Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station Hummerick, Mary E. Khodadad, Christina L. M. Dixit, Anirudha R. Spencer, Lashelle E. Maldonado-Vasquez, Gretchen J. Gooden, Jennifer L. Spern, Cory J. Fischer, Jason A. Dufour, Nicole Wheeler, Raymond M. Romeyn, Matthew W. Smith, Trent M. Massa, Gioia D. Zhang, Ye Life (Basel) Article The establishment of steady-state continuous crop production during long-term deep space missions is critical for providing consistent nutritional and psychological benefits for the crew, potentially improving their health and performance. Three technology demonstrations were completed achieving simultaneous multi-species plant growth and the concurrent use of two Veggie units on the International Space Station (ISS). Microbiological characterization using molecular and culture-based methods was performed on leaves and roots from two harvests of three leafy greens, red romaine lettuce (Lactuca sativa cv. ‘Outredgeous’); mizuna mustard, (Brassica rapa var japonica); and green leaf lettuce, (Lactuca sativa cv. Waldmann’s) and associated rooting pillow components and Veggie chamber surfaces. Culture based enumeration and pathogen screening indicated the leafy greens were safe for consumption. Surface samples of the Veggie facility and plant pillows revealed low counts of bacteria and fungi and are commonly isolated on ISS. Community analysis was completed with 16S rRNA amplicon sequencing. Comparisons between pillow components, and plant tissue types from VEG-03D, E, and F revealed higher diversity in roots and rooting substrate than the leaves and wick. This work provides valuable information for food production-related research on the ISS and the impact of the plant microbiome on this unique closed environment. MDPI 2021-10-09 /pmc/articles/PMC8537831/ /pubmed/34685431 http://dx.doi.org/10.3390/life11101060 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hummerick, Mary E.
Khodadad, Christina L. M.
Dixit, Anirudha R.
Spencer, Lashelle E.
Maldonado-Vasquez, Gretchen J.
Gooden, Jennifer L.
Spern, Cory J.
Fischer, Jason A.
Dufour, Nicole
Wheeler, Raymond M.
Romeyn, Matthew W.
Smith, Trent M.
Massa, Gioia D.
Zhang, Ye
Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station
title Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station
title_full Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station
title_fullStr Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station
title_full_unstemmed Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station
title_short Spatial Characterization of Microbial Communities on Multi-Species Leafy Greens Grown Simultaneously in the Vegetable Production Systems on the International Space Station
title_sort spatial characterization of microbial communities on multi-species leafy greens grown simultaneously in the vegetable production systems on the international space station
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537831/
https://www.ncbi.nlm.nih.gov/pubmed/34685431
http://dx.doi.org/10.3390/life11101060
work_keys_str_mv AT hummerickmarye spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT khodadadchristinalm spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT dixitanirudhar spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT spencerlashellee spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT maldonadovasquezgretchenj spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT goodenjenniferl spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT sperncoryj spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT fischerjasona spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT dufournicole spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT wheelerraymondm spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT romeynmattheww spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT smithtrentm spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT massagioiad spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation
AT zhangye spatialcharacterizationofmicrobialcommunitiesonmultispeciesleafygreensgrownsimultaneouslyinthevegetableproductionsystemsontheinternationalspacestation