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Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells

Type 2 diabetes and obesity are major problems worldwide and dietary polyphenols have shown efficacy to ameliorate signs of these diseases. Anthocyanins from berries display potent antioxidants and protect against weight gain and insulin resistance in different models of diet-induced metabolic syndr...

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Autores principales: del Campo, Andrea, Salamanca, Catalina, Fajardo, Angelo, Díaz-Castro, Francisco, Bustos, Catalina, Calfío, Camila, Troncoso, Rodrigo, Pastene-Navarrete, Edgar R., Acuna-Castillo, Claudio, Milla, Luis A., Villarroel, Carlos A., Cubillos, Francisco A., Aranda, Mario, Rojo, Leonel E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537850/
https://www.ncbi.nlm.nih.gov/pubmed/34684731
http://dx.doi.org/10.3390/molecules26206149
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author del Campo, Andrea
Salamanca, Catalina
Fajardo, Angelo
Díaz-Castro, Francisco
Bustos, Catalina
Calfío, Camila
Troncoso, Rodrigo
Pastene-Navarrete, Edgar R.
Acuna-Castillo, Claudio
Milla, Luis A.
Villarroel, Carlos A.
Cubillos, Francisco A.
Aranda, Mario
Rojo, Leonel E.
author_facet del Campo, Andrea
Salamanca, Catalina
Fajardo, Angelo
Díaz-Castro, Francisco
Bustos, Catalina
Calfío, Camila
Troncoso, Rodrigo
Pastene-Navarrete, Edgar R.
Acuna-Castillo, Claudio
Milla, Luis A.
Villarroel, Carlos A.
Cubillos, Francisco A.
Aranda, Mario
Rojo, Leonel E.
author_sort del Campo, Andrea
collection PubMed
description Type 2 diabetes and obesity are major problems worldwide and dietary polyphenols have shown efficacy to ameliorate signs of these diseases. Anthocyanins from berries display potent antioxidants and protect against weight gain and insulin resistance in different models of diet-induced metabolic syndrome. Olanzapine is known to induce an accelerated form of metabolic syndrome. Due to the aforementioned, we evaluated whether delphinidin-3,5-O-diglucoside (DG) and delphinidin-3-O-sambubioside-5-O-glucoside (DS), two potent antidiabetic anthocyanins isolated from Aristotelia chilensis fruit, could prevent olanzapine-induced steatosis and insulin resistance in liver and skeletal muscle cells, respectively. HepG2 liver cells and L6 skeletal muscle cells were co-incubated with DG 50 μg/mL or DS 50 μg/mL plus olanzapine 50 μg/mL. Lipid accumulation was determined in HepG2 cells while the expression of p-Akt as a key regulator of the insulin-activated signaling pathways, mitochondrial function, and glucose uptake was assessed in L6 cells. DS and DG prevented olanzapine-induced lipid accumulation in liver cells. However, insulin signaling impairment induced by olanzapine in L6 cells was not rescued by DS and DG. Thus, anthocyanins modulate lipid metabolism, which is a relevant factor in hepatic tissue, but do not significantly influence skeletal muscle, where a potent antioxidant effect of olanzapine was found.
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spelling pubmed-85378502021-10-24 Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells del Campo, Andrea Salamanca, Catalina Fajardo, Angelo Díaz-Castro, Francisco Bustos, Catalina Calfío, Camila Troncoso, Rodrigo Pastene-Navarrete, Edgar R. Acuna-Castillo, Claudio Milla, Luis A. Villarroel, Carlos A. Cubillos, Francisco A. Aranda, Mario Rojo, Leonel E. Molecules Article Type 2 diabetes and obesity are major problems worldwide and dietary polyphenols have shown efficacy to ameliorate signs of these diseases. Anthocyanins from berries display potent antioxidants and protect against weight gain and insulin resistance in different models of diet-induced metabolic syndrome. Olanzapine is known to induce an accelerated form of metabolic syndrome. Due to the aforementioned, we evaluated whether delphinidin-3,5-O-diglucoside (DG) and delphinidin-3-O-sambubioside-5-O-glucoside (DS), two potent antidiabetic anthocyanins isolated from Aristotelia chilensis fruit, could prevent olanzapine-induced steatosis and insulin resistance in liver and skeletal muscle cells, respectively. HepG2 liver cells and L6 skeletal muscle cells were co-incubated with DG 50 μg/mL or DS 50 μg/mL plus olanzapine 50 μg/mL. Lipid accumulation was determined in HepG2 cells while the expression of p-Akt as a key regulator of the insulin-activated signaling pathways, mitochondrial function, and glucose uptake was assessed in L6 cells. DS and DG prevented olanzapine-induced lipid accumulation in liver cells. However, insulin signaling impairment induced by olanzapine in L6 cells was not rescued by DS and DG. Thus, anthocyanins modulate lipid metabolism, which is a relevant factor in hepatic tissue, but do not significantly influence skeletal muscle, where a potent antioxidant effect of olanzapine was found. MDPI 2021-10-12 /pmc/articles/PMC8537850/ /pubmed/34684731 http://dx.doi.org/10.3390/molecules26206149 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
del Campo, Andrea
Salamanca, Catalina
Fajardo, Angelo
Díaz-Castro, Francisco
Bustos, Catalina
Calfío, Camila
Troncoso, Rodrigo
Pastene-Navarrete, Edgar R.
Acuna-Castillo, Claudio
Milla, Luis A.
Villarroel, Carlos A.
Cubillos, Francisco A.
Aranda, Mario
Rojo, Leonel E.
Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells
title Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells
title_full Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells
title_fullStr Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells
title_full_unstemmed Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells
title_short Anthocyanins from Aristotelia chilensis Prevent Olanzapine-Induced Hepatic-Lipid Accumulation but Not Insulin Resistance in Skeletal Muscle Cells
title_sort anthocyanins from aristotelia chilensis prevent olanzapine-induced hepatic-lipid accumulation but not insulin resistance in skeletal muscle cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537850/
https://www.ncbi.nlm.nih.gov/pubmed/34684731
http://dx.doi.org/10.3390/molecules26206149
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