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Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging
Various cell therapy strategies, including chimeric antigen receptor-expressing T or natural killer (NK) cells and cell-mediated drug delivery, have been developed for tumor eradication. However, the efficiency of these strategies against solid tumors remains unclear. We hypothesized that real-time...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537855/ https://www.ncbi.nlm.nih.gov/pubmed/34683982 http://dx.doi.org/10.3390/pharmaceutics13101689 |
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author | Song, Hyeong-Woo Lee, Han-Sol Kim, Seok-Jae Kim, Ho Yong Choi, You Hee Kang, Byungjeon Kim, Chang-Sei Park, Jong-Oh Choi, Eunpyo |
author_facet | Song, Hyeong-Woo Lee, Han-Sol Kim, Seok-Jae Kim, Ho Yong Choi, You Hee Kang, Byungjeon Kim, Chang-Sei Park, Jong-Oh Choi, Eunpyo |
author_sort | Song, Hyeong-Woo |
collection | PubMed |
description | Various cell therapy strategies, including chimeric antigen receptor-expressing T or natural killer (NK) cells and cell-mediated drug delivery, have been developed for tumor eradication. However, the efficiency of these strategies against solid tumors remains unclear. We hypothesized that real-time control and visualization of therapeutic cells, such as NK cells, would improve their therapeutic efficacy against solid tumors. In this study, we engineered Sonazoid microbubble-conjugated NK (NK_Sona) cells and demonstrated that they were detectable by ultrasound imaging in real-time and maintained their functions. The Sonazoid microbubbles on the cell membrane did not affect the cytotoxicity and viability of the NK cells in vitro. Additionally, the NK_Sona cells could be visualized by ultrasound imaging and inhibited tumor growth in vivo. Taken together, our findings demonstrate the feasibility of this new approach in the use of therapeutic cells, such as NK cells, against solid tumors. |
format | Online Article Text |
id | pubmed-8537855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85378552021-10-24 Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging Song, Hyeong-Woo Lee, Han-Sol Kim, Seok-Jae Kim, Ho Yong Choi, You Hee Kang, Byungjeon Kim, Chang-Sei Park, Jong-Oh Choi, Eunpyo Pharmaceutics Article Various cell therapy strategies, including chimeric antigen receptor-expressing T or natural killer (NK) cells and cell-mediated drug delivery, have been developed for tumor eradication. However, the efficiency of these strategies against solid tumors remains unclear. We hypothesized that real-time control and visualization of therapeutic cells, such as NK cells, would improve their therapeutic efficacy against solid tumors. In this study, we engineered Sonazoid microbubble-conjugated NK (NK_Sona) cells and demonstrated that they were detectable by ultrasound imaging in real-time and maintained their functions. The Sonazoid microbubbles on the cell membrane did not affect the cytotoxicity and viability of the NK cells in vitro. Additionally, the NK_Sona cells could be visualized by ultrasound imaging and inhibited tumor growth in vivo. Taken together, our findings demonstrate the feasibility of this new approach in the use of therapeutic cells, such as NK cells, against solid tumors. MDPI 2021-10-15 /pmc/articles/PMC8537855/ /pubmed/34683982 http://dx.doi.org/10.3390/pharmaceutics13101689 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Song, Hyeong-Woo Lee, Han-Sol Kim, Seok-Jae Kim, Ho Yong Choi, You Hee Kang, Byungjeon Kim, Chang-Sei Park, Jong-Oh Choi, Eunpyo Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging |
title | Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging |
title_full | Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging |
title_fullStr | Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging |
title_full_unstemmed | Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging |
title_short | Sonazoid-Conjugated Natural Killer Cells for Tumor Therapy and Real-Time Visualization by Ultrasound Imaging |
title_sort | sonazoid-conjugated natural killer cells for tumor therapy and real-time visualization by ultrasound imaging |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8537855/ https://www.ncbi.nlm.nih.gov/pubmed/34683982 http://dx.doi.org/10.3390/pharmaceutics13101689 |
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