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Telocytes and Lymphatics of the Human Colon
Background: Telocytes (TCs) are a peculiar morphological type of stromal cells. They project long and moniliform telopodes, visible on various bidimensional sections. Originally regarded as “interstitial Cajal-like cells”, gastrointestinal TCs were CD34+. Further double-labelling studies found that...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538068/ https://www.ncbi.nlm.nih.gov/pubmed/34685373 http://dx.doi.org/10.3390/life11101001 |
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author | Zurzu, Mihai Nicolescu, Mihnea Ioan Mogoantă, Laurențiu Pantea, Stelian Rusu, Mugurel Constantin |
author_facet | Zurzu, Mihai Nicolescu, Mihnea Ioan Mogoantă, Laurențiu Pantea, Stelian Rusu, Mugurel Constantin |
author_sort | Zurzu, Mihai |
collection | PubMed |
description | Background: Telocytes (TCs) are a peculiar morphological type of stromal cells. They project long and moniliform telopodes, visible on various bidimensional sections. Originally regarded as “interstitial Cajal-like cells”, gastrointestinal TCs were CD34+. Further double-labelling studies found that colon TCs are negative for the expressions of the PDGFR-α and α-SMA. However, the TCs in colon were not distinguished specifically from endothelial cells (ECs), vascular or lymphatic. A combinational approach is important for accurate TC identification. Hence, we designed an immunohistochemical study of human colon to check whether ECs and CD34+ TCs express different markers. Methods: Immunohistochemistry was performed on archived paraffin-embedded samples of human colon (nine cases) for the following markers: CD31, CD34, CD117/c-kit and D2-40 (podoplanin). Results: A distinctive population of CD34+ TCs was found coating the myenteric ganglia. However, also perivascular cells and vascular ECs were CD34+. c-kit expression was equally found in interstitial Cajal cells (ICCs) and perivascular cells. The CD34 TCs did not express c-kit. As they were equally CD31- and D2-40- they were assessed as different from ECs. Conclusions: Testing specific markers of ECs, vascular and lymphatic, in the same tissues in which CD34+ TCs are found, is much more relevant than to identify TCs by transmission electron microscopy alone. |
format | Online Article Text |
id | pubmed-8538068 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85380682021-10-24 Telocytes and Lymphatics of the Human Colon Zurzu, Mihai Nicolescu, Mihnea Ioan Mogoantă, Laurențiu Pantea, Stelian Rusu, Mugurel Constantin Life (Basel) Article Background: Telocytes (TCs) are a peculiar morphological type of stromal cells. They project long and moniliform telopodes, visible on various bidimensional sections. Originally regarded as “interstitial Cajal-like cells”, gastrointestinal TCs were CD34+. Further double-labelling studies found that colon TCs are negative for the expressions of the PDGFR-α and α-SMA. However, the TCs in colon were not distinguished specifically from endothelial cells (ECs), vascular or lymphatic. A combinational approach is important for accurate TC identification. Hence, we designed an immunohistochemical study of human colon to check whether ECs and CD34+ TCs express different markers. Methods: Immunohistochemistry was performed on archived paraffin-embedded samples of human colon (nine cases) for the following markers: CD31, CD34, CD117/c-kit and D2-40 (podoplanin). Results: A distinctive population of CD34+ TCs was found coating the myenteric ganglia. However, also perivascular cells and vascular ECs were CD34+. c-kit expression was equally found in interstitial Cajal cells (ICCs) and perivascular cells. The CD34 TCs did not express c-kit. As they were equally CD31- and D2-40- they were assessed as different from ECs. Conclusions: Testing specific markers of ECs, vascular and lymphatic, in the same tissues in which CD34+ TCs are found, is much more relevant than to identify TCs by transmission electron microscopy alone. MDPI 2021-09-23 /pmc/articles/PMC8538068/ /pubmed/34685373 http://dx.doi.org/10.3390/life11101001 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zurzu, Mihai Nicolescu, Mihnea Ioan Mogoantă, Laurențiu Pantea, Stelian Rusu, Mugurel Constantin Telocytes and Lymphatics of the Human Colon |
title | Telocytes and Lymphatics of the Human Colon |
title_full | Telocytes and Lymphatics of the Human Colon |
title_fullStr | Telocytes and Lymphatics of the Human Colon |
title_full_unstemmed | Telocytes and Lymphatics of the Human Colon |
title_short | Telocytes and Lymphatics of the Human Colon |
title_sort | telocytes and lymphatics of the human colon |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538068/ https://www.ncbi.nlm.nih.gov/pubmed/34685373 http://dx.doi.org/10.3390/life11101001 |
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