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Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance

As the incidence of obesity and type 2 diabetes (T2D) is occurring at a younger age, studying adolescent nutrient metabolism can provide insights on the development of T2D. Metabolic challenges, including an oral glucose tolerance test (OGTT) can assess the effects of perturbations in nutrient metab...

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Autores principales: LaBarre, Jennifer L., Hirschfeld, Emily, Soni, Tanu, Kachman, Maureen, Wigginton, Janis, Duren, William, Fleischman, Johanna Y., Karnovsky, Alla, Burant, Charles F., Lee, Joyce M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538092/
https://www.ncbi.nlm.nih.gov/pubmed/34684365
http://dx.doi.org/10.3390/nu13103365
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author LaBarre, Jennifer L.
Hirschfeld, Emily
Soni, Tanu
Kachman, Maureen
Wigginton, Janis
Duren, William
Fleischman, Johanna Y.
Karnovsky, Alla
Burant, Charles F.
Lee, Joyce M.
author_facet LaBarre, Jennifer L.
Hirschfeld, Emily
Soni, Tanu
Kachman, Maureen
Wigginton, Janis
Duren, William
Fleischman, Johanna Y.
Karnovsky, Alla
Burant, Charles F.
Lee, Joyce M.
author_sort LaBarre, Jennifer L.
collection PubMed
description As the incidence of obesity and type 2 diabetes (T2D) is occurring at a younger age, studying adolescent nutrient metabolism can provide insights on the development of T2D. Metabolic challenges, including an oral glucose tolerance test (OGTT) can assess the effects of perturbations in nutrient metabolism. Here, we present alterations in the global metabolome in response to an OGTT, classifying the influence of obesity and insulin resistance (IR) in adolescents that arrived at the clinic fasted and in a random-fed state. Participants were recruited as lean (n = 55, aged 8–17 years, BMI percentile 5–85%) and overweight and obese (OVOB, n = 228, aged 8–17 years, BMI percentile ≥ 85%). Untargeted metabolomics profiled 246 annotated metabolites in plasma at t0 and t60 min during the OGTT. Our results suggest that obesity and IR influence the switch from fatty acid (FA) to glucose oxidation in response to the OGTT. Obesity was associated with a blunted decline of acylcarnitines and fatty acid oxidation intermediates. In females, metabolites from the Fasted and Random-Fed OGTT were associated with HOMA-IR, including diacylglycerols, leucine/isoleucine, acylcarnitines, and phosphocholines. Our results indicate that at an early age, obesity and IR may influence the metabolome dynamics in response to a glucose challenge.
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spelling pubmed-85380922021-10-24 Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance LaBarre, Jennifer L. Hirschfeld, Emily Soni, Tanu Kachman, Maureen Wigginton, Janis Duren, William Fleischman, Johanna Y. Karnovsky, Alla Burant, Charles F. Lee, Joyce M. Nutrients Article As the incidence of obesity and type 2 diabetes (T2D) is occurring at a younger age, studying adolescent nutrient metabolism can provide insights on the development of T2D. Metabolic challenges, including an oral glucose tolerance test (OGTT) can assess the effects of perturbations in nutrient metabolism. Here, we present alterations in the global metabolome in response to an OGTT, classifying the influence of obesity and insulin resistance (IR) in adolescents that arrived at the clinic fasted and in a random-fed state. Participants were recruited as lean (n = 55, aged 8–17 years, BMI percentile 5–85%) and overweight and obese (OVOB, n = 228, aged 8–17 years, BMI percentile ≥ 85%). Untargeted metabolomics profiled 246 annotated metabolites in plasma at t0 and t60 min during the OGTT. Our results suggest that obesity and IR influence the switch from fatty acid (FA) to glucose oxidation in response to the OGTT. Obesity was associated with a blunted decline of acylcarnitines and fatty acid oxidation intermediates. In females, metabolites from the Fasted and Random-Fed OGTT were associated with HOMA-IR, including diacylglycerols, leucine/isoleucine, acylcarnitines, and phosphocholines. Our results indicate that at an early age, obesity and IR may influence the metabolome dynamics in response to a glucose challenge. MDPI 2021-09-25 /pmc/articles/PMC8538092/ /pubmed/34684365 http://dx.doi.org/10.3390/nu13103365 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
LaBarre, Jennifer L.
Hirschfeld, Emily
Soni, Tanu
Kachman, Maureen
Wigginton, Janis
Duren, William
Fleischman, Johanna Y.
Karnovsky, Alla
Burant, Charles F.
Lee, Joyce M.
Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance
title Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance
title_full Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance
title_fullStr Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance
title_full_unstemmed Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance
title_short Comparing the Fasting and Random-Fed Metabolome Response to an Oral Glucose Tolerance Test in Children and Adolescents: Implications of Sex, Obesity, and Insulin Resistance
title_sort comparing the fasting and random-fed metabolome response to an oral glucose tolerance test in children and adolescents: implications of sex, obesity, and insulin resistance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538092/
https://www.ncbi.nlm.nih.gov/pubmed/34684365
http://dx.doi.org/10.3390/nu13103365
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