Preferential Attraction of Oviposition-Ready Oriental Fruit Flies to Host Fruit Odor over Protein Food Odor

SIMPLE SUMMARY: Oriental fruit fly, Bactrocera dorsalis, is one of the most destructive invasive pests of tropical fruit and vegetable crops worldwide. Current oriental fruit fly quarantine programs focus heavily on the control and surveillance of male flies, which is less effective for mitigating the impact caused by female populations. We investigated the relationship between olfactory preference and oviposition outcome of oriental fruit flies. In laboratory bioassays using similarly aged (14–16 day old) cohorts of mated females, some females preferred host fruit odors over protein food odor (torula yeast), while some preferred protein odor. The females that preferred host fruit odor had 2.1 times greater egg load and laid 2.4 times more eggs than females that preferred protein odor. Our results suggest that mated female oriental fruit flies with a preference for host fruit odor are likely to be in an imminent oviposition-ready physiological status, while mated females that prefer torula yeast odor are likely more protein-hungry and need more protein to produce the critical egg load necessary for oviposition. ABSTRACT: Olfaction plays a key role in the location of food and oviposition resources by tephritid fruit flies. Adult females, including oriental fruit fly, Bactrocera dorsalis, can sustain egg production throughout their lives provided they obtain sufficient protein. Thus, preferential attraction to food or oviposition sites (host fruit) will depend on a fly’s particular physiological state. In this study, laboratory bioassays were conducted with mature, mated B. dorsalis (provisioned protein and sugar ad libitum) to evaluate attraction to traps baited with torula yeast versus six host fruit sources (guava, guava juice, mango, orange, Surinam cherry, or white sapote). Females that preferred fruit laid a significant number of eggs around the trap entrance (average 405 eggs/fly), while almost no eggs were laid by females that preferred yeast (0.5 and 1.3 eggs/fly on two occasions). Similar results were observed in a bioassay using headspace extracts of guava juice and torula yeast, supporting olfactory-mediated responses. When individual females were allowed to oviposit in guava juice traps 0–24 h after a choice test, 45.8% of females that chose guava juice laid eggs (average 14.7 eggs/fly), compared with 27.5% that chose yeast (average 6.5 eggs/fly). Dissections indicated that females with a preference for guava juice had an egg load 2.4 times greater than females that preferred yeast. These results suggest there is an olfactory-based behavioral switch in preference from protein to host odors when female B. dorsalis are oviposition-ready. We discuss the implications of our findings for practical behavioral management and detection programs for B. dorsalis.