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Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves

Fluoroquinolones, such as danofloxacin, are used to control bovine respiratory disease complex in beef cattle; however, little is known about their effects on gut microbiota and resistome. The objectives were to evaluate the effect of subcutaneously administered danofloxacin on gut microbiota and re...

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Autores principales: Beyi, Ashenafi Feyisa, Brito-Goulart, Debora, Hawbecker, Tyler, Slagel, Clare, Ruddell, Brandon, Hassall, Alan, Dewell, Renee, Dewell, Grant, Sahin, Orhan, Zhang, Qijing, Plummer, Paul J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538188/
https://www.ncbi.nlm.nih.gov/pubmed/34683343
http://dx.doi.org/10.3390/microorganisms9102023
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author Beyi, Ashenafi Feyisa
Brito-Goulart, Debora
Hawbecker, Tyler
Slagel, Clare
Ruddell, Brandon
Hassall, Alan
Dewell, Renee
Dewell, Grant
Sahin, Orhan
Zhang, Qijing
Plummer, Paul J.
author_facet Beyi, Ashenafi Feyisa
Brito-Goulart, Debora
Hawbecker, Tyler
Slagel, Clare
Ruddell, Brandon
Hassall, Alan
Dewell, Renee
Dewell, Grant
Sahin, Orhan
Zhang, Qijing
Plummer, Paul J.
author_sort Beyi, Ashenafi Feyisa
collection PubMed
description Fluoroquinolones, such as danofloxacin, are used to control bovine respiratory disease complex in beef cattle; however, little is known about their effects on gut microbiota and resistome. The objectives were to evaluate the effect of subcutaneously administered danofloxacin on gut microbiota and resistome, and the composition of Campylobacter in calves. Twenty calves were injected with a single dose of danofloxacin, and ten calves were kept as a control. The effects of danofloxacin on microbiota and the resistome were assessed using 16S rRNA sequencing, quantitative real-time PCR, and metagenomic Hi-C ProxiMeta. Alpha and beta diversities were significantly different (p < 0.05) between pre-and post-treatment samples, and the compositions of several bacterial taxa shifted. The patterns of association between the compositions of Campylobacter and other genera were affected by danofloxacin. Antimicrobial resistance genes (ARGs) conferring resistance to five antibiotics were identified with their respective reservoirs. Following the treatment, some ARGs (e.g., ant9, tet40, tetW) increased in frequencies and host ranges, suggesting initiation of horizontal gene transfer, and new ARGs (aac6, ermF, tetL, tetX) were detected in the post-treatment samples. In conclusion, danofloxacin induced alterations of gut microbiota and selection and enrichment of resistance genes even against antibiotics that are unrelated to danofloxacin.
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spelling pubmed-85381882021-10-24 Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves Beyi, Ashenafi Feyisa Brito-Goulart, Debora Hawbecker, Tyler Slagel, Clare Ruddell, Brandon Hassall, Alan Dewell, Renee Dewell, Grant Sahin, Orhan Zhang, Qijing Plummer, Paul J. Microorganisms Article Fluoroquinolones, such as danofloxacin, are used to control bovine respiratory disease complex in beef cattle; however, little is known about their effects on gut microbiota and resistome. The objectives were to evaluate the effect of subcutaneously administered danofloxacin on gut microbiota and resistome, and the composition of Campylobacter in calves. Twenty calves were injected with a single dose of danofloxacin, and ten calves were kept as a control. The effects of danofloxacin on microbiota and the resistome were assessed using 16S rRNA sequencing, quantitative real-time PCR, and metagenomic Hi-C ProxiMeta. Alpha and beta diversities were significantly different (p < 0.05) between pre-and post-treatment samples, and the compositions of several bacterial taxa shifted. The patterns of association between the compositions of Campylobacter and other genera were affected by danofloxacin. Antimicrobial resistance genes (ARGs) conferring resistance to five antibiotics were identified with their respective reservoirs. Following the treatment, some ARGs (e.g., ant9, tet40, tetW) increased in frequencies and host ranges, suggesting initiation of horizontal gene transfer, and new ARGs (aac6, ermF, tetL, tetX) were detected in the post-treatment samples. In conclusion, danofloxacin induced alterations of gut microbiota and selection and enrichment of resistance genes even against antibiotics that are unrelated to danofloxacin. MDPI 2021-09-24 /pmc/articles/PMC8538188/ /pubmed/34683343 http://dx.doi.org/10.3390/microorganisms9102023 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Beyi, Ashenafi Feyisa
Brito-Goulart, Debora
Hawbecker, Tyler
Slagel, Clare
Ruddell, Brandon
Hassall, Alan
Dewell, Renee
Dewell, Grant
Sahin, Orhan
Zhang, Qijing
Plummer, Paul J.
Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves
title Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves
title_full Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves
title_fullStr Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves
title_full_unstemmed Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves
title_short Danofloxacin Treatment Alters the Diversity and Resistome Profile of Gut Microbiota in Calves
title_sort danofloxacin treatment alters the diversity and resistome profile of gut microbiota in calves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538188/
https://www.ncbi.nlm.nih.gov/pubmed/34683343
http://dx.doi.org/10.3390/microorganisms9102023
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