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Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways

Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune...

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Autores principales: Xu, Yulin, Ye, Mengxue, Zhang, Youwen, Sun, Shaohua, Luo, Jia, Jiang, Sen, Zhang, Jiajia, Liu, Xueliang, Shao, Qi, Cao, Qi, Zheng, Wanglong, Meurens, François, Chen, Nanhua, Zhu, Jianzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538207/
https://www.ncbi.nlm.nih.gov/pubmed/34696285
http://dx.doi.org/10.3390/vaccines9101176
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author Xu, Yulin
Ye, Mengxue
Zhang, Youwen
Sun, Shaohua
Luo, Jia
Jiang, Sen
Zhang, Jiajia
Liu, Xueliang
Shao, Qi
Cao, Qi
Zheng, Wanglong
Meurens, François
Chen, Nanhua
Zhu, Jianzhong
author_facet Xu, Yulin
Ye, Mengxue
Zhang, Youwen
Sun, Shaohua
Luo, Jia
Jiang, Sen
Zhang, Jiajia
Liu, Xueliang
Shao, Qi
Cao, Qi
Zheng, Wanglong
Meurens, François
Chen, Nanhua
Zhu, Jianzhong
author_sort Xu, Yulin
collection PubMed
description Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies.
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spelling pubmed-85382072021-10-24 Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways Xu, Yulin Ye, Mengxue Zhang, Youwen Sun, Shaohua Luo, Jia Jiang, Sen Zhang, Jiajia Liu, Xueliang Shao, Qi Cao, Qi Zheng, Wanglong Meurens, François Chen, Nanhua Zhu, Jianzhong Vaccines (Basel) Article Porcine reproductive and respiratory syndrome virus (PRRSV) causes PRRS and is known to effectively suppress host innate immunity. The current strategies for controlling PRRSV are limited and complete understanding of anti-PRRSV innate immunity is needed. Here, we utilized nine porcine innate immune signaling adaptors which represent all currently known innate immune receptor signaling pathways for screening of anti-PRRSV activity. The analysis of PRRSV N gene transcription and protein expression both suggested that the multiple ectopic adaptors exhibited varying degrees of anti-PRRSV activities, with TRIF and MAVS most effective. To better quantify the PRRSV replication, the GFP signal of PRRSV from reverse genetics were measured by flow cytometry and similarly varying anti-PRRSV activities by different signaling adaptors were observed. Based on the screening data, and considering the importance of viral nucleic acid in innate immune response, endogenous TRIF, MAVS and STING were selected for further examination of anti-PRRSV activity. Agonist stimulation assay showed that MAVS and STING signaling possessed significant anti-PRRSV activities, whereas siRNA knockdown assay showed that TRIF, MAVS and STING are all involved in anti-PRRSV activity, with TLR3-TRIF displaying discrepancy in anti-PRRSV infection. Nevertheless, our work suggests that multiple pattern recognition receptor (PRR) signaling pathways are involved in anti-PRRSV innate immunity, which may have implications for the development of future antiviral strategies. MDPI 2021-10-14 /pmc/articles/PMC8538207/ /pubmed/34696285 http://dx.doi.org/10.3390/vaccines9101176 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xu, Yulin
Ye, Mengxue
Zhang, Youwen
Sun, Shaohua
Luo, Jia
Jiang, Sen
Zhang, Jiajia
Liu, Xueliang
Shao, Qi
Cao, Qi
Zheng, Wanglong
Meurens, François
Chen, Nanhua
Zhu, Jianzhong
Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
title Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
title_full Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
title_fullStr Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
title_full_unstemmed Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
title_short Screening of Porcine Innate Immune Adaptor Signaling Revealed Several Anti-PRRSV Signaling Pathways
title_sort screening of porcine innate immune adaptor signaling revealed several anti-prrsv signaling pathways
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538207/
https://www.ncbi.nlm.nih.gov/pubmed/34696285
http://dx.doi.org/10.3390/vaccines9101176
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