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IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women

Our study aimed to evaluate the distribution of genotypes and allele frequencies of IL-6 597A/G (rs1800797) and 174G/C (rs1800795) polymorphisms in HPV infected and uninfected healthy women and cervical cancer patients. A PCR based Multiplex HPV genotyping test kit was used for in vitro detection an...

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Autores principales: Vitkauskaite, Agne, Celiesiute, Joana, Juseviciute, Vijoleta, Jariene, Kristina, Skrodeniene, Erika, Samuolyte, Gabriele, Nadisauskiene, Ruta Jolanta, Vaitkiene, Daiva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538235/
https://www.ncbi.nlm.nih.gov/pubmed/34684062
http://dx.doi.org/10.3390/medicina57101025
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author Vitkauskaite, Agne
Celiesiute, Joana
Juseviciute, Vijoleta
Jariene, Kristina
Skrodeniene, Erika
Samuolyte, Gabriele
Nadisauskiene, Ruta Jolanta
Vaitkiene, Daiva
author_facet Vitkauskaite, Agne
Celiesiute, Joana
Juseviciute, Vijoleta
Jariene, Kristina
Skrodeniene, Erika
Samuolyte, Gabriele
Nadisauskiene, Ruta Jolanta
Vaitkiene, Daiva
author_sort Vitkauskaite, Agne
collection PubMed
description Our study aimed to evaluate the distribution of genotypes and allele frequencies of IL-6 597A/G (rs1800797) and 174G/C (rs1800795) polymorphisms in HPV infected and uninfected healthy women and cervical cancer patients. A PCR based Multiplex HPV genotyping test kit was used for in vitro detection and differentiation of high risk HPV genotypes. Genotyping of two polymorphisms, IL-6 597A/G (rs1800797) and 174G/C (rs1800795), was performed using the KASP genotyping assay kit. Cervical cancer patients were more likely to be HPV positive than control patients. Allele C of IL-6 rs1800795 was associated with a higher risk of cervical cancer by 2.26-fold and genotype CC by 5.37-fold. Genotype CC of IL-6 rs1800795 was more frequent in the HPV positive group compared with the HPV negative group (p = 0.002). Allele G of IL-6 rs1800797 was more frequently found in women with HPV16/HPV18 compared to other HPV types (p = 0.045). Women with AA genotypes of IL-6 rs1800797 were less frequently infected with HPV16/HPV18 compared to other HPV types (p = 0.045). The major finding of the study is the significant association of C allele and CC genotype of IL-6 1800795 gene with cervical cancer in the Lithuanian population. Genotype CC of IL-6 rs1800795 has a significant association with HPV infection as well.
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spelling pubmed-85382352021-10-24 IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women Vitkauskaite, Agne Celiesiute, Joana Juseviciute, Vijoleta Jariene, Kristina Skrodeniene, Erika Samuolyte, Gabriele Nadisauskiene, Ruta Jolanta Vaitkiene, Daiva Medicina (Kaunas) Article Our study aimed to evaluate the distribution of genotypes and allele frequencies of IL-6 597A/G (rs1800797) and 174G/C (rs1800795) polymorphisms in HPV infected and uninfected healthy women and cervical cancer patients. A PCR based Multiplex HPV genotyping test kit was used for in vitro detection and differentiation of high risk HPV genotypes. Genotyping of two polymorphisms, IL-6 597A/G (rs1800797) and 174G/C (rs1800795), was performed using the KASP genotyping assay kit. Cervical cancer patients were more likely to be HPV positive than control patients. Allele C of IL-6 rs1800795 was associated with a higher risk of cervical cancer by 2.26-fold and genotype CC by 5.37-fold. Genotype CC of IL-6 rs1800795 was more frequent in the HPV positive group compared with the HPV negative group (p = 0.002). Allele G of IL-6 rs1800797 was more frequently found in women with HPV16/HPV18 compared to other HPV types (p = 0.045). Women with AA genotypes of IL-6 rs1800797 were less frequently infected with HPV16/HPV18 compared to other HPV types (p = 0.045). The major finding of the study is the significant association of C allele and CC genotype of IL-6 1800795 gene with cervical cancer in the Lithuanian population. Genotype CC of IL-6 rs1800795 has a significant association with HPV infection as well. MDPI 2021-09-26 /pmc/articles/PMC8538235/ /pubmed/34684062 http://dx.doi.org/10.3390/medicina57101025 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vitkauskaite, Agne
Celiesiute, Joana
Juseviciute, Vijoleta
Jariene, Kristina
Skrodeniene, Erika
Samuolyte, Gabriele
Nadisauskiene, Ruta Jolanta
Vaitkiene, Daiva
IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women
title IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women
title_full IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women
title_fullStr IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women
title_full_unstemmed IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women
title_short IL-6 597A/G (rs1800797) and 174G/C (rs1800795) Gene Polymorphisms in the Development of Cervical Cancer in Lithuanian Women
title_sort il-6 597a/g (rs1800797) and 174g/c (rs1800795) gene polymorphisms in the development of cervical cancer in lithuanian women
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538235/
https://www.ncbi.nlm.nih.gov/pubmed/34684062
http://dx.doi.org/10.3390/medicina57101025
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