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Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)

The synthesis of the benzylisoquinoline alkaloids, sanguinarine and berberine, was monitored in Argemone mexicana L. (Papaveracea) throughout the early stages of its hypocotyl and seedling development. Sanguinarine was detected in the cotyledons right after hypocotyl emergence, and it increased cont...

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Autores principales: Xool-Tamayo, Jorge, Tamayo-Ordoñez, Yahaira, Monforte-González, Miriam, Muñoz-Sánchez, José Armando, Vázquez-Flota, Felipe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538276/
https://www.ncbi.nlm.nih.gov/pubmed/34686035
http://dx.doi.org/10.3390/plants10102226
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author Xool-Tamayo, Jorge
Tamayo-Ordoñez, Yahaira
Monforte-González, Miriam
Muñoz-Sánchez, José Armando
Vázquez-Flota, Felipe
author_facet Xool-Tamayo, Jorge
Tamayo-Ordoñez, Yahaira
Monforte-González, Miriam
Muñoz-Sánchez, José Armando
Vázquez-Flota, Felipe
author_sort Xool-Tamayo, Jorge
collection PubMed
description The synthesis of the benzylisoquinoline alkaloids, sanguinarine and berberine, was monitored in Argemone mexicana L. (Papaveracea) throughout the early stages of its hypocotyl and seedling development. Sanguinarine was detected in the cotyledons right after hypocotyl emergence, and it increased continuously until the apical hook unbent, prior to the cotyledonary leaves unfolding, when it abruptly fell. In the cotyledonary leaves, it also remained at low levels. Throughout development, berberine accumulation required the formation of cotyledonary leaves, whereas it was quickly detected in the hypocotyl from the time it emerged. Interestingly, the alkaloids detected in the cotyledons could have been imported from hypocotyls, because no transcriptional activity was detected in there. However, after turning into cotyledonary leaves, important levels of gene expression were noted. Taken together, these results suggest that the patterns of alkaloid tissue distribution are established from very early development, and might require transport systems.
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spelling pubmed-85382762021-10-24 Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae) Xool-Tamayo, Jorge Tamayo-Ordoñez, Yahaira Monforte-González, Miriam Muñoz-Sánchez, José Armando Vázquez-Flota, Felipe Plants (Basel) Communication The synthesis of the benzylisoquinoline alkaloids, sanguinarine and berberine, was monitored in Argemone mexicana L. (Papaveracea) throughout the early stages of its hypocotyl and seedling development. Sanguinarine was detected in the cotyledons right after hypocotyl emergence, and it increased continuously until the apical hook unbent, prior to the cotyledonary leaves unfolding, when it abruptly fell. In the cotyledonary leaves, it also remained at low levels. Throughout development, berberine accumulation required the formation of cotyledonary leaves, whereas it was quickly detected in the hypocotyl from the time it emerged. Interestingly, the alkaloids detected in the cotyledons could have been imported from hypocotyls, because no transcriptional activity was detected in there. However, after turning into cotyledonary leaves, important levels of gene expression were noted. Taken together, these results suggest that the patterns of alkaloid tissue distribution are established from very early development, and might require transport systems. MDPI 2021-10-19 /pmc/articles/PMC8538276/ /pubmed/34686035 http://dx.doi.org/10.3390/plants10102226 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Xool-Tamayo, Jorge
Tamayo-Ordoñez, Yahaira
Monforte-González, Miriam
Muñoz-Sánchez, José Armando
Vázquez-Flota, Felipe
Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)
title Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)
title_full Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)
title_fullStr Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)
title_full_unstemmed Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)
title_short Alkaloid Biosynthesis in the Early Stages of the Germination of Argemone mexicana L. (Papaveraceae)
title_sort alkaloid biosynthesis in the early stages of the germination of argemone mexicana l. (papaveraceae)
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538276/
https://www.ncbi.nlm.nih.gov/pubmed/34686035
http://dx.doi.org/10.3390/plants10102226
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