Cargando…

Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging

Cyanotoxins can be found in water and air during cyanobacterial harmful algal blooms (cHABs) in lakes and rivers. Therefore, it is very important to monitor their potential uptake by animals and humans as well as their health effects and distribution in affected organs. Herein, the distribution of h...

Descripción completa

Detalles Bibliográficos
Autores principales: Kucheriavaia, Daria, Veličković, Dušan, Peraino, Nicholas, Lad, Apurva, Kennedy, David J., Haller, Steven T., Westrick, Judy A., Isailovic, Dragan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538440/
https://www.ncbi.nlm.nih.gov/pubmed/34679004
http://dx.doi.org/10.3390/toxins13100709
_version_ 1784588505852149760
author Kucheriavaia, Daria
Veličković, Dušan
Peraino, Nicholas
Lad, Apurva
Kennedy, David J.
Haller, Steven T.
Westrick, Judy A.
Isailovic, Dragan
author_facet Kucheriavaia, Daria
Veličković, Dušan
Peraino, Nicholas
Lad, Apurva
Kennedy, David J.
Haller, Steven T.
Westrick, Judy A.
Isailovic, Dragan
author_sort Kucheriavaia, Daria
collection PubMed
description Cyanotoxins can be found in water and air during cyanobacterial harmful algal blooms (cHABs) in lakes and rivers. Therefore, it is very important to monitor their potential uptake by animals and humans as well as their health effects and distribution in affected organs. Herein, the distribution of hepatotoxic peptide microcystin-LR (MC-LR) is investigated in liver tissues of mice gavaged with this most common MC congener. Preliminary matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging experiments performed using a non-automated MALDI matrix deposition device and a MALDI-time-of-flight (TOF) mass spectrometer yielded ambiguous results in terms of MC-LR distribution in liver samples obtained from MC-LR-gavaged mice. The tissue preparation for MALDI-MS imaging was improved by using an automated sprayer for matrix deposition, and liver sections were imaged using an Nd:YAG MALDI laser coupled to a 15 Tesla Fourier-transform ion cyclotron resonance (FT-ICR)-mass spectrometer. MALDI-FT-ICR-MS imaging provided unambiguous detection of protonated MC-LR (calculated m/z 995.5560, z = +1) and the sodium adduct of MC-LR (m/z 1017.5380, z = +1) in liver sections from gavaged mice with great mass accuracy and ultra-high mass resolution. Since both covalently bound and free MC-LR can be found in liver of mice exposed to this toxin, the present results indicate that the distribution of free microcystins in tissue sections from affected organs, such as liver, can be monitored with high-resolution MALDI-MS imaging.
format Online
Article
Text
id pubmed-8538440
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-85384402021-10-24 Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging Kucheriavaia, Daria Veličković, Dušan Peraino, Nicholas Lad, Apurva Kennedy, David J. Haller, Steven T. Westrick, Judy A. Isailovic, Dragan Toxins (Basel) Article Cyanotoxins can be found in water and air during cyanobacterial harmful algal blooms (cHABs) in lakes and rivers. Therefore, it is very important to monitor their potential uptake by animals and humans as well as their health effects and distribution in affected organs. Herein, the distribution of hepatotoxic peptide microcystin-LR (MC-LR) is investigated in liver tissues of mice gavaged with this most common MC congener. Preliminary matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging experiments performed using a non-automated MALDI matrix deposition device and a MALDI-time-of-flight (TOF) mass spectrometer yielded ambiguous results in terms of MC-LR distribution in liver samples obtained from MC-LR-gavaged mice. The tissue preparation for MALDI-MS imaging was improved by using an automated sprayer for matrix deposition, and liver sections were imaged using an Nd:YAG MALDI laser coupled to a 15 Tesla Fourier-transform ion cyclotron resonance (FT-ICR)-mass spectrometer. MALDI-FT-ICR-MS imaging provided unambiguous detection of protonated MC-LR (calculated m/z 995.5560, z = +1) and the sodium adduct of MC-LR (m/z 1017.5380, z = +1) in liver sections from gavaged mice with great mass accuracy and ultra-high mass resolution. Since both covalently bound and free MC-LR can be found in liver of mice exposed to this toxin, the present results indicate that the distribution of free microcystins in tissue sections from affected organs, such as liver, can be monitored with high-resolution MALDI-MS imaging. MDPI 2021-10-08 /pmc/articles/PMC8538440/ /pubmed/34679004 http://dx.doi.org/10.3390/toxins13100709 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kucheriavaia, Daria
Veličković, Dušan
Peraino, Nicholas
Lad, Apurva
Kennedy, David J.
Haller, Steven T.
Westrick, Judy A.
Isailovic, Dragan
Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging
title Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging
title_full Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging
title_fullStr Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging
title_full_unstemmed Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging
title_short Toward Revealing Microcystin Distribution in Mouse Liver Tissue Using MALDI-MS Imaging
title_sort toward revealing microcystin distribution in mouse liver tissue using maldi-ms imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538440/
https://www.ncbi.nlm.nih.gov/pubmed/34679004
http://dx.doi.org/10.3390/toxins13100709
work_keys_str_mv AT kucheriavaiadaria towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging
AT velickovicdusan towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging
AT perainonicholas towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging
AT ladapurva towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging
AT kennedydavidj towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging
AT hallerstevent towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging
AT westrickjudya towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging
AT isailovicdragan towardrevealingmicrocystindistributioninmouselivertissueusingmaldimsimaging