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The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses

Bcl-2-associated athanogene (BAG), a group of proteins evolutionarily conserved and functioned as co-chaperones in plants and animals, is involved in various cell activities and diverse physiological processes. However, the biological functions of this gene family in rice are largely unknown. In thi...

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Autores principales: Zhou, Hailian, Li, Jiaying, Liu, Xueyuan, Wei, Xiaoshuang, He, Ziwei, Hu, Lihua, Wang, Jibin, Duan, Mingzheng, Xie, Guosheng, Wang, Jihong, Wang, Lingqiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538510/
https://www.ncbi.nlm.nih.gov/pubmed/34685978
http://dx.doi.org/10.3390/plants10102169
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author Zhou, Hailian
Li, Jiaying
Liu, Xueyuan
Wei, Xiaoshuang
He, Ziwei
Hu, Lihua
Wang, Jibin
Duan, Mingzheng
Xie, Guosheng
Wang, Jihong
Wang, Lingqiang
author_facet Zhou, Hailian
Li, Jiaying
Liu, Xueyuan
Wei, Xiaoshuang
He, Ziwei
Hu, Lihua
Wang, Jibin
Duan, Mingzheng
Xie, Guosheng
Wang, Jihong
Wang, Lingqiang
author_sort Zhou, Hailian
collection PubMed
description Bcl-2-associated athanogene (BAG), a group of proteins evolutionarily conserved and functioned as co-chaperones in plants and animals, is involved in various cell activities and diverse physiological processes. However, the biological functions of this gene family in rice are largely unknown. In this study, we identified a total of six BAG members in rice. These genes were classified into two groups, OsBAG1, -2, -3, and -4 are in group I with a conserved ubiquitin-like structure and OsBAG5 and -6 are in group Ⅱ with a calmodulin-binding domain, in addition to a common BAG domain. The BAG genes exhibited diverse expression patterns, with OsBAG4 showing the highest expression level, followed by OsBAG1 and OsBAG3, and OsBAG6 preferentially expressed in the panicle, endosperm, and calli. The co-expression analysis and the hierarchical cluster analysis indicated that the OsBAG1 and OsBAG3 were co-expressed with primary cell wall-biosynthesizing genes, OsBAG4 was co-expressed with phytohormone and transcriptional factors, and OsBAG6 was co-expressed with disease and shock-associated genes. β-glucuronidase (GUS) staining further indicated that OsBAG3 is mainly involved in primary young tissues under both primary and secondary growth. In addition, the expression of the BAG genes under brown planthopper (BPH) feeding, N, P, and K deficiency, heat, drought and plant hormones treatments was investigated. Our results clearly showed that OsBAGs are multifunctional molecules as inferred by their protein structures, subcellular localizations, and expression profiles. BAGs in group I are mainly involved in plant development, whereas BAGs in group II are reactive in gene regulations and stress responses. Our results provide a solid basis for the further elucidation of the biological functions of plant BAG genes.
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spelling pubmed-85385102021-10-24 The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses Zhou, Hailian Li, Jiaying Liu, Xueyuan Wei, Xiaoshuang He, Ziwei Hu, Lihua Wang, Jibin Duan, Mingzheng Xie, Guosheng Wang, Jihong Wang, Lingqiang Plants (Basel) Article Bcl-2-associated athanogene (BAG), a group of proteins evolutionarily conserved and functioned as co-chaperones in plants and animals, is involved in various cell activities and diverse physiological processes. However, the biological functions of this gene family in rice are largely unknown. In this study, we identified a total of six BAG members in rice. These genes were classified into two groups, OsBAG1, -2, -3, and -4 are in group I with a conserved ubiquitin-like structure and OsBAG5 and -6 are in group Ⅱ with a calmodulin-binding domain, in addition to a common BAG domain. The BAG genes exhibited diverse expression patterns, with OsBAG4 showing the highest expression level, followed by OsBAG1 and OsBAG3, and OsBAG6 preferentially expressed in the panicle, endosperm, and calli. The co-expression analysis and the hierarchical cluster analysis indicated that the OsBAG1 and OsBAG3 were co-expressed with primary cell wall-biosynthesizing genes, OsBAG4 was co-expressed with phytohormone and transcriptional factors, and OsBAG6 was co-expressed with disease and shock-associated genes. β-glucuronidase (GUS) staining further indicated that OsBAG3 is mainly involved in primary young tissues under both primary and secondary growth. In addition, the expression of the BAG genes under brown planthopper (BPH) feeding, N, P, and K deficiency, heat, drought and plant hormones treatments was investigated. Our results clearly showed that OsBAGs are multifunctional molecules as inferred by their protein structures, subcellular localizations, and expression profiles. BAGs in group I are mainly involved in plant development, whereas BAGs in group II are reactive in gene regulations and stress responses. Our results provide a solid basis for the further elucidation of the biological functions of plant BAG genes. MDPI 2021-10-13 /pmc/articles/PMC8538510/ /pubmed/34685978 http://dx.doi.org/10.3390/plants10102169 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhou, Hailian
Li, Jiaying
Liu, Xueyuan
Wei, Xiaoshuang
He, Ziwei
Hu, Lihua
Wang, Jibin
Duan, Mingzheng
Xie, Guosheng
Wang, Jihong
Wang, Lingqiang
The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses
title The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses
title_full The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses
title_fullStr The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses
title_full_unstemmed The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses
title_short The Divergent Roles of the Rice bcl-2 Associated Athanogene (BAG) Genes in Plant Development and Environmental Responses
title_sort divergent roles of the rice bcl-2 associated athanogene (bag) genes in plant development and environmental responses
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538510/
https://www.ncbi.nlm.nih.gov/pubmed/34685978
http://dx.doi.org/10.3390/plants10102169
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