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Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam

Avian Metapneumovirus (aMPV) is a causative agent of respiratory disease complex in turkeys and chickens that has recently been detected in Vietnam. Due to its novelty, this study was conducted to elucidate the distribution of aMPV in several provinces in northern Vietnam. By the application of Enzy...

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Autores principales: Nguyen, Van-Giap, Chung, Hee-Chun, Do, Hai-Quynh, Nguyen, Thanh-Trung, Cao, Thi-Bich-Phuong, Truong, Ha-Thai, Mai, Thi-Ngan, Le, Thi-Trinh, Nguyen, Thi-Hoa, Le, Thi-Luyen, Huynh, Thi-My-Le
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538526/
https://www.ncbi.nlm.nih.gov/pubmed/34679036
http://dx.doi.org/10.3390/vetsci8100206
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author Nguyen, Van-Giap
Chung, Hee-Chun
Do, Hai-Quynh
Nguyen, Thanh-Trung
Cao, Thi-Bich-Phuong
Truong, Ha-Thai
Mai, Thi-Ngan
Le, Thi-Trinh
Nguyen, Thi-Hoa
Le, Thi-Luyen
Huynh, Thi-My-Le
author_facet Nguyen, Van-Giap
Chung, Hee-Chun
Do, Hai-Quynh
Nguyen, Thanh-Trung
Cao, Thi-Bich-Phuong
Truong, Ha-Thai
Mai, Thi-Ngan
Le, Thi-Trinh
Nguyen, Thi-Hoa
Le, Thi-Luyen
Huynh, Thi-My-Le
author_sort Nguyen, Van-Giap
collection PubMed
description Avian Metapneumovirus (aMPV) is a causative agent of respiratory disease complex in turkeys and chickens that has recently been detected in Vietnam. Due to its novelty, this study was conducted to elucidate the distribution of aMPV in several provinces in northern Vietnam. By the application of Enzyme-Linked Immunosorbent Assay (ELISA) and nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR), this study demonstrated the circulation of aMPV in 12 out of 14 cities/provinces with positive rates of 37.6% and 17.2%, respectively. All nested RT-PCR positive samples were aMPV subgroup B. By pairing the detection results with age groups, it was observed that aMPV infections occurred in chickens of all ages. Additionally, by genetic characterization, aMPV strains were demonstrated to not be attenuated vaccine viruses and to belong to at least two genetic clades. Overall, the obtained results provided insights into the prevalence of aMPV and indicated a greater complexity of respiratory diseases in chickens in Vietnam.
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spelling pubmed-85385262021-10-24 Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam Nguyen, Van-Giap Chung, Hee-Chun Do, Hai-Quynh Nguyen, Thanh-Trung Cao, Thi-Bich-Phuong Truong, Ha-Thai Mai, Thi-Ngan Le, Thi-Trinh Nguyen, Thi-Hoa Le, Thi-Luyen Huynh, Thi-My-Le Vet Sci Article Avian Metapneumovirus (aMPV) is a causative agent of respiratory disease complex in turkeys and chickens that has recently been detected in Vietnam. Due to its novelty, this study was conducted to elucidate the distribution of aMPV in several provinces in northern Vietnam. By the application of Enzyme-Linked Immunosorbent Assay (ELISA) and nested Reverse Transcription-Polymerase Chain Reaction (RT-PCR), this study demonstrated the circulation of aMPV in 12 out of 14 cities/provinces with positive rates of 37.6% and 17.2%, respectively. All nested RT-PCR positive samples were aMPV subgroup B. By pairing the detection results with age groups, it was observed that aMPV infections occurred in chickens of all ages. Additionally, by genetic characterization, aMPV strains were demonstrated to not be attenuated vaccine viruses and to belong to at least two genetic clades. Overall, the obtained results provided insights into the prevalence of aMPV and indicated a greater complexity of respiratory diseases in chickens in Vietnam. MDPI 2021-09-24 /pmc/articles/PMC8538526/ /pubmed/34679036 http://dx.doi.org/10.3390/vetsci8100206 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nguyen, Van-Giap
Chung, Hee-Chun
Do, Hai-Quynh
Nguyen, Thanh-Trung
Cao, Thi-Bich-Phuong
Truong, Ha-Thai
Mai, Thi-Ngan
Le, Thi-Trinh
Nguyen, Thi-Hoa
Le, Thi-Luyen
Huynh, Thi-My-Le
Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam
title Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam
title_full Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam
title_fullStr Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam
title_full_unstemmed Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam
title_short Serological and Molecular Characterization of Avian Metapneumovirus in Chickens in Northern Vietnam
title_sort serological and molecular characterization of avian metapneumovirus in chickens in northern vietnam
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538526/
https://www.ncbi.nlm.nih.gov/pubmed/34679036
http://dx.doi.org/10.3390/vetsci8100206
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