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Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season

Influenza B viruses (IBVs) are causing an increasing burden of morbidity and mortality, yet the prevalence of culture-adapted mutations in human seasonal IBVs are unclear. We collected 368 clinical samples from patients with influenza-like illness in Missouri during the 2019–2020 influenza season an...

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Autores principales: Tang, Cynthia Y., Segovia, Karen, McElroy, Jane A., Li, Tao, Guan, Minhui, Zhang, Xiaojian, Misra, Shamita, Hang, Jun, Wan, Xiu-Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538563/
https://www.ncbi.nlm.nih.gov/pubmed/34696325
http://dx.doi.org/10.3390/v13101896
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author Tang, Cynthia Y.
Segovia, Karen
McElroy, Jane A.
Li, Tao
Guan, Minhui
Zhang, Xiaojian
Misra, Shamita
Hang, Jun
Wan, Xiu-Feng
author_facet Tang, Cynthia Y.
Segovia, Karen
McElroy, Jane A.
Li, Tao
Guan, Minhui
Zhang, Xiaojian
Misra, Shamita
Hang, Jun
Wan, Xiu-Feng
author_sort Tang, Cynthia Y.
collection PubMed
description Influenza B viruses (IBVs) are causing an increasing burden of morbidity and mortality, yet the prevalence of culture-adapted mutations in human seasonal IBVs are unclear. We collected 368 clinical samples from patients with influenza-like illness in Missouri during the 2019–2020 influenza season and recovered 146 influenza isolates including 38 IBV isolates. Of MDCK-CCL34, MDCK-Siat1, and humanized MDCK (hCK), hCK showed the highest virus recovery efficiency. All Missourian IBVs belonged to the Victoria V1A.3 lineage, all of which contained a three-amino acid deletion on the HA protein and were antigenically distant from the Victoria lineage IBV vaccine strain used during that season. By comparing genomic sequences of these IBVs in 31 paired samples, eight cell-adapted nonsynonymous mutations were identified, with the majority in the RNA polymerase. Analyses of IBV clinical sample–isolate pairs from public databases further showed that cell- and egg-adapted mutations occurred more widely in viral proteins, including the receptor and antibody binding sites on HA. Our study suggests that hCK is an effective platform for IBV isolation and that culture-adapted mutations may occur during IBV isolation. As culture-adapted mutations may affect subsequent virus studies and vaccine development, the knowledge from this study may help optimize strategies for influenza surveillance, vaccine strain selection, and vaccine development.
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spelling pubmed-85385632021-10-24 Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season Tang, Cynthia Y. Segovia, Karen McElroy, Jane A. Li, Tao Guan, Minhui Zhang, Xiaojian Misra, Shamita Hang, Jun Wan, Xiu-Feng Viruses Article Influenza B viruses (IBVs) are causing an increasing burden of morbidity and mortality, yet the prevalence of culture-adapted mutations in human seasonal IBVs are unclear. We collected 368 clinical samples from patients with influenza-like illness in Missouri during the 2019–2020 influenza season and recovered 146 influenza isolates including 38 IBV isolates. Of MDCK-CCL34, MDCK-Siat1, and humanized MDCK (hCK), hCK showed the highest virus recovery efficiency. All Missourian IBVs belonged to the Victoria V1A.3 lineage, all of which contained a three-amino acid deletion on the HA protein and were antigenically distant from the Victoria lineage IBV vaccine strain used during that season. By comparing genomic sequences of these IBVs in 31 paired samples, eight cell-adapted nonsynonymous mutations were identified, with the majority in the RNA polymerase. Analyses of IBV clinical sample–isolate pairs from public databases further showed that cell- and egg-adapted mutations occurred more widely in viral proteins, including the receptor and antibody binding sites on HA. Our study suggests that hCK is an effective platform for IBV isolation and that culture-adapted mutations may occur during IBV isolation. As culture-adapted mutations may affect subsequent virus studies and vaccine development, the knowledge from this study may help optimize strategies for influenza surveillance, vaccine strain selection, and vaccine development. MDPI 2021-09-22 /pmc/articles/PMC8538563/ /pubmed/34696325 http://dx.doi.org/10.3390/v13101896 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tang, Cynthia Y.
Segovia, Karen
McElroy, Jane A.
Li, Tao
Guan, Minhui
Zhang, Xiaojian
Misra, Shamita
Hang, Jun
Wan, Xiu-Feng
Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season
title Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season
title_full Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season
title_fullStr Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season
title_full_unstemmed Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season
title_short Cell-Adapted Mutations and Antigenic Diversity of Influenza B Viruses in Missouri, 2019–2020 Season
title_sort cell-adapted mutations and antigenic diversity of influenza b viruses in missouri, 2019–2020 season
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538563/
https://www.ncbi.nlm.nih.gov/pubmed/34696325
http://dx.doi.org/10.3390/v13101896
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