Different Host Plants Distinctly Influence the Feeding Ability of the Brown Citrus Aphid Toxoptera citricida

SIMPLE SUMMARY: The brown citrus aphid, Toxoptera citricida, is an important pest of citrus and causes serious damage in the main production areas. Host plant resistance is an environmentally friendly method to manage aphid infestations and is becoming increasingly important as aphids develop greater resistance to insecticides. The aim of this study was to assess T. citricida resistance on seven widespread and common hosts using electropenetrography combined with a population development test. We showed that the feeding parameters of the brown citrus aphid differed significantly depending on the host plants. Toxoptera citricida spent more time in the pathway stage and less time in the phloem stage on Eureka, while the opposite was observed on Yuzu and Rough Lemon. Measurements of population development of aphids showed that on the Eureka, aphids developed more slowly. Our data suggest that different host plants distinctly influence the ability of T. citricida to feed. The brown citrus aphid did not prefer to feed on Eureka Lemon compared to the other six host plants. ABSTRACT: Piercing–sucking insects are important crop pests, and an understanding of their feeding behavior and population development plays a crucial role in studying insect population dynamics and crop resistance. In our study, we examined the probing behavior of the brown citrus aphid, Toxoptera citricida, using electropenetrography and assessed its population development after 8 days on seven host plants: Yuzu, Citrus junos Sieb. ex Tanaka; Rough Lemon, C. jambhiri Lush.; ‘Luofu’ kumquat, Fortunella margarita Swingle; ‘Olinda’ valencia orange, C. sinensis (L.) Osbeck; ‘Yanxiwanlu’ Ponkan, C. reticulata Blanco; ‘Rohde Red’ valencia orange, C. sinensis; and ‘Eureka’ lemon, C. limon (L.) Osbeck. The results demonstrated that probing by the brown citrus aphid differed significantly according to the target hosts. Toxoptera citricida produced significantly more pathway activities on Eureka than on Rough Lemon and Yuzu. Toxoptera citricida spent more time from the first probe to first salivation into phloem sieve elements on Eureka compared to Yuzu. In addition, the total duration of ingestion from sieve cells of each aphid in the phloem-feeding phase was shortest on Eureka, and this was significantly shorter than that on Yuzu, Rough Lemon, Luofu, and Olinda. The population number of T. citricida on Eureka after 8 days was significantly lower than that on the other hosts. Overall, Eureka was found to have obvious resistance to T. citricida, whereas Yuzu and Rough Lemon were susceptible host plants. These results provide a theoretical basis for exploring aphid-resistant fruit tree resources using resistant varieties.