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Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia

Malassezia are common components of human skin, and as the dominant human skin eukaryotic microbe, they take part in complex microbe–host interactions. Other phylogenetically related fungi (including within Ustilagomycotina) communicate with their plant host through bioactive oxygenated polyunsatura...

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Autores principales: Ambaw, Yohannes Abere, Pagac, Martin P., Irudayaswamy, Antony S., Raida, Manfred, Bendt, Anne K., Torta, Federico T., Wenk, Markus R., Dawson, Thomas L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538739/
https://www.ncbi.nlm.nih.gov/pubmed/34677414
http://dx.doi.org/10.3390/metabo11100700
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author Ambaw, Yohannes Abere
Pagac, Martin P.
Irudayaswamy, Antony S.
Raida, Manfred
Bendt, Anne K.
Torta, Federico T.
Wenk, Markus R.
Dawson, Thomas L.
author_facet Ambaw, Yohannes Abere
Pagac, Martin P.
Irudayaswamy, Antony S.
Raida, Manfred
Bendt, Anne K.
Torta, Federico T.
Wenk, Markus R.
Dawson, Thomas L.
author_sort Ambaw, Yohannes Abere
collection PubMed
description Malassezia are common components of human skin, and as the dominant human skin eukaryotic microbe, they take part in complex microbe–host interactions. Other phylogenetically related fungi (including within Ustilagomycotina) communicate with their plant host through bioactive oxygenated polyunsaturated fatty acids, generally known as oxylipins, by regulating the plant immune system to increase their virulence. Oxylipins are similar in structure and function to human eicosanoids, which modulate the human immune system. This study reports the development of a highly sensitive mass-spectrometry-based method to capture and quantify bioactive oxygenated polyunsaturated fatty acids from the human skin surface and in vitro Malassezia cultures. It confirms that Malassezia are capable of synthesizing eicosanoid-like lipid mediators in vitro in a species dependent manner, many of which are found on human skin. This method enables sensitive identification and quantification of bioactive lipid mediators from human skin that may be derived from metabolic pathways shared between skin and its microbial residents. This enables better cross-disciplinary and detailed studies to dissect the interaction between Malassezia and human skin, and to identify potential intervention points to promote or abrogate inflammation and to improve human skin health.
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spelling pubmed-85387392021-10-24 Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia Ambaw, Yohannes Abere Pagac, Martin P. Irudayaswamy, Antony S. Raida, Manfred Bendt, Anne K. Torta, Federico T. Wenk, Markus R. Dawson, Thomas L. Metabolites Article Malassezia are common components of human skin, and as the dominant human skin eukaryotic microbe, they take part in complex microbe–host interactions. Other phylogenetically related fungi (including within Ustilagomycotina) communicate with their plant host through bioactive oxygenated polyunsaturated fatty acids, generally known as oxylipins, by regulating the plant immune system to increase their virulence. Oxylipins are similar in structure and function to human eicosanoids, which modulate the human immune system. This study reports the development of a highly sensitive mass-spectrometry-based method to capture and quantify bioactive oxygenated polyunsaturated fatty acids from the human skin surface and in vitro Malassezia cultures. It confirms that Malassezia are capable of synthesizing eicosanoid-like lipid mediators in vitro in a species dependent manner, many of which are found on human skin. This method enables sensitive identification and quantification of bioactive lipid mediators from human skin that may be derived from metabolic pathways shared between skin and its microbial residents. This enables better cross-disciplinary and detailed studies to dissect the interaction between Malassezia and human skin, and to identify potential intervention points to promote or abrogate inflammation and to improve human skin health. MDPI 2021-10-13 /pmc/articles/PMC8538739/ /pubmed/34677414 http://dx.doi.org/10.3390/metabo11100700 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ambaw, Yohannes Abere
Pagac, Martin P.
Irudayaswamy, Antony S.
Raida, Manfred
Bendt, Anne K.
Torta, Federico T.
Wenk, Markus R.
Dawson, Thomas L.
Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia
title Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia
title_full Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia
title_fullStr Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia
title_full_unstemmed Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia
title_short Host/Malassezia Interaction: A Quantitative, Non-Invasive Method Profiling Oxylipin Production Associates Human Skin Eicosanoids with Malassezia
title_sort host/malassezia interaction: a quantitative, non-invasive method profiling oxylipin production associates human skin eicosanoids with malassezia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538739/
https://www.ncbi.nlm.nih.gov/pubmed/34677414
http://dx.doi.org/10.3390/metabo11100700
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