Cargando…
An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode
Due to the severe acute respiratory syndrome coronavirus (SARS-CoV-2, also called coronavirus disease 2019 (COVID-19)) pandemic starting in early 2020, all social activities ceased in order to combat its high transmission rate. Since vaccination combats one aspect for halting the spread of the virus...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538778/ https://www.ncbi.nlm.nih.gov/pubmed/34683225 http://dx.doi.org/10.3390/mi12101171 |
_version_ | 1784588588461064192 |
---|---|
author | Pang, Sow-Neng Lin, Yu-Lun Yu, Kai-Jie Chiou, Yueh-Er Leung, Wai-Hung Weng, Wen-Hui |
author_facet | Pang, Sow-Neng Lin, Yu-Lun Yu, Kai-Jie Chiou, Yueh-Er Leung, Wai-Hung Weng, Wen-Hui |
author_sort | Pang, Sow-Neng |
collection | PubMed |
description | Due to the severe acute respiratory syndrome coronavirus (SARS-CoV-2, also called coronavirus disease 2019 (COVID-19)) pandemic starting in early 2020, all social activities ceased in order to combat its high transmission rate. Since vaccination combats one aspect for halting the spread of the virus, the biosensor community has looked at another aspect of reducing the burden of the COVID-19 pandemic on society by developing biosensors that incorporate point-of-care (POC) testing and the rapid identification of those affected in order to deploy appropriate measures. In this study, we aim first to propose a screen-printed carbon electrode (SPCE)-based electrochemical biosensor that meets the ASSURED criteria (i.e., affordable, sensitive, specific, user-friendly, rapid, equipment-free, and deliverable) for POC testing, but more importantly, we describe the novelty of our biosensor’s modifiability that uses custom dual probes made from target nucleic acid sequences. Additionally, regarding the sensitivity of the biosensor, the lowest sample concentration was 10 pM (p = 0.0257) without amplification, which might challenge the traditional technique of reverse transcriptase-polymerase chain reaction (RT-PCR). The purpose of this study is to develop a means of diagnostics for the current pandemic as well as to provide an established POC platform for future epidemics. |
format | Online Article Text |
id | pubmed-8538778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-85387782021-10-24 An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode Pang, Sow-Neng Lin, Yu-Lun Yu, Kai-Jie Chiou, Yueh-Er Leung, Wai-Hung Weng, Wen-Hui Micromachines (Basel) Communication Due to the severe acute respiratory syndrome coronavirus (SARS-CoV-2, also called coronavirus disease 2019 (COVID-19)) pandemic starting in early 2020, all social activities ceased in order to combat its high transmission rate. Since vaccination combats one aspect for halting the spread of the virus, the biosensor community has looked at another aspect of reducing the burden of the COVID-19 pandemic on society by developing biosensors that incorporate point-of-care (POC) testing and the rapid identification of those affected in order to deploy appropriate measures. In this study, we aim first to propose a screen-printed carbon electrode (SPCE)-based electrochemical biosensor that meets the ASSURED criteria (i.e., affordable, sensitive, specific, user-friendly, rapid, equipment-free, and deliverable) for POC testing, but more importantly, we describe the novelty of our biosensor’s modifiability that uses custom dual probes made from target nucleic acid sequences. Additionally, regarding the sensitivity of the biosensor, the lowest sample concentration was 10 pM (p = 0.0257) without amplification, which might challenge the traditional technique of reverse transcriptase-polymerase chain reaction (RT-PCR). The purpose of this study is to develop a means of diagnostics for the current pandemic as well as to provide an established POC platform for future epidemics. MDPI 2021-09-29 /pmc/articles/PMC8538778/ /pubmed/34683225 http://dx.doi.org/10.3390/mi12101171 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Pang, Sow-Neng Lin, Yu-Lun Yu, Kai-Jie Chiou, Yueh-Er Leung, Wai-Hung Weng, Wen-Hui An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode |
title | An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode |
title_full | An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode |
title_fullStr | An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode |
title_full_unstemmed | An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode |
title_short | An Effective SARS-CoV-2 Electrochemical Biosensor with Modifiable Dual Probes Using a Modified Screen-Printed Carbon Electrode |
title_sort | effective sars-cov-2 electrochemical biosensor with modifiable dual probes using a modified screen-printed carbon electrode |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538778/ https://www.ncbi.nlm.nih.gov/pubmed/34683225 http://dx.doi.org/10.3390/mi12101171 |
work_keys_str_mv | AT pangsowneng aneffectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT linyulun aneffectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT yukaijie aneffectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT chiouyueher aneffectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT leungwaihung aneffectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT wengwenhui aneffectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT pangsowneng effectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT linyulun effectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT yukaijie effectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT chiouyueher effectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT leungwaihung effectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode AT wengwenhui effectivesarscov2electrochemicalbiosensorwithmodifiabledualprobesusingamodifiedscreenprintedcarbonelectrode |