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PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children

Invasive Streptococcus pneumoniae disease is preceded by asymptomatic nasopharyngeal carriage. Measuring carriage in healthy populations provides data on what serotypes are present in communities, which is of interest in the era of polyvalent pneumococcal conjugate vaccines. Nasopharyngeal swabs fro...

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Autores principales: Ricketson, Leah J., Lidder, Ravinder, Thorington, Robyn, Martin, Irene, Vanderkooi, Otto G., Sadarangani, Manish, Kellner, James D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538797/
https://www.ncbi.nlm.nih.gov/pubmed/34683437
http://dx.doi.org/10.3390/microorganisms9102116
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author Ricketson, Leah J.
Lidder, Ravinder
Thorington, Robyn
Martin, Irene
Vanderkooi, Otto G.
Sadarangani, Manish
Kellner, James D.
author_facet Ricketson, Leah J.
Lidder, Ravinder
Thorington, Robyn
Martin, Irene
Vanderkooi, Otto G.
Sadarangani, Manish
Kellner, James D.
author_sort Ricketson, Leah J.
collection PubMed
description Invasive Streptococcus pneumoniae disease is preceded by asymptomatic nasopharyngeal carriage. Measuring carriage in healthy populations provides data on what serotypes are present in communities, which is of interest in the era of polyvalent pneumococcal conjugate vaccines. Nasopharyngeal swabs from a survey of 682 and 800 healthy children in 2016 and 2018, respectively, were analyzed by culture and Quellung reaction to determine rates of carriage and serotypes. All swabs from 2016 and 300 randomly selected swabs from 2018 were then analyzed using real-time semi-quantitative PCR (qPCR) to detect S. pneumoniae gene targets lytA, piaA, and SP2020 and determine serotype. There were 71 (10.4%) and 68 (8.5%) culture positive samples in 2016 and 2018, respectively. All of these were also positive by qPCR except one that was equivocal. In total, 46.0% of 2016 swabs were positive by qPCR. In 2018, results from the selected sample extrapolated to the complete sample showed 49.0% positive by qPCR. PCV13 serotypes were detected in 29.3% and 21.7% of S. pneumoniae qPCR positive samples from 2016 and 2018, respectively; compared with only 8.4% and 6.0% PCV13 serotypes detected by Quellung reaction in culture positive samples. Compared with culture, qPCR detected S. pneumoniae more frequently. Further, qPCR serotyping detected PCV13 serotypes in a larger proportion of samples than culture and Quellung reaction did, showing that, despite established universal childhood PCV13 immunization, vaccine serotypes can still be detected in a large proportion of young children.
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spelling pubmed-85387972021-10-24 PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children Ricketson, Leah J. Lidder, Ravinder Thorington, Robyn Martin, Irene Vanderkooi, Otto G. Sadarangani, Manish Kellner, James D. Microorganisms Article Invasive Streptococcus pneumoniae disease is preceded by asymptomatic nasopharyngeal carriage. Measuring carriage in healthy populations provides data on what serotypes are present in communities, which is of interest in the era of polyvalent pneumococcal conjugate vaccines. Nasopharyngeal swabs from a survey of 682 and 800 healthy children in 2016 and 2018, respectively, were analyzed by culture and Quellung reaction to determine rates of carriage and serotypes. All swabs from 2016 and 300 randomly selected swabs from 2018 were then analyzed using real-time semi-quantitative PCR (qPCR) to detect S. pneumoniae gene targets lytA, piaA, and SP2020 and determine serotype. There were 71 (10.4%) and 68 (8.5%) culture positive samples in 2016 and 2018, respectively. All of these were also positive by qPCR except one that was equivocal. In total, 46.0% of 2016 swabs were positive by qPCR. In 2018, results from the selected sample extrapolated to the complete sample showed 49.0% positive by qPCR. PCV13 serotypes were detected in 29.3% and 21.7% of S. pneumoniae qPCR positive samples from 2016 and 2018, respectively; compared with only 8.4% and 6.0% PCV13 serotypes detected by Quellung reaction in culture positive samples. Compared with culture, qPCR detected S. pneumoniae more frequently. Further, qPCR serotyping detected PCV13 serotypes in a larger proportion of samples than culture and Quellung reaction did, showing that, despite established universal childhood PCV13 immunization, vaccine serotypes can still be detected in a large proportion of young children. MDPI 2021-10-08 /pmc/articles/PMC8538797/ /pubmed/34683437 http://dx.doi.org/10.3390/microorganisms9102116 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ricketson, Leah J.
Lidder, Ravinder
Thorington, Robyn
Martin, Irene
Vanderkooi, Otto G.
Sadarangani, Manish
Kellner, James D.
PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children
title PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children
title_full PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children
title_fullStr PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children
title_full_unstemmed PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children
title_short PCR and Culture Analysis of Streptococcus pneumoniae Nasopharyngeal Carriage in Healthy Children
title_sort pcr and culture analysis of streptococcus pneumoniae nasopharyngeal carriage in healthy children
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538797/
https://www.ncbi.nlm.nih.gov/pubmed/34683437
http://dx.doi.org/10.3390/microorganisms9102116
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