Cargando…

Tracking the Transcription Kinetic of SARS-CoV-2 in Human Cells by Reverse Transcription-Droplet Digital PCR

Viral transcription is an essential step of SARS-CoV-2 infection after invasion into the target cells. Antiviral drugs such as remdesivir, which is used to treat COVID-19 patients, targets the viral RNA synthesis. Understanding the mechanism of viral transcription may help to develop new therapeutic...

Descripción completa

Detalles Bibliográficos
Autores principales:	Au, Ka-Ki, Chen, Chunke, Chan, Yee-Man, Wong, Winsome Wing Sum, Lv, Huibin, Mok, Chris Ka Pun, Chow, Chun-Kin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2021
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8538813/ https://www.ncbi.nlm.nih.gov/pubmed/34684223 http://dx.doi.org/10.3390/pathogens10101274

Ejemplares similares

Long Distance Reverse-Transcription PCR
por: Thiel, Volker, et al.
Publicado: (2002)

Application of One-Step Reverse Transcription Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical Specimens
por: Mairiang, Dumrong, et al.
Publicado: (2021)

Development of a reverse transcription droplet digital PCR (RT-ddPCR) assay for sensitive detection of simian immunodeficiency virus (SIV)
por: Long, Samuel, et al.
Publicado: (2021)

Comparison of reverse-transcription qPCR and droplet digital PCR for the detection of SARS-CoV-2 in clinical specimens of hospitalized patients
por: Li, Jingyuan, et al.
Publicado: (2022)

Quantitation and integrity evaluation of RNA genome in lentiviral vectors by direct reverse transcription-droplet digital PCR (direct RT-ddPCR)
por: He, Zhiyong, et al.
Publicado: (2023)

Comparison of Reverse Transcription (RT)-Quantitative PCR and RT-Droplet Digital PCR for Detection of Genomic and Subgenomic SARS-CoV-2 RNA
por: Morón-López, Sara, et al.
Publicado: (2023)

Printing 2-Dimentional Droplet Array for Single-Cell Reverse Transcription Quantitative PCR Assay with a Microfluidic Robot
por: Zhu, Ying, et al.
Publicado: (2015)

Detection and Quantification of Apple Stem Grooving Virus in Micropropagated Apple Plantlets Using Reverse-Transcription Droplet Digital PCR
por: Kim, Sung-Woong, et al.
Publicado: (2022)

Development of a reverse-transcription droplet digital PCR method for quantitative detection of Cucumber green mottle mosaic virus
por: Tian, Yimin, et al.
Publicado: (2022)

Selective control of primer usage in multiplex one-step reverse transcription PCR
por: Hidalgo Ashrafi, Elena, et al.
Publicado: (2009)

Adaptation of Droplet Digital PCR-Based HIV Transcription Profiling to Digital PCR and Association of HIV Transcription and Total or Intact HIV DNA
por: Tumpach, Carolin, et al.
Publicado: (2023)

Droplet digital PCR quantifies host inflammatory transcripts in feces reliably and reproducibly
por: Stauber, Jennifer, et al.
Publicado: (2016)

Evaluation of relative quantification of alternatively spliced transcripts using droplet digital PCR
por: Van Heetvelde, Mattias, et al.
Publicado: (2017)

Coordinated Implementation of Chikungunya Virus Reverse Transcription–PCR
por: Panning, Marcus, et al.
Publicado: (2009)

Noninvasive prenatal paternity testing by means of SNP‐based targeted sequencing
por: Tam, Jacqueline Chor Wing, et al.
Publicado: (2020)

A reverse-transcription droplet digital PCR assay to detect and quantify SARS-CoV-2 RNA in upper respiratory tract specimens
por: Milosevic, Dragana, et al.
Publicado: (2022)

Standardized positive controls for detection of norovirus by reverse transcription PCR
por: Lee, Sung-Geun, et al.
Publicado: (2011)

Effective Amplification of 20-kb DNA by Reverse Transcription PCR()
por: Thiel, Volker, et al.
Publicado: (1997)

Reverse transcription PCR to detect low density malaria infections
por: Christensen, Peter, et al.
Publicado: (2022)

Validation of a One-Step Reverse Transcription-Droplet Digital PCR (RT-ddPCR) Approach to Detect and Quantify SARS-CoV-2 RNA in Nasopharyngeal Swabs
por: Mio, Catia, et al.
Publicado: (2021)

Clinical evaluation of the effectiveness of fusion‐induced asymmetric transcription assay‐based reverse transcription droplet digital PCR for ALK detection in formalin‐fixed paraffin‐embedded samples from lung cancer
por: Liu, Yuanyuan, et al.
Publicado: (2020)

Investigation of SARS-CoV-2 on Ocular Surface of Coronavirus Disease 2019 Patients Using One-Step Reverse-Transcription Droplet Digital PCR
por: Zhang, Xian, et al.
Publicado: (2021)

Development of one-step TaqMan(®) real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses
por: Lu, Zhengchun, et al.
Publicado: (2012)

Reverse-transcription quantitative PCR directly from cells without RNA extraction and without isothermal reverse-transcription: a ‘zero-step’ RT-qPCR protocol
por: Chovancova, Petra, et al.
Publicado: (2017)

Response to clinical evaluation of the effectiveness of fusion‐induced asymmetric transcription assay‐based reverse transcription droplet digital PCR for ALK detection in formalin‐fixed paraffin‐embedded samples from lung cancer
por: Peñaloza Coronas, Cynthia, et al.
Publicado: (2021)

Reference genes for reverse transcription quantitative PCR in canine brain tissue
por: Stassen, Quirine E. M., et al.
Publicado: (2015)

Reverse transcription-quantitative PCR (RT-qPCR) without the need for prior removal of DNA
por: Đermić, Damir, et al.
Publicado: (2023)

Interaction of Influenza A Nucleoprotein with Host hnRNP-C Is Implicated in Viral Replication
por: Tang, Yun-Sang, et al.
Publicado: (2022)

Accurate quantification of SARS-CoV-2 RNA by isotope dilution mass spectrometry and providing a correction of reverse transcription efficiency in droplet digital PCR
por: Niu, Chunyan, et al.
Publicado: (2022)

Tracking transcripts
por: LeBrasseur, Nicole
Publicado: (2004)

Mink infection with influenza A viruses: an ignored intermediate host?
por: Mok, Chris Ka Pun, et al.
Publicado: (2023)

Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses
por: Patel, Pranav, et al.
Publicado: (2013)

Pan-Piscine Orthoreovirus (PRV) Detection Using Reverse Transcription Quantitative PCR
por: Zhao, Julie, et al.
Publicado: (2021)

Detecting the Neuraminidase R294K Mutation in Avian Influenza A (H7N9) Virus Using Reverse Transcription Droplet Digital PCR Method
por: Lou, Xiuyu, et al.
Publicado: (2023)

Development, Validation, and Application of Reverse Transcription Real-Time and Droplet Digital PCR Assays for the Detection of the Potyviruses Watermelon Mosaic Virus and Zucchini Yellow Mosaic Virus in Cucurbits
por: Luigi, Marta, et al.
Publicado: (2023)

A Randomized Clinical Trial Using CoronaVac or BNT162b2 Vaccine as a Third Dose in Adults Vaccinated with Two Doses of CoronaVac
por: Mok, Chris Ka Pun, et al.
Publicado: (2022)

Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR
por: Sales, Mariana Lázaro, et al.
Publicado: (2023)

Comparison of Target Enrichment Platforms for Circulating Tumor DNA Detection
por: Lam, So Ngo, et al.
Publicado: (2020)

Clinical Evaluation of Self-Collected Saliva by Quantitative Reverse Transcription-PCR (RT-qPCR), Direct RT-qPCR, Reverse Transcription–Loop-Mediated Isothermal Amplification, and a Rapid Antigen Test To Diagnose COVID-19
por: Nagura-Ikeda, Mayu, et al.
Publicado: (2020)

Do demographic factors and a health-promoting lifestyle influence the self-rated health of college nursing students?
por: CHOW, Susan Ka Yee, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_128513cumqs9v6lkacpt7ebdr2